LOCUS       sequence-329528-        7116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri MAGI2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 7116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-MAGI2(v1)
FEATURES             Location/Qualifiers
     source          1..7116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5241..5340)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(5357..5376)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5358..5376)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5381..5397)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5381..5397)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5499..5518)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             5510..6319
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5577..5596)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      6466..7054
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6955..6974
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgtc gaagagcctc aagaagaaaa accactggac
      721 caacaaagtc cacgaagcgg tgataagccg ggggaaggaa ggggagctgg ggttcgagct
      781 gaaggggggc gcggagaacg gccagttccc ctacttcggc gaggtgaagc aggggaaggg
      841 actgatccag aacggaaaac tggctcaaga tgagctgctg ctggaggtca acgacatgcc
      901 agtggccggg ctcaccaccc gggacgtgct ggccgtgatt aaacactgca aagacccggt
      961 gcgcttcaaa tgtgtcaaac aagggggggt agttgacaaa gatttgaggc actacctcaa
     1021 cctgcgcttc tccaaaggct cagtggacca cgaccaccag cagatcatca gagacaacct
     1081 ctacctccgc actgttccct gtaccaccag gcagccaaag gagggggagg tgcctggggt
     1141 ggactacagt tttgtgagcg tggagcgttt tatggaactg gagcaaagtg gagccctgtt
     1201 agagagtgga acctatgaag ataacttcta cggcacacca aaacctccag cggagccgtc
     1261 cccggcagcc cctcctctga atgtgagtga agccctgctt cccggagccc ggccgagcgc
     1321 ccagggcaag agaaagagga accagtcagt aagcaacatg gagcagcgca ccagcctgga
     1381 gccacctgag gaggaggaag aggagatccc agtcgtcaat ggcaacgggg tcgcaatcac
     1441 accagagtcg agtgagcatg aggacaagag cacagacgcc tctggggagg tagcggttga
     1501 agggtccagc caggccccag catcatccga gcctgcaact gaaggggagg aggccacaca
     1561 atctccctcc aaatccccgt ccaaagtccc ggacgtagac gtggaggagc tgggtcccct
     1621 gcctgacaac tgggagatgg cctacactga gaagggagaa gtttacttca tagatcacaa
     1681 caccaaaacc acgtcgtggc tcgatcctcg actcgcaaag aaagcaaagc cgccggagga
     1741 gtgcaaggaa gacgagctgc cgtacggctg ggagaagata gatgacccca tctacggcag
     1801 ctactatgta gaccacatca atcgaaggac tcagtttgag aatcccgttc tggaagctaa
     1861 gaggcgtctg gagcagcaaa gacagatcca aagccagggc ctctctgctc tgcctttacc
     1921 tacaatatat agagaaaagc cgttgttcac cagggatccg acccagctga agggcacctt
     1981 cctgtcaaca gccctgcaga agagcaacat gggattcggg ttcacaatta ttggaggtga
     2041 cgagcccgat gagttcctgc aagtgaagag tgtcatccca gaaggacctg ctgcccagga
     2101 cgggaaaatg gacacagggg atgttattgt ctacattaat gatatctgtg tgctcggtac
     2161 cactcacgcc gacgtcgtga agctgttcca gtctgttccc atcggccaga gcgtcaccct
     2221 tgtgctctgt cgaggatatc ctctgccctt tgacccagag gatcagagtg ctgcagcaag
     2281 cacctccctc acacccatcg gcctagaaca ccgtccactt gtggtgaatg gccgaggtag
     2341 ctatgaccca tacctggagt acctgtcttt gagctcgcag cttcctcctc aagctttggc
     2401 ccaggctgga gtctctcacc ccggagacac acacctggac ggctcgtccc tgcctcccac
     2461 cacgcccggc tcagcgtcag cacacacacc tcgtgaagat aacgtgtcaa tggcctcctc
     2521 tgggactcca ggtgttggcg gggcaactgg accaggtgca gaattgctaa ctgtcaccat
     2581 ggtgaaaggc gctgaaggat ttgggttcac tatcgccgac agtccaactg gtcagcgagt
     2641 taaacaggtg ttggagccag gctcacaggg agcatcgggg ctgatagaag gagatctgat
     2701 cctggaggtg aaccagcagc cagtagctgc agcgggacac ggacgagtgg tggagctgct
     2761 caaagactgt cctgtgggag cggaggcgac gcttctcatc cagagaggag gaacaggcca
     2821 catctctcca tggaaggcct ccaaacaggg tagcccccat actaacctat caggtcccgt
     2881 cttaccgcct ggaacgccct tcccaaatca gccccaacac cgcacatctg tgcccgactc
     2941 cacagaaggc ttcgacctca acaaacctga cccttatgat ctctacgaga agtccagggc
     3001 catctacgag agcaggcgtc ctgaatacga ggaggtggag gtgcacctgc tgagggagaa
     3061 gacagggttc ggctttcgca tcctgggggg agacgaggcc gtgcaggcta ttgttattgg
     3121 cgctataata gagaacactc ctgctgagcg tgatgggcgg ctacgacctg gggatgagct
     3181 gatttcagtg gataaaaacg tggttgctgg gaaaccgcac aaatacgtaa tagacttgat
     3241 gcactcggcc gctcgcaacg gtcaagtcag cctaactgtg aggaggagag tgcaaatgcc
     3301 aggagaccat tgtccggtga acggccgtag ccccggctcg gtgtcgactc agcacagctc
