LOCUS       sequence-329515-        3738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri FKBP8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-FKBP8(2of2)
FEATURES             Location/Qualifiers
     source          1..3738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1863..1962)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1979..1998)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1980..1998)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2003..2019)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2003..2019)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2121..2140)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2132..2941
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2199..2218)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3088..3676
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3577..3596
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgga gtcttcagct ggacctcctg ctgctaagaa
      721 gcatggacaa acctcgttgc tggacagcgg ggaggacttt gaggtgttgt gtgatgaaga
      781 catttatgac gaccttcctc ctctggaaga tgccgggggt ggaaacagta aaaaaacaga
      841 agatgcagaa aagaaaaggg ttgaagatcc agaaccttct ggcaacattg atgaatggct
      901 agatgtttta ggtaacgacc agctgaagaa aaaggtcctt gaagcaggga agggacgtga
      961 cagtcggcct cacaaaggac aggtcgtaaa gatccatctt aaaacacact tgatggatgg
     1021 tacacttgca caggagctat gtgatctcgc tttcactctg ggagatggtg atgtcatcca
     1081 ggcactggat ctgacagtgc agcttatgga aattggagag aaagcactca tccagacaga
     1141 tgcaaaatac gcatacggtg tccgaggaag tcttgatcct gacattccgc caaatgctga
     1201 gctttcctta gaggtggagc tgttggaggc tacagatgca cctgacttgg agctgcttcc
     1261 tccctcggag aaaatagccc tggctgttca gaagagggag agggggaatg ttcactacca
     1321 acgtggggat tatgcttttg cagtcaactc atatggcgtt gccctgcaga ttactgattc
     1381 ttgttccaaa gttgacatca gccccgagga ggagaacgag ctgttggatg tcaaagtaaa
     1441 gtgtctaaac aacatggctg cttctcagct gaagctggac cactatgacg cagcactgaa
     1501 gtcttgtgtt tcagcacttg aacatcaacc agagaatatt aaagcacttt ttcgaatggg
     1561 caaggttctg gctctgcagg ctgaatacac agaagccatt caaactttac ggaaggctct
     1621 gaagctggaa ccaagcaaca agacaattca cgctgagctc tccaagctgg tgaagaagca
     1681 ctcagagcag cggggagcag aacaggccat gtataagaag atgctgggca acccttctag
     1741 tagcagcgta cggaaacatc aggccaagtc ttcatggggt ctcagctgga agtggctttt
     1801 tggtgccaca gctgtagccc ttggtggcgt agctctatct gttgtcatag ctgccaggaa
     1861 tgacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     1921 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     1981 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2041 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     2101 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     2161 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     2221 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     2281 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     2341 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     2401 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     2461 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     2521 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     2581 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     2641 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     2701 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     2761 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     2821 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     2881 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     2941 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     3001 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     3061 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3121 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3181 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3241 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3301 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3361 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3421 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3481 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3541 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3601 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3661 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3721 gccttttgct cacatgtt
//
