LOCUS       sequence-329499-        4167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri DDX5-Delta-PrD(1-535) mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Harel I, Chen YR, Ziv I, Singh PP, Heinzer D, Navarro Negredo P, 
            Goshtchevsky U, Wang W, Astre G, Moses E, McKay A, Machado BE, 
            Hebestreit K, Yin S, Sanchez Alvarado A, Jarosz DF, Brunet A
  TITLE     Identification of protein aggregates in the aging vertebrate brain 
            with prion-like and phase-separation properties.
  JOURNAL   Cell Rep. 2024 Jun 25;43(6):112787. doi: 
            10.1016/j.celrep.2023.112787. Epub 2024 May 28.
  PUBMED    38810650
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-06-25"; volume: "43(6):112787. doi"; pages: " 
            10.1016/j.celrep.2023.112787. Epub 2024 May 28"
COMMENT     Sequence Label: pDONR221-DDX5-Delta-PrD
FEATURES             Location/Qualifiers
     source          1..4167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2292..2391)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2408..2427)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2409..2427)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2432..2448)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2432..2448)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2550..2569)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2561..3370
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2628..2647)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3517..4105
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4006..4025
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgcc tggatttgca gacagagacc gcggcagaga
      721 tagagggtat gggggtggcc cccctcggtt tggtggcggt gggggaggag gcggcaacag
      781 aggaggacct ctgctgggaa agtttggaaa ccctggtgag aggctgcgca agaagcactg
      841 gaaccttgat gaactaccaa aatttcaaaa gaatttctac caggaacacc cagatgtcac
      901 acacagacca ctgcaggagg ttgaccagta ccgcagaaac aaagaagtta cagttaaagg
      961 cagagattgt cccaaaccga ttgtaaaatt ccatgaagct gcttttccca actacgtcat
     1021 ggatgtcatt gtcaaacaga actggactga accaactccg atccagtctc aggggtggcc
     1081 cattgtcctg agtggaaaag acatggtcgg catcgctcaa acggggtctg ggaagacttt
     1141 ggcatatctt ctgcctgcag tcgttcacat ccaacatcag cccttcttgg agcacggaga
     1201 cgggcctatt tgcttggtgc tggcaccaac ccgtgagctg gctcagcagg tgcaacaggt
     1261 ggctgctgag tacggcaggg cctcccgtct caagagtaca tgcatctatg gtggtgctcc
     1321 caaaggacca caaatccggg accttgagag gggtgttgag atttgcatcg ccaccccagg
     1381 tcgcctcatt gatttcctgg aatgtggtaa aactaatttg cgtcgttgca cctatctagt
     1441 gctggatgaa gctgaccgca tgctggacat gggctttgaa cctcagatcc gcaagatagt
     1501 ggatcaaata cggccagacc gtcagactct gatgtggagt gccacctggc ctaaagaagt
     1561 tcgtcagctg gctgaggact tcttgaagga ttacgtccag atcaacattg gcgccctgca
     1621 gctcagcgcc aatcataaca tcctgcaaat agtagatgtt tgcaatgact tagaaaagga
     1681 agacaaactg attcggttgc tggaggagat catgagtgaa aaggaaaaca agaccatcat
     1741 ttttgtggag accaaaaggc gttgtgatga gctcaccagg aggatgagaa gagacggatg
     1801 gcctgcaatg ggaatccatg gagataagag ccagcaggag agggactggg tccttaacga
     1861 gttcagattt ggcaaagctc cgattctcat tgctacagac gtggcctccc ggggcttaga
     1921 tgtggaggat gtgaaatttg tcatcaatta tgactaccct aactcctctg aggattatat
     1981 ccaccgcatt ggacgcacag cccgaagtca aaaaacgggc acggcctaca ccttcttcac
     2041 ccccaacaac atgaaacagg caggtgacct gatctctgtg ctccgtgaag ccaaccaggc
     2101 catcaacccc aaactgatcc agatggctga ggacagagga ggtcgcggta gggggggaag
     2161 aggtggctac aaggatgacc gtcgcgatag gtattctggg ggtgggagga gtaattttgg
     2221 cggtagtggc tttagagaca gggaccatga cagaggtttc ggcaatgggc cgaagaccac
     2281 atttggtggc gacccagctt tcttgtacaa agttggcatt ataagaaagc attgcttatc
     2341 aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt gccatccagc
     2401 tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc agctctggcc
     2461 cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat
     2521 aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga
     2581 aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc
     2641 tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc
     2701 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat
     2761 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg
     2821 tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt
     2881 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg
     2941 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct
     3001 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga
     3061 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc
     3121 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg
     3181 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct
     3241 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca
     3301 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga
     3361 gtttttctaa tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg
     3421 acgggacggc gcaagctcat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg
     3481 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3541 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3601 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3661 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3721 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3781 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3841 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3901 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3961 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4021 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4081 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4141 cttttgctgg ccttttgctc acatgtt
//
