LOCUS       sequence-329511-       10543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mCerulean3 fused to Bcl-2 family protein, Bcl-W, in a 
            plasmid compatable for lentiviral infection. Used to make stable 
            BMK-DKO cell lines..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10543)
  AUTHORS   Osterlund EJ, Hirmiz N, Pemberton JM, Nougarede A, Liu Q, Leber B, 
            Fang Q, Andrews DW
  TITLE     Efficacy and specificity of inhibitors of BCL-2 family protein 
            interactions assessed by affinity measurements in live cells.
  JOURNAL   Sci Adv. 2022 Apr 22;8(16):eabm7375. doi: 10.1126/sciadv.abm7375. 
            Epub 2022 Apr 20.
  PUBMED    35442739
REFERENCE   2  (bases 1 to 10543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2022-04-22"; volume: "8(16):eabm7375. doi"; pages: " 
            10.1126/sciadv.abm7375. Epub 2022 Apr 20"
COMMENT     Sequence Label: mCerulean3-Bcl-W-FUGW
FEATURES             Location/Qualifiers
     source          1..10543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(103..122)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        294..673
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        675..873
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     828..848
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             891..1071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1118..1243
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1736..1969
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2154..2198
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2496..2613
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2678..3889
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3726..3745
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(3918..3920,3921..3923,3924..4634)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3963..3984)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4224..4243)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4571..4592
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5291..5879
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5344..5364)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5762..5773)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5882..5898)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5883..5899)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6404..6584
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6610..6627)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6616..6840
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6886..7314
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6973..6992)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7183..7204
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7323..7343)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7328..7657
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7508..7643
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7570..7589
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7705..7752
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7771..8145
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8275..8396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8312..8331)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8366..8385
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8445..8461)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8445..8461)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8458..8480)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8469..8485
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8493..8499,8500..8517,8518..8523))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8538..8559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8676..8693)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8847..9435)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8927..8946)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9606..10397,10398..10466))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10229..10248
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10467..10543,1..28))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
       61 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
      121 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      181 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
      241 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      301 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      361 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      421 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      481 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      541 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      601 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      661 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      721 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      781 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      841 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
      901 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
      961 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     1021 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     1081 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     1141 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     1201 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     1261 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     1321 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     1381 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     1441 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     1501 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     1561 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     1621 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1681 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1741 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     1801 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1861 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1921 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1981 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     2041 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     2101 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     2161 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     2221 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     2281 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     2341 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     2401 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     2461 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     2521 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2581 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     2641 ccagtttggt taattaaccc gtgtcggctc cagatctggc ctccgcgccg ggttttggcg
     2701 cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga agggcgcagc
     2761 gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc ataagactcg
     2821 gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg tgactctagg
     2881 gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct tctcggcgat
     2941 tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga cgcgccgggt
     3001 gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt tgtggatcgc
     3061 tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc gtggccgccg
     3121 ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt ctgggtccgc
     3181 gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg gctgttcccg
     3241 agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca aggtgggggg
     3301 catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga aagctcttat
     3361 tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg tcactgactg
     3421 gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg ttgggcagtg
     3481 cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg ttctgttggc
     3541 ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc tccgtcgcag
     3601 gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg acctctggtg
     3661 aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct atcttcttaa
     3721 gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt tttgtgaagt
     3781 tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc agtgttagac
     3841 tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg aagcttgggc
     3901 tgcaggtcga ctctagaatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     3961 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     4021 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     4081 ccgtgccctg gcccaccctc gtgaccaccc tgagctgggg cgtgcagtgc ttcgcccgct
     4141 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     4201 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     4261 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     4321 gcaacatcct ggggcacaag ctggagtaca acgccatcca cggcaacgtc tatatcaccg
     4381 ccgacaagca gaagaacggc atcaaggcca acttcggcct caactgcaac atcgaggacg
     4441 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4501 tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga
     4561 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4621 acgagctgta caagtccgga ctcagatctc gagaaatggc gaccccagcc tcaaccccag
     4681 acacacgggc tctagtggct gactttgtag gctataagct gaggcagaag ggttatgtct
     4741 gtggagctgg ccctggggaa ggcccagccg ccgacccgct gcaccaagcc atgcgggctg
     4801 ctggagacga gtttgagacc cgtttccgcc gcaccttctc tgacctggcc gctcagctac
     4861 acgtgacccc aggctcagcc cagcaacgct tcacccaggt ttccgacgaa cttttccaag
     4921 ggggccctaa ctggggccgt cttgtggcat tctttgtctt tggggctgcc ctgtgtgctg
     4981 agagtgtcaa caaagaaatg gagcctttgg tgggacaagt gcaggattgg atggtggcct
     5041 acctggagac acgtctggct gactggatcc acagcagtgg gggctgggcg gagttcacag
     5101 ctctatacgg ggacggggcc ctggaggagg cacggcgtct gcgggagggg aactgggcat
     5161 cagtgaggac agtgctgacg ggggccgtgg cactgggggc cctggtaact gtaggggcct
     5221 tttttgctag caagtgagaa ttcttcgaag ctagcggatc cgttaacaat tcgatatcaa
     5281 gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5341 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5401 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5461 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5521 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5581 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5641 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     5701 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     5761 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5821 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca
     5881 tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     5941 agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc
     6001 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     6061 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     6121 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     6181 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     6241 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     6301 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     6361 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     6421 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6481 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6541 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
     6601 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     6661 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6721 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6781 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6841 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6901 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6961 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7021 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7081 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7141 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7201 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7261 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     7321 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     7381 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     7441 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     7501 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7561 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     7621 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     7681 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     7741 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     7801 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     7861 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     7921 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     7981 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     8041 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     8101 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     8161 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     8221 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     8281 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     8341 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8401 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     8461 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8521 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8581 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8641 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8701 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8761 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8821 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8881 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8941 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9001 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9061 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9121 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9181 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9241 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9301 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9361 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9421 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9481 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9541 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9601 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9661 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9721 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9781 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9841 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9901 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9961 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    10021 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10081 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10141 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10201 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10261 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10321 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10381 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10441 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    10501 cagggttatt gtctcatgag cggatacata tttgaatgta ttt
//
