LOCUS       sequence-329538-       19999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For inducing predictable deletions in rice plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19999)
  AUTHORS   Wang S, Zong Y, Lin Q, Zhang H, Chai Z, Zhang D, Chen K, Qiu JL, Gao
            C
  TITLE     Precise, predictable multi-nucleotide deletions in rice and wheat 
            using APOBEC-Cas9.
  JOURNAL   Nat Biotechnol. 2020 Dec;38(12):1460-1465. doi: 
            10.1038/s41587-020-0566-4. Epub 2020 Jun 29.
  PUBMED    32601432
REFERENCE   2  (bases 1 to 19999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0566-4"; journalName: "Nat Biotechnol"; date: 
            "2020-12"; volume: "38"; issue: "12"; pages: "1460-1465"
COMMENT     Sequence Label: pH-AFID-3
FEATURES             Location/Qualifiers
     source          1..19999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1439..5539
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1439..5539
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             5452..5460
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5540..5587
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6293..6313
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6329..6385
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7172..7192
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7851..7867)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7851..7867)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7864..7886)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7875..7891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7899..7905,7906..7923,7924..7929))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7944..7965
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8156..8832
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     8769..8789
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             8899..9924
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    9965..10139
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10217..10241
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             10666..11460
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11547..12135
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12036..12055
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12289..12306
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12321..12461
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12387..12406)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12547..12569
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      12805..12999
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(13065..14138)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(14567..15196)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    16497..16521
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     16709..16731
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     16723..16740
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     16724..16740
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        16807..17186
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     CDS             17466..18257
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        18409..18484
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(18788..19999,1..780)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
ORIGIN
        1 gttccgcaga cgggatcgat ttcatgattt tttttgtttc gttgcatagg gtttggtttg
       61 cccttttcct ttatttcaat atatgccgtg cacttgtttg tcgggtcatc ttttcatgct
      121 tttttttgtc ttggttgtga tgatgtggtc tggttgggcg gtcgttctag atcggagtag
      181 aattctgttt caaactacct ggtggattta ttaattttgg atctgtatgt gtgtgccata
      241 catattcata gttacgaatt gaagatgatg gatggaaata tcgatctagg ataggtatac
      301 atgttgatgc gggttttact gatgcatata cagagatgct ttttgttcgc ttggttgtga
      361 tgatgtggtg tggttgggcg gtcgttcatt cgttctagat cggagtagaa tactgtttca
      421 aactacctgg tgtatttatt aattttggaa ctgtatgtgt gtgtcataca tcttcatagt
      481 tacgagttta agatggatgg aaatatcgat ctaggatagg tatacatgtt gatgtgggtt
      541 ttactgatgc atatacatga tggcatatgc agcatctatt catatgctct aaccttgagt
      601 acctatctat tataataaac aagtatgttt tataattatt ttgatcttga tatacttgga
      661 tgatggcata tgcagcagct atatgtggat ttttttagcc ctgccttcat acgctattta
      721 tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt gtttggtgtt acttctgcag
      781 ccctaggatg gaggccagcc cggctagcgg cccaaggcat ctcatggacc cgcacatctt
