LOCUS       sequence-329529-        6600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri MAGI2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-MAGI2(v2)
FEATURES             Location/Qualifiers
     source          1..6600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(4725..4824)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4841..4860)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4842..4860)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4865..4881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4865..4881)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4983..5002)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4994..5803
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(5061..5080)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5950..6538
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6439..6458
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgtc gaagagcctc aagaagaaaa accactggac
      721 caacaaagtc cacgaagcgg tgataagccg ggggaaggaa ggggagctgg ggttcgagct
      781 gaaggggggc gcggagaacg gccagttccc ctacttcggc gaggtgaagc aggggaaggg
      841 actgatccag aacggaaaac tggctcaaga tgagctgctg ctggaggtca acgacatgcc
      901 agtggccggg ctcaccaccc gggacgtgct ggccgtgatt aaacactgca aagacccggt
      961 gcgcttcaaa tgtgtcaaac aagggggggt agttgacaaa gatttgaggc actacctcaa
     1021 cctgcgcttc tccaaaggct cagtggacca cgaccaccag cagatcatca gagacaacct
     1081 ctacctccgc actgttccct gtaccaccag gcagccaaag gagggggagg tgcctggggt
     1141 ggactacagt tttgtgagcg tggagcgttt tatggaactg gagcaaagtg gagccctgtt
     1201 agagagtgga acctatgaag ataacttcta cggcacacca aaacctccag cggagccgtc
     1261 cccggcagcc cctcctctga atgtgagtga agccctgctt cccggagccc ggccgagcgc
     1321 ccagggcaag agaaagagga accagtcagt aagcaacatg gagcagcgca ccagcctgga
     1381 gccacctgag gaggaggaag aggagatccc agtcgtcaat ggcaacgggg tcgcaatcac
     1441 accagagtcg agtgagcatg aggacaagag cacagacgcc tctggggagg tagcggttga
     1501 agggtccagc caggccccag catcatccga gcctgcaact gaaggggagg aggccacaca
     1561 atctccctcc aaatccccgt ccaaagtccc ggacgtagac gtggaggagc tgggtcccct
     1621 gcctgacaac tgggagatgg cctacactga gaagggagaa gtttacttca tagatcacaa
     1681 caccaaaacc acgtcgtggc tcgatcctcg actcgcaaag aaagcaaagc cgccggagga
     1741 gtgcaaggaa gacgagctgc cgtacggctg ggagaagata gatgacccca tctacggcag
     1801 ctactatgta gaccacatca atcgaaggac tcagtttgag aatcccgttc tggaagctaa
     1861 gaggcgtctg gagcagcaaa gacagatcca aagccagggc ctctctgctc tgcctttacc
     1921 tacaatatat agagaaaagc cgttgttcac cagggatccg acccagctga agggcacctt
     1981 cctgtcaaca gccctgcaga agagcaacat gggattcggg ttcacaatta ttggaggtga
     2041 cgagcccgat gagttcctgc aagtgaagag tgtcatccca gaaggacctg ctgcccagga
     2101 cgggaaaatg gacacagggg atgttattgt ctacattaat gatatctgtg tgctcggtac
     2161 cactcacgcc gacgtcgtga agctgttcca gtctgttccc atcggccaga gcgtcaccct
     2221 tgtgctctgt cgaggatatc ctctgccctt tgacccagag gatcagagtg ctgcagcaag
     2281 cacctccctc acacccatcg gcctagaaca ccgtccactt gtggtgaatg gccgaggtag
     2341 ctatgaccca tacctggagt acctgtcttt gagctcgcag cttcctcctc aagctttggc
     2401 ccaggctgga gtctctcacc ccggagacac acacctggac ggctcgtccc tgcctcccac
     2461 cacgcccggc tcagcgtcag cacacacacc tcgtgaagat aacgtgtcaa tggcctcctc
     2521 tgggactcca ggtgttggcg gggcaactgg accaggtgca gaattgctaa ctgtcaccat
     2581 ggtgaaaggc gctgaaggat ttgggttcac tatcgccgac agtccaactg gtcagcgagt
     2641 taaacaggtg ttggagccag gctcacaggg agcatcgggg ctgatagaag gagatctgat
     2701 cctggaggtg aaccagcagc cagtagctgc agcgggacac ggacgagtgg tggagctgct
     2761 caaagactgt cctgtgggag cggaggcgac gcttctcatc cagagaggag gaacaggcca
     2821 catctctcca tggaaggcct ccaaacaggg tagcccccat actaacctat caggtcccgt
     2881 cttaccgcct ggaacgccct tcccaaatca gccccaacac cgcacatctg tgcccgactc
     2941 cacagaaggc ttcgacctca acaaacctga cccttatgat ctctacgaga agtccagggc
     3001 catctacgag agcaggcgtc ctgaatacga ggaggtggag gtgcacctgc tgagggagaa
     3061 gacagggttc ggctttcgca tcctgggggg agacgaggcc gtgcaggcta ttgttattgg
