LOCUS       sequence-329507-        4218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri EIF3D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-EIF3D
FEATURES             Location/Qualifiers
     source          1..4218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2343..2442)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2459..2478)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2460..2478)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2483..2499)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2483..2499)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2601..2620)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2612..3421
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2679..2698)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3568..4156
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4057..4076
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatggc aaagtttcac gcccccgtga tccaggacaa
      721 cccgtctgga tggggtccgt gtgcagtccc ggagaagttc aaagatatgc cctaccagcc
      781 tttcagcaaa ggagacagac tgggaaaggt tgctgactgg actggagcta cttaccaaga
      841 caagagatac acgaataaat attcctctca gtttggaggt gggagtcagt atgcctactt
      901 ccatgaggaa gatgagacca gcttccagct tgtggacact gctaagaccc agaagacggc
      961 gtaccagagg aaccgcatgc ggtttgctca gaggaacctt cgcagggaca aggaccgtag
     1021 gaacttgact cagttcaaca tgcagacact tcctaagagt gccaagcaga aggagagaga
     1081 tcgtttgcgt ctgcagaaga agttccagaa gcagtttggt gtccgtcaga agtgggatca
     1141 aaaatctcag gcccagctga agcccagaga ctcctctgtg gaggtgagga gtgactggga
     1201 ggtgaaggag gagatggact tccccagact gatgaagatg cgatacatgg aggtttctga
     1261 ccctgttgac atcgagtgct gtggtgcgtt ggagtactac gataaagcgt tcgatcggat
     1321 caccacccgc aacgagaagc agctgaagag catcaagagg attttccaca ccgtcaccac
     1381 caccgatgat ccggtcatcc ggaagctggc gaagactcag ggcaacgtgt ttgctacaga
     1441 cgctatcttg gccacattga tgtgctgcac acgctccgtc aactcctggg acatcatcgt
     1501 gcagagagtc ggcaacaagc tcttctttga caagagggac aactctgact ttgatttgct
     1561 aactgtgagt gaaaccgcca acgagcctcc tcaagatgag ggaaactcct ttaactctcc
     1621 tcgtaacctg gcgatggagg ccacctacat caaccacaac ttcagccagc agtgtttacg
     1681 tgtgggagga gaacggtaca agttcccaaa ccctaaccca tttgtggagg aagacatgga
     1741 caagagcgag gtggcgtctg tggcctacag gtaccgtcac tggaagctcg gggatgacat
     1801 cgacctgatc atccgctgtg aacatgacgg agtgatgact ggagcgaatg gagaggtgtc
     1861 cttcatcaac gtcaagaccc tgaacgagtg ggactccagg tattgtaatg gagtggattg
     1921 gcgccagaag ttggactctc agcggggagc cgttctggcc accgagctga agaacaacag
     1981 ctacaaactg gcccgctgga cctgttgtgc catgctggcc gggtcggagt atctgaaact
     2041 ggggtacgtg tctcggtacc acgtgaagga ttcttctcgt cacgtcatcc tgggaaccca
     2101 gcagttcaaa cccaacgagt ttgccagcca gatcaacctc agcatggaga acgcctgggg
     2161 gatcctccgc tgcgtcatcg acatctgccg caagctggat gagggcaagt acctgatcct
     2221 gaaggacccc aacaagcaag tgattcgagt gtacagcctg cctgaaggta ccttcagctc
     2281 tgatgaggag gaggaggaag aggaggatga agaggatgag gaggaggaag atgaagagaa
     2341 cgacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     2401 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     2461 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2521 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     2581 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     2641 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     2701 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     2761 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     2821 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     2881 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     2941 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     3001 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     3061 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     3121 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     3181 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     3241 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     3301 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     3361 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     3421 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     3481 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     3541 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3601 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3661 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3721 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3781 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3841 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3901 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3961 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4021 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4081 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4141 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4201 gccttttgct cacatgtt
//
