LOCUS       sequence-329498-        4464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeriDDX5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Harel I, Chen YR, Ziv I, Singh PP, Heinzer D, Navarro Negredo P, 
            Goshtchevsky U, Wang W, Astre G, Moses E, McKay A, Machado BE, 
            Hebestreit K, Yin S, Sanchez Alvarado A, Jarosz DF, Brunet A
  TITLE     Identification of protein aggregates in the aging vertebrate brain 
            with prion-like and phase-separation properties.
  JOURNAL   Cell Rep. 2024 Jun 25;43(6):112787. doi: 
            10.1016/j.celrep.2023.112787. Epub 2024 May 28.
  PUBMED    38810650
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-06-25"; volume: "43(6):112787. doi"; pages: " 
            10.1016/j.celrep.2023.112787. Epub 2024 May 28"
COMMENT     Sequence Label: pDONR221-DDX5
FEATURES             Location/Qualifiers
     source          1..4464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2589..2688)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2705..2724)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2706..2724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2729..2745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2729..2745)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2847..2866)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2858..3667
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2925..2944)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3814..4402
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4303..4322
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgcc tggatttgca gacagagacc gcggcagaga
      721 tagagggtat gggggtggcc cccctcggtt tggtggcggt gggggaggag gcggcaacag
      781 aggaggacct ctgctgggaa agtttggaaa ccctggtgag aggctgcgca agaagcactg
      841 gaaccttgat gaactaccaa aatttcaaaa gaatttctac caggaacacc cagatgtcac
      901 acacagacca ctgcaggagg ttgaccagta ccgcagaaac aaagaagtta cagttaaagg
      961 cagagattgt cccaaaccga ttgtaaaatt ccatgaagct gcttttccca actacgtcat
     1021 ggatgtcatt gtcaaacaga actggactga accaactccg atccagtctc aggggtggcc
     1081 cattgtcctg agtggaaaag acatggtcgg catcgctcaa acggggtctg ggaagacttt
     1141 ggcatatctt ctgcctgcag tcgttcacat ccaacatcag cccttcttgg agcacggaga
     1201 cgggcctatt tgcttggtgc tggcaccaac ccgtgagctg gctcagcagg tgcaacaggt
     1261 ggctgctgag tacggcaggg cctcccgtct caagagtaca tgcatctatg gtggtgctcc
     1321 caaaggacca caaatccggg accttgagag gggtgttgag atttgcatcg ccaccccagg
     1381 tcgcctcatt gatttcctgg aatgtggtaa aactaatttg cgtcgttgca cctatctagt
     1441 gctggatgaa gctgaccgca tgctggacat gggctttgaa cctcagatcc gcaagatagt
     1501 ggatcaaata cggccagacc gtcagactct gatgtggagt gccacctggc ctaaagaagt
     1561 tcgtcagctg gctgaggact tcttgaagga ttacgtccag atcaacattg gcgccctgca
     1621 gctcagcgcc aatcataaca tcctgcaaat agtagatgtt tgcaatgact tagaaaagga
     1681 agacaaactg attcggttgc tggaggagat catgagtgaa aaggaaaaca agaccatcat
     1741 ttttgtggag accaaaaggc gttgtgatga gctcaccagg aggatgagaa gagacggatg
     1801 gcctgcaatg ggaatccatg gagataagag ccagcaggag agggactggg tccttaacga
     1861 gttcagattt ggcaaagctc cgattctcat tgctacagac gtggcctccc ggggcttaga
     1921 tgtggaggat gtgaaatttg tcatcaatta tgactaccct aactcctctg aggattatat
     1981 ccaccgcatt ggacgcacag cccgaagtca aaaaacgggc acggcctaca ccttcttcac
     2041 ccccaacaac atgaaacagg caggtgacct gatctctgtg ctccgtgaag ccaaccaggc
     2101 catcaacccc aaactgatcc agatggctga ggacagagga ggtcgcggta gggggggaag
     2161 aggtggctac aaggatgacc gtcgcgatag gtattctggg ggtgggagga gtaattttgg
     2221 cggtagtggc tttagagaca gggaccatga cagaggtttc ggcaatgggc cgaagaccac
     2281 atttggtggc agcaaggccc aaaatagtgg caattatggt ggcaacagtg gtaactcttg
     2341 tggtagctat ggcaacaata actacaacaa cagtaatgga cagggtagtt ttagtggtcc
     2401 acctaaccaa ggtggctttg ggaaccaggg cttccaagcc cctcctcaat ttggtggcat
     2461 gcagagggct gcacagaatg gtgttaatca tccaccattt ccttttaacg ctcagccacc
     2521 cccgccacag aaccagcagc cccctccacc aatggtgccc tacccaattc caccaccgtt
     2581 tccacaagac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     2641 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     2701 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     2761 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa
     2821 actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac
     2881 gtcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg
     2941 cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc
     3001 agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt
     3061 cagactaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac
     3121 tcctgatgat gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt
     3181 agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg
     3241 gttgcattcg attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc
     3301 tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg
     3361 taatggctgg cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc
     3421 ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa
     3481 attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc
     3541 catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa
     3601 atatggtatt gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt
     3661 tttctaatca gaattggtta attggttgta acactggcag agcattacgc tgacttgacg
     3721 ggacggcgca agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     3781 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3841 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3901 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3961 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4021 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4081 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4141 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4201 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4261 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4321 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4381 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     4441 ttgctggcct tttgctcaca tgtt
//