      841 caccagcaac ttcaacaacg gcatcggcag gcacaagacc tacttgtgct acgaggtgga
      901 gaggctcgac aacggaacct ccgtgaagat ggaccaacac agggggttcc tccacaacca
      961 agccaagaac ctcctctgcg gcttctacgg caggcacgcc gagttgaggt tcctcgactt
     1021 ggtgccatcc ctccaactcg atccagccca aatctaccgc gtgacctggt tcatctcctg
     1081 gtccccatgc ttctcctggg gttgcgccgg cgaggttcgg gctttcctcc aagaaaacac
     1141 ccacgtccgc ctccgcattt tcgccgccag gatctatgat tacgaccctc tctacaagga
     1201 ggccctccag atgctgcggg acgccggtgc tcaggtgagt atcatgacct acgacgagtt
     1261 caagcactgc tgggacacct tcgttgacca ccagggctgc ccattccaac catgggacgg
     1321 tctggatgaa cacagccaag ccttgtccgg caggctccgg gccatcctcc aaaaccaggg
     1381 gaactccggg agcgagacgc caggcacctc cgagtcggcc accccagaat ctaggcctga
     1441 caagaagtac tcgatcggcc tcgatattgg gactaactct gttggctggg ccgtgatcac
     1501 cgacgagtac aaggtgccct caaagaagtt caaggtcctg ggcaacaccg atcggcattc
     1561 catcaagaag aatctcattg gcgctctcct gttcgacagc ggcgagacgg ctgaggctac
     1621 gcggctcaag cgcaccgccc gcaggcggta cacgcgcagg aagaatcgca tctgctacct
     1681 gcaggagatt ttctccaacg agatggcgaa ggttgacgat tctttcttcc acaggctgga
     1741 ggagtcattc ctcgtggagg aggataagaa gcacgagcgg catccaatct tcggcaacat
     1801 tgtcgacgag gttgcctacc acgagaagta ccctacgatc taccatctgc ggaagaagct
     1861 cgtggactcc acagataagg cggacctccg cctgatctac ctcgctctgg cccacatgat
     1921 taagttcagg ggccatttcc tgatcgaggg ggatctcaac ccggacaata gcgatgttga
     1981 caagctgttc atccagctcg tgcagacgta caaccagctc ttcgaggaga accccattaa
     2041 tgcgtcaggc gtcgacgcga aggctatcct gtccgctagg ctctcgaagt ctcggcgcct
     2101 cgagaacctg atcgcccagc tgccgggcga gaagaagaac ggcctgttcg ggaatctcat
     2161 tgcgctcagc ctggggctca cgcccaactt caagtcgaat ttcgatctcg ctgaggacgc
     2221 caagctgcag ctctccaagg acacatacga cgatgacctg gataacctcc tggcccagat
     2281 cggcgatcag tacgcggacc tgttcctcgc tgccaagaat ctgtcggacg ccatcctcct
     2341 gtctgatatt ctcagggtga acaccgagat tacgaaggct ccgctctcag cctccatgat
     2401 caagcgctac gacgagcacc atcaggatct gaccctcctg aaggcgctgg tcaggcagca
     2461 gctccccgag aagtacaagg agatcttctt cgatcagtcg aagaacggct acgctgggta
     2521 cattgacggc ggggcctctc aggaggagtt ctacaagttc atcaagccga ttctggagaa
     2581 gatggacggc acggaggagc tgctggtgaa gctcaatcgc gaggacctcc tgaggaagca
     2641 gcggacattc gataacggca gcatcccaca ccagattcat ctcggggagc tgcacgctat
     2701 cctgaggagg caggaggact tctacccttt cctcaaggat aaccgcgaga agatcgagaa
     2761 gattctgact ttcaggatcc cgtactacgt cggcccactc gctaggggca actcccgctt
     2821 cgcttggatg acccgcaagt cagaggagac gatcacgccg tggaacttcg aggaggtggt
     2881 cgacaagggc gctagcgctc agtcgttcat cgagaggatg acgaatttcg acaagaacct
     2941 gccaaatgag aaggtgctcc ctaagcactc gctcctgtac gagtacttca cagtctacaa
     3001 cgagctgact aaggtgaagt atgtgaccga gggcatgagg aagccggctt tcctgtctgg
     3061 ggagcagaag aaggccatcg tggacctcct gttcaagacc aaccggaagg tcacggttaa
     3121 gcagctcaag gaggactact tcaagaagat tgagtgcttc gattcggtcg agatctctgg
     3181 cgttgaggac cgcttcaacg cctccctggg gacctaccac gatctcctga agatcattaa
     3241 ggataaggac ttcctggaca acgaggagaa tgaggatatc ctcgaggaca ttgtgctgac
     3301 actcactctg ttcgaggacc gggagatgat cgaggagcgc ctgaagactt acgcccatct
     3361 cttcgatgac aaggtcatga agcagctcaa gaggaggagg tacaccggct gggggaggct
     3421 gagcaggaag ctcatcaacg gcattcggga caagcagtcc gggaagacga tcctcgactt
     3481 cctgaagagc gatggcttcg cgaaccgcaa tttcatgcag ctgattcacg atgacagcct
     3541 cacattcaag gaggatatcc agaaggctca ggtgagcggc cagggggact cgctgcacga
     3601 gcatatcgcg aacctcgctg gctcgccagc tatcaagaag gggattctgc agaccgtgaa
     3661 ggttgtggac gagctggtga aggtcatggg caggcacaag cctgagaaca tcgtcattga
     3721 gatggcccgg gagaatcaga ccacgcagaa gggccagaag aactcacgcg agaggatgaa
     3781 gaggatcgag gagggcatta aggagctggg gtcccagatc ctcaaggagc acccggtgga
     3841 gaacacgcag ctgcagaatg agaagctcta cctgtactac ctccagaatg gccgcgatat
     3901 gtatgtggac caggagctgg atattaacag gctcagcgat tacgacgtcg atcatatcgt
     3961 tccacagtca ttcctgaagg atgactccat tgacaacaag gtcctcacca ggtcggacaa
     4021 gaaccggggc aagtctgata atgttccttc agaggaggtc gttaagaaga tgaagaacta
     4081 ctggcgccag ctcctgaatg ccaagctgat cacgcagcgg aagttcgata acctcacaaa
     4141 ggctgagagg ggcgggctct ctgagctgga caaggcgggc ttcatcaaga ggcagctggt
     4201 cgagacacgg cagatcacta agcacgttgc gcagattctc gactcacgga tgaacactaa
     4261 gtacgatgag aatgacaagc tgatccgcga ggtgaaggtc atcaccctga agtcaaagct
