LOCUS       sequence-329526-        5088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri ILF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-ILF3(2of2)
FEATURES             Location/Qualifiers
     source          1..5088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3213..3312)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3329..3348)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3330..3348)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3353..3369)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3353..3369)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3471..3490)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3482..4291
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3549..3568)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4438..5026
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4927..4946
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgcc tcctccaatg cgccaccgat caatgcgtat
      721 ctttatgaat ggagaccgcc atgtcatggc caaacactct gcggtctatc caacccagga
      781 ggagctggaa agtgttcaga acatggtgtc tcacacagag cgtgctctga aggcagtatc
      841 cgactggctg gataagcagg aaaggggaac ctgtaagtct gagtctgaat gcacagaagc
      901 cgacaaggac agtgagcaga aagatccggc cactcgcact ctgcgtggtg tcatgagggt
      961 gggtctggtt gctaagggtc tgctactgaa aggggacctg gacctggagc tggtgctact
     1021 ctgtagagac aagcctactg tcactctgct gaaaaaagtg tctgaaaacc tggtcacaca
     1081 gctcaagaca ttcactgaag acaaatatgt ggtgactcag aacatccgtg aagccagcat
     1141 catcatcaag aacaccaagg agcttcctct caccctcacc attcacctga catctcctct
     1201 ggtccgtgaa gaggtagaaa agacttctac tggagacacg ctttcagtca tcgatccccc
     1261 ggatgttctg gacaggcaca aatgcctgac agccttggca tctctacgcc acgctaagtg
     1321 gttccaggcc agagccaacg ggctgcgctc ctgtgtcatt gtcatccgga tcctgaggga
     1381 cctgtgtacc cgcgtcccca catggtctcc gctccgtggc tggccgatgg agctgatctg
     1441 tgagaaagca atcggcacag ggaaccggcc catgggggca ggcgaggcgc tacggagggt
     1501 tctggagtgt cttgcttctg gaatcctcat ggcagacggg atttatgacc cctgtgagaa
     1561 ggagcccaca gatgctatag gtcacttgga ccagcagcag agggaggaca ttactgccag
     1621 tgcacaacac gctttaaggc tgtcggcctt tggacagctc cacaaagtgc ttgggatgga
     1681 tcccctcccg tctaaaatgc ccaaaaagac tcgtaccgac actcctattg attacacggt
     1741 gcaaattcct ccgagcacca cctacgcccc gcccatgaag agacccattg aagatgagga
     1801 aggaactgat gataagagtc caaacaagaa gaagaaaaag ctgcagaaaa agtctgggga
     1861 ggagaaggct gaacctcctc aggccatgaa tgccctaatg aggctgaacc agctgaaacc
     1921 agggctccag tataagctga tctctcagac tggtccggtt catgttccgg tcttcaccat
     1981 ggccgtagag gtcgatggta agaactttga agcatctggt ccatccaagc gcaccgccaa
     2041 gcttcatgta gctgtgaagg tcctgcagga catgggcctg cccaccggag tggagctgaa
     2101 gtctgagcca acggtgaagg tggaagaggc gactgtagcg gtcaccgtgg aggagtccaa
     2161 gcctgctgta acaccgagtc agactcctgc tgctgctgca ggagccgctg ctgcagacca
     2221 cacagacgct gtggaggccg ttcgccagca gggacctatt ctgactaaac acggtaagaa
     2281 cccggtcatg gagctgaacg agaagagacg gggcctgaag tacgagctca tctccgagac
     2341 tggtggcagc cacgacaagc gcttcatcat ggaggtggag atagatggac agaagttcca
     2401 gggtactgga tccaataaga aggtggccaa ggcttatgct gctctggctg ctttggagca
     2461 gctcttccct gaaggatctg cacccgatgc taccaaaaag aaaaaggggc cttccatgca
     2521 cgctgcaggc tttggcatga tgggagaccc aggtgtaccc agaggcagag gcagaggagg
     2581 gcgaggtcgt gggcgaggca gaggctttaa taatggaggc ggttacggcc aagcaggtgg
     2641 ctttggaacg tttggctacg ggaacagcgc taactcgggc tacaattact acagcagagg
     2701 aggtgcacat ggaggaggcg gggcctcgag cagcccagca gctggtcctc ctttcagcac
     2761 agcagaagct ccgcctccgg cctacggttc atactatcag gacgacggct ccttctctgc
     2821 cccttctggc aacaaaccac ccaaaaagaa acctccaatt ccaaaagttg ggaaggcggc
     2881 acttccaggt ccaataagag gaagcacagg cgctgcagga ggctatcagc caagcagctc
     2941 actgggacag ggcctgtata accagtatgg gccgggcaaa aagaccttca accagaacca
     3001 agccggtgcc gggggttaca cttacagcac ggcctacccc agccaggtga cgggaggcgc
     3061 tggaggtaac caggactaca tctatgatgg cttcagcgcc ctgtccactt acaaccccca
     3121 gggagagaac aaccaaggct ttgggtccac ccactctcag taccagcact ccggctacag
     3181 ccgggacggc agcatgactt accagtacag agacccagct ttcttgtaca aagttggcat
     3241 tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca gtcaaaataa
     3301 aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca tggtcatagc
     3361 tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg cacaagataa
     3421 aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt
     3481 tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga
     3541 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     3601 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     3661 caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc
     3721 gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc
     3781 cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
     3841 tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa
     3901 cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga
     3961 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
     4021 gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
     4081 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat
     4141 cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc
     4201 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     4261 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     4321 gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat cccttaacgt
     4381 gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4441 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4501 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4561 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4621 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4681 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4741 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     4801 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     4861 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     4921 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4981 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5041 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
