LOCUS       sequence-329518-        4203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri G6PD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-G6PD(1of2)
FEATURES             Location/Qualifiers
     source          1..4203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2328..2427)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2444..2463)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2445..2463)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2468..2484)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2468..2484)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2586..2605)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2597..3406
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2664..2683)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3553..4141
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4042..4061
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatggg atctcgagcg agcgctgaac gacaccagcc
      721 tcaaacacac agccaggatc atcaagaacc gtcttcagtt aggagccccg caacagacga
      781 gaaaatgaac aacattccac tctctcgttc ggaggtgttt ggggagctga ggaaggagct
      841 ctacgaagat aaagagttcc atcagtctga tgtccacatg ttcatcatca tgggagcatc
      901 aggagatctg gcaaagaaaa agatctaccc aactctgtgg tggttgttca gagacggtct
      961 ccttcctgag cagacgtatt tcgtgggctt tgcccgctct gacctgacag tagatgccat
     1021 ccacaatgct tgcttgccat acctgaaggt ggcagataca gaggcagaac gtgtgtcggc
     1081 cttcttcagc aggaacacat acatcagtgg gaagtatgcc gatgacagct ccttcaccaa
     1141 actcaacaca cacatcctgt cgctgcctgg aggaagtggg gccaaccgcc tgttttacct
     1201 ggcgctgcca ccgacggtct accacgatgt taccaagaac atcagtcagc actgcatgag
     1261 cacaaaaggc tggaacaggg tgatcgtaga gaagccgttt ggacatgatc tccagagctc
     1321 agaggaactg tcctctcacc tctcgtctct cttcactgag gaccagatct accgtatcga
     1381 tcactacctg ggcaaagaga tggtgcagaa cctcatggtg ctaaggtttg gaaaccgcat
     1441 ctttgggccg atctggaaca gagacagcgt ggcctgtgtt gttcttacct ttaaggagcc
     1501 ttttggcact cagggacgag gaggctactt cgatgatttc ggcatcatcc gggatgtcat
     1561 gcagaaccac ttgctccaga tgctctgttt ggttgccatg gagaaaccag cctccaccag
     1621 ctctgacgac gtcagagatg aaaaggtgaa ggtgctgaag tgcatcactc cagtgtccat
     1681 gtcggatgtg gtcctgggtc agtatgtggg ggatccagag ggtgaaggag aagccaaact
     1741 tggttacctg gacgatccca cagtaccaaa aggatccagt caggccacct ttgctgcagc
     1801 cgtgctctat gttcacaacg aacgctggga cggcgttcct tttatcctgc gctgtggaaa
     1861 agctctgaat gagaggaaag ctgaggtgcg actgcagttc acagacgtcc ctggagacat
     1921 ttttgaaaac aaatgtcaaa ggaatgagtt ggtggtgcgt gtgcagccca atgaagccat
     1981 ctacgccaaa atgatgagca agaaaccggg agtgtacttc agccctgaag aaacagagct
     2041 ggacctcacc tacaagagca gatacaagga tgtgaaactt ccagatgctt acgagcgcct
     2101 catcctggac gtcttctgtg ggagtcagat gcatttcgtt cgcagtgatg aacttagaga
     2161 ggcatggagg atcttcaccc ctcttcttca ccacatagag gagaagaagc cacagcctat
     2221 tccttacaag tatggaagcc gtggtccagc agaagcagat gaactggttc agaaggttgg
     2281 atttcgctat gaaggcactt acaaatgggt caaccctcac aaactcgacc cagctttctt
     2341 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2401 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2461 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2521 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     2581 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     2641 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     2701 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     2761 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     2821 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     2881 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     2941 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     3001 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     3061 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     3121 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3181 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3241 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3301 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3361 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3421 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     3481 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3541 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3601 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3661 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3721 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3781 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3841 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3901 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3961 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4021 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4081 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4141 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4201 gtt
//