     4321 cgtctccgac ttcaggaagg atttccagtt ctacaaggtt cgggagatca acaattacca
     4381 ccatgcccat gacgcgtacc tgaacgcggt ggtcggcaca gctctgatca agaagtaccc
     4441 aaagctcgag agcgagttcg tgtacgggga ctacaaggtt tacgatgtga ggaagatgat
     4501 cgccaagtcg gagcaggaga ttggcaaggc taccgccaag tacttcttct actctaacat
     4561 tatgaatttc ttcaagacag agatcactct ggccaatggc gagatccgga agcgccccct
     4621 catcgagacg aacggcgaga cgggggagat cgtgtgggac aagggcaggg atttcgcgac
     4681 cgtcaggaag gttctctcca tgccacaagt gaatatcgtc aagaagacag aggtccagac
     4741 tggcgggttc tctaaggagt caattctgcc taagcggaac agcgacaagc tcatcgcccg
     4801 caagaaggac tgggatccga agaagtacgg cgggttcgac agccccactg tggcctactc
     4861 ggtcctggtt gtggcgaagg ttgagaaggg caagtccaag aagctcaaga gcgtgaagga
     4921 gctgctgggg atcacgatta tggagcgctc cagcttcgag aagaacccga tcgatttcct
     4981 ggaggcgaag ggctacaagg aggtgaagaa ggacctgatc attaagctcc ccaagtactc
     5041 actcttcgag ctggagaacg gcaggaagcg gatgctggct tccgctggcg agctgcagaa
     5101 ggggaacgag ctggctctgc cgtccaagta tgtgaacttc ctctacctgg cctcccacta
     5161 cgagaagctc aagggcagcc ccgaggacaa cgagcagaag cagctgttcg tcgagcagca
     5221 caagcattac ctcgacgaga tcattgagca gatttccgag ttctccaagc gcgtgatcct
     5281 ggccgacgcg aatctggata aggtcctctc cgcgtacaac aagcaccgcg acaagccaat
     5341 cagggagcag gctgagaata tcattcatct cttcaccctg acgaacctcg gcgcccctgc
     5401 tgctttcaag tacttcgaca caactatcga tcgcaagagg tacacaagca ctaaggaggt
     5461 cctggacgcg accctcatcc accagtcgat taccggcctc tacgagacgc gcatcgacct
     5521 gtctcagctc gggggcgaca agcggccagc ggcgacgaag aaggcggggc aggcgaagaa
     5581 gaagaagggt accgactccg gcggcagcgc caacgagctg acctggcacg acgtgctcgc
     5641 cgaggagaag caacagccat acttcctgaa caccctccaa accgtggctt ccgagaggca
     5701 gagcggcgtc accatctacc caccacaaaa ggacgtgttc aacgccttcc gcttcaccga
     5761 gctgggcgac gtcaaggtgg tcatcctcgg ccaagaccca taccacggcc caggccaggc
     5821 tcacggcctc gccttctccg tcaggccagg catcgccatc ccaccaagcc tcctgaacat
     5881 gtacaaggag ctggagaaca ccatcccagg cttcaccagg ccaaaccacg gctacctcga
     5941 gtcctgggct aggcaaggcg tgctcctgct caacaccgtg ctgaccgtca gggctggcca
     6001 ggctcactcc cacgccagcc tgggctggga gacgttcacc gacaaggtca tctccctcat
     6061 caaccagcac agggagggcg tggtgttcct cctctggggc agccacgccc aaaagaaggg
     6121 cgccatcatc gacaagcaga ggcaccacgt gctgaaggcc ccacacccgt ccccactctc
     6181 cgctcacagg ggcttcttcg gctgcaacca cttcgtgctg gccaaccaat ggctcgagca
     6241 gaggggcgag acgccaatcg actggatgcc agtcctccca gccgagagcg agccaaagaa
     6301 gaagcggaag gtgcttaagg gctccggcgc tacgaacttc tccctcctga agcaggctgg
     6361 cgacgtggag gagaacccag gcccgccgga gggcccagag atcaggcgcg ctgctgacaa
     6421 cctcgaggct gccatcaagg gcaagccact gaccgacgtg tggttcgcct tcccacaact
     6481 caagccatac caatcccagc tgatcggcca gcacgtgacc cacgtggaga cgaggggcaa
     6541 ggccctcctg acccacttct ccaacgacct gaccctctac agccacaacc aactctacgg
     6601 cgtgtggcgc gtggtggaca ccggcgagga gccacaaacc acccgcgtgc tgagggtcaa
     6661 gctccagacc gccgacaaga ccatcctcct gtactccgcc agcgacatcg agatgctgac
     6721 cccagagcaa ctcaccaccc acccattcct ccagcgcgtg ggcccagacg tcctggaccc
     6781 aaacctcacc ccagaggtgg tcaaggagag gctcctgagc ccaaggttcc gcaacaggca
     6841 attcgccggc ctcctgctgg accaggcctt cctggctggc ctcggcaact acctgcgcgt
     6901 ggagatcctc tggcaagtcg gcctgaccgg caaccacaag gccaaggacc tcaacgctgc
     6961 tcagctggac gccctcgctc acgccctgct cgagatccca cgcttctcct acgccaccag
     7021 gggccaagtg gacgagaaca agcaccacgg cgccctcttc cgcttcaagg tgttccaccg
     7081 cgacggcgag ccatgcgaga ggtgcggcag catcatcgaa aagaccaccc tgtccagcag
     7141 gccattctac tggtgcccag gctgccagca cccaaagaag aagcggaagg tgtaggagct
     7201 cagagctttc gttcgtatca tcggtttcga caacgttcgt caagttcaat gcatcagttt
     7261 cattgcgcac acaccagaat cctactgagt ttgagtatta tggcattggg aaaactgttt
     7321 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg
     7381 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt
     7441 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa
     7501 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg
     7561 aagttaatat gaggagtaaa acacttgtag ttgtaccatt atgcttattc actaggcaac
     7621 aaatatattt tcagacctag aaaagctgca aatgttactg aatacaagta tgtcctcttg
     7681 tgttttagac atttatgaac tttcctttat gtaattttcc agaatccttg tcagattcta
     7741 atcattgctt tataattata gttatactca tggatttgta gttgagtatg aaaatatttt
     7801 ttaatgcatt ttatgacttg ccaattgatt gacaacgaat tcgtaatcat gtcatagctg