     3361 tccacgtagc gatgcagcct ctgcacctaa cccatcggct gccaccatct ctgttgtcac
     3421 ctcacctcca gaggggtctg ccgttcaaac cagtgatgtg gttataaacc gcaaggagaa
     3481 cgagggcttc ggctttgtca tcatcagctc cctgaacagg cccgagaacg ccacggtcat
     3541 cactgttccc cataaaatcg gacgcatcat tgagggaagt cctgcagaca ggtgcgggaa
     3601 actgaaggtg ggagaccgga tcctggctgt aaacggacag tccatcatca gcatgccaca
     3661 tgcagacatc gtcaagctca ttaaagatgc tggactaacg gtcacgctgc acatcattcc
     3721 agaggaaggt tcccaatctg gacccagctc agagaaacaa agccccatga cccagaaaca
     3781 cagcccccag acccagccca gtcctgtagc ccagccaggc ctaggaggca cccagtcagg
     3841 ccaaatggct cctcagccag gcctagcgtc cttgcaacca tgtcaaacag cggcccaacc
     3901 tggccaaaca ccagctctaa ttaaccaaat tgtgacaggg cctcctgcac cagcagcctc
     3961 ccagcctgcg tcagctggat cccagcagag cccggtcacc cagccctcgg gcctcccccc
     4021 acagctctac ctccatgacg gcaggtcaga ggtcaaagcc aggcaggatg ttaaaccgga
     4081 cttccgtcat cctacgttca ccgactacag gcagcctcca gttgactacc gccacccacc
     4141 ggtgactgat taccgccagc cgccaactct ggactaccga cacccgcccg gcctgctgga
     4201 cttcagacag catcctgcag cagcacagtt tcctttggga atccctccag acttccgacc
     4261 acaggactat gattatttca ccgtggagtt ggagaaaagc accaaaggtt ttggttttag
     4321 tattcgtggg ggcagagagt acagcatgga cctgtttgtg ctgcgcctcg cagaggacgg
     4381 tcctgccata cgcaatggaa ggatgagggt cggggatcag atcatcgaga ttaacggcga
     4441 cagcactcgg gacatgactc acgcccgtgc tattgaactc ataaaggcgg gaggcaggcg
     4501 ggtcaggctg ctgctgaaac gaggcactgg acaggtgcca gattatgact gtgccggccc
     4561 ctgggattcc ctttctacaa cccactctgc cctgcaggaa gtgacactgg aacctcatgt
     4621 gagtccattg ccctcatccc atccagcttc agccccagac tcccctcatc gactccatct
     4681 ggaggccaaa gtgtgcgcgg cagacaaagc tccattgctg ccagaccaca gctctgtcag
     4741 aagggccaca ggtggcaggt ccaccgagca aaaatgcatc atcagggggc agggggcccc
     4801 tcagggctct ggagaggtaa accgtgggcc tggtgaaatg cagcctcggg ttttgcctcc
     4861 caaagctgtg gtgggccggt gtaatgagag gagagagatt tgctccccct gctgtgagag
     4921 aaagggccga caaacgcaag cgaggagctc tggctcgccg tgggatccgt atgttatggt
     4981 gaatctgaat ggagcatctc tggaaggagg agatggacat gtgagcctac aggagaggct
     5041 gatgtccaga agtccattca ccagagaaga cgaaaaaaac agtggccaaa gtgggtttcg
     5101 ctgccctttg aaaaccctcg aagatcctcc tgcgaggagc gcagccacct tcccgggacc
     5161 ctggaacctc cccagctctg acaaactgcc tggcacctta aggtcctacc tccacggcga
     5221 gcaggtagaa tggatccttg acccagcttt cttgtacaaa gttggcatta taagaaagca
     5281 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     5341 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     5401 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     5461 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     5521 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     5581 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     5641 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     5701 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     5761 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     5821 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     5881 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     5941 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     6001 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     6061 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     6121 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     6181 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     6241 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     6301 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     6361 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
     6421 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6481 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6541 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6601 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6661 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6721 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6781 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6841 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6901 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6961 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7021 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7081 gttcctggcc ttttgctggc cttttgctca catgtt
//