     3121 cgctataata gagaacactc ctgctgagcg tgatgggcgg ctacgacctg gggatgagct
     3181 gatttcagtg gataaaaacg tggttgctgg gaaaccgcac aaatacgtaa tagacttgat
     3241 gcactcggcc gctcgcaacg gtcaagtcag cctaactgtg aggaggagag tgcaaatgcc
     3301 aggagaccat tgtccggtga acggccgtag ccccggctcg gtgtcgactc agcacagctc
     3361 tccacgtagc gatgcagcct ctgcacctaa cccatcggct gccaccatct ctgttgtcac
     3421 ctcacctcca gaggggtctg ccgttcaaac cagtgatgtg gttataaacc gcaaggagaa
     3481 cgagggcttc ggctttgtca tcatcagctc cctgaacagg cccgagaacg ccacggtcat
     3541 cactgttccc cataaaatcg gacgcatcat tgagggaagt cctgcagaca ggtgcgggaa
     3601 actgaaggtg ggagaccgga tcctggctgt aaacggacag tccatcatca gcatgccaca
     3661 tgcagacatc gtcaagctca ttaaagatgc tggactaacg gtcacgctgc acatcattcc
     3721 agaggaaggt tcccaatctg gacccagctc agagaaacaa agccccatga cccagaaaca
     3781 cagcccccag acccagccca gtcctgtagc ccagccaggc ctaggaggca cccagtcagg
     3841 ccaaatggct cctcagccag gcctagcgtc cttgcaacca tgtcaaacag cggcccaacc
     3901 tggccaaaca ccagctctaa ttaaccaaat tgtgacaggg cctcctgcac cagcagcctc
     3961 ccagcctgcg tcagctggat cccagcagag cccggtcacc cagccctcgg gcctcccccc
     4021 acagctctac ctccatgacg gcaggttaga cgacacaaag agggatgctc ttgtttggtc
     4081 agaggtcaaa gccaggcagg atgttaaacc ggacttccgt catcctacgt tcaccgacta
     4141 caggcagcct ccagttgact accgccaccc accggtgact gattaccgcc agccgccaac
     4201 tctggactac cgacacccgc ccggcctgct ggacttcaga cagcatcctg cagcagcaca
     4261 gtttcctttg ggaatccctc cagacttccg accacaggac tatgattatt tcaccgtgga
     4321 gttggagaaa agcaccaaag gttttggttt tagtattcgt gggggcagag agtacagcat
     4381 ggacctgttt gtgctgcgcc tcgcagagga cggtcctgcc atacgcaatg gaaggatgag
     4441 ggtcggggat cagatcatcg agattaacgg cgacagcact cgggacatga ctcacgcccg
     4501 tgctattgaa ctcataaagg cgggaggcag gcgggtcagg ctgctgctga aacgaggcac
     4561 tggacaggtg ccagattatg gaatggtacc gtccaacctc tccatgtgca tgaaaggtga
     4621 cacactaagc tccccctatt tcttcttaat gggccactct aaagacacga ctgtgccggc
     4681 ccctgggatt ccctttctac aacccactct gccctgcagg aaggacccag ctttcttgta
     4741 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     4801 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     4861 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     4921 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     4981 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     5041 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     5101 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     5161 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     5221 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     5281 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     5341 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     5401 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     5461 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     5521 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     5581 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     5641 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     5701 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     5761 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     5821 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     5881 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5941 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6001 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6061 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6121 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6181 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6241 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6301 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6361 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6421 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6481 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6541 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