     7861 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7921 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7981 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     8041 gcggggagag gcggtttgcg tattggctag agcagcttgc caacatggtg gagcacgaca
     8101 ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga
     8161 cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc
     8221 acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata
     8281 aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     8341 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     8401 gatgtgaaca tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc
     8461 tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc
     8521 ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc
     8581 acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac
     8641 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
     8701 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
     8761 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
     8821 aggacacgct gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga
     8881 tccggggggc aatgagatat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     8941 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     9001 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     9061 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     9121 ccggaagtgc ttgacattgg ggagtttagc gagagcctga cctattgcat ctcccgccgt
     9181 tcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctacaaccg
     9241 gtcgcggagg ctatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     9301 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     9361 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     9421 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     9481 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     9541 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     9601 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     9661 gagcttgcag gatcgccacg actccgggcg tatatgctcc gcattggtct tgaccaactc
     9721 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     9781 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     9841 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     9901 actcgtccga gggcaaagaa atagagtaga tgccgaccgg gatctgtcga tcgacaagct
     9961 cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag
    10021 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
    10081 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc
    10141 cccgaattaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt
    10201 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc
    10261 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg
    10321 gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg
    10381 gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg
    10441 ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc
    10501 cgtcgatact atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta
    10561 tttaaggttt tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc
    10621 ttggggtatc tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga
    10681 atatcaccgg aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga
    10741 atgtctcctg ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg
    10801 acggacagcc ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta
    10861 tggctggaag gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg
    10921 agcaatctgc tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa
    10981 caaagccctg aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc
    11041 gacatatcgg attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac
    11101 ttactgaata acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt
    11161 aaagatccgc gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc
    11221 ttttcccacg gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc
    11281 tttattgatc ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc
    11341 cggtcgatca gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg
    11401 gggatcaagc ctgattggga gaaaataaaa tattatattt tactggatga attgttttag
    11461 tacctagaat gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    11521 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    11581 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    11641 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
    11701 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    11761 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    11821 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    11881 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    11941 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    12001 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    12061 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    12121 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    12181 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    12241 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    12301 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
    12361 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
    12421 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
    12481 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
    12541 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
    12601 agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct
    12661 ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga
    12721 cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct
    12781 tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag agttttaata
    12841 agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac
    12901 atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca
    12961 atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct
    13021 ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat
    13081 gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc
    13141 tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa
    13201 cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat
    13261 ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg
    13321 ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg
    13381 atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg
    13441 ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg
    13501 ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag
    13561 gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg
    13621 tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg
    13681 ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg
    13741 gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc
    13801 acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg
    13861 cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg
    13921 cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg
    13981 cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt
    14041 tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg
    14101 ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg
    14161 ttccagtgcc attgcagggc cggcaggcaa cccagccgct tacgcctggc caaccgcccg
    14221 ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc
    14281 cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt
    14341 agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac
    14401 atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc
    14461 gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca
    14521 cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg
    14581 atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt
    14641 caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg
    14701 actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg
    14761 tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa
    14821 tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct
    14881 gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct
    14941 ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa
    15001 tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat
    15061 cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca
    15121 gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta
    15181 cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata
    15241 cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg
    15301 gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa
    15361 accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta
    15421 cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc
    15481 gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg
    15541 tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc
    15601 acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca
    15661 gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt
    15721 gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc
    15781 ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg
    15841 gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc
    15901 aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg
    15961 gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc
    16021 ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg
    16081 cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg
    16141 cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc
    16201 tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag
    16261 ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac
    16321 ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta
    16381 ctttgatccc gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa
    16441 ccttttcacg cccttttaaa tatccgatta ttctaataaa cgctcttttc tcttaggttt
    16501 acccgccaat atatcctgtc aaacactgat agtttaaact gaaggcggga aacgacaatc
    16561 tgatccaagc tcaagctgct ctagcattcg ccattcaggc tgcgcaactg ttgggaaggg
    16621 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
    16681 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
    16741 gccaagctta gtaattcatc caggtctcca agttctagga ttttcagaac tgcaacttat
    16801 tttatcaagg aatctttaaa catacgaaca gatcacttaa agttcttctg aagcaactta
    16861 aagttatcag gcatgcatgg atcttggagg aatcagatgt gcagtcaggg accatagcac
    16921 aagacaggcg tcttctactg gtgctaccag caaatgctgg aagccgggaa cactgggtac
    16981 gttggaaacc acgtgatgtg aagaagtaag ataaactgta ggagaaaagc atttcgtagt
    17041 gggccatgaa gcctttcagg acatgtattg cagtatgggc cggcccatta cgcaattgga
    17101 cgacaacaaa gactagtatt agtaccacct cggctatcca catagatcaa agctgattta
    17161 aaagagttgt gcagatgatc cgtggcgtga gaccaaccca gtggacataa gcctgttcgg
    17221 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
    17281 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
    17341 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
    17401 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
    17461 acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
    17521 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
    17581 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
    17641 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
    17701 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
    17761 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
    17821 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
    17881 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
    17941 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
    18001 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
    18061 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
    18121 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
    18181 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
    18241 aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg
    18301 cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg
    18361 tcaagtctgc ttttattatt tttaagcgtg cataataagc cggtctcggt tttagagcta
    18421 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
    18481 gtgctttttt ttttcgtttt gcattgagtt ttctccgtcg catgtttgca gttttatttt
    18541 ccgttttgca ttgaaatttc tccgtctcat gtttgcagcg tgttcaaaaa gtacgcagct
    18601 gtatttcact tatttacggc gccacatttt catgccgttt gtgccaacta tcccgagcta
    18661 gtgaatacag cttggcttca cacaacactg gtgacccgct gacctgctcg tacctcgtac
    18721 cgtcgtacgg cacagcattt ggaattaaag ggtgtgatcg atactgcttg ctgctaagct
    18781 tgcatgcctg cagtgcagcg tgacccggtc gtgcccctct ctagagataa tgagcattgc
    18841 atgtctaagt tataaaaaat taccacatat tttttttgtc acacttgttt gaagtgcagt
    18901 ttatctatct ttatacatat atttaaactt tactctacga ataatataat ctatagtact
    18961 acaataatat cagtgtttta gagaatcata taaatgaaca gttagacatg gtctaaagga
    19021 caattgagta ttttgacaac aggactctac agttttatct ttttagtgtg catgtgttct
    19081 cctttttttt tgcaaatagc ttcacctata taatacttca tccattttat tagtacatcc
    19141 atttagggtt tagggttaat ggtttttata gactaatttt tttagtacat ctattttatt
    19201 ctattttagc ctctaaatta agaaaactaa aactctattt tagttttttt atttaataat
    19261 ttagatataa aatagaataa aataaagtga ctaaaaatta aacaaatacc ctttaagaaa
    19321 ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga taatgccagc ctgttaaacg
    19381 ccgtcgacga gtctaacgga caccaaccag cgaaccagca gcgtcgcgtc gggccaagcg
    19441 aagcagacgg cacggcatct ctgtcgctgc ctctggaccc ctctcgagag ttccgctcca
    19501 ccgttggact tgctccgctg tcggcatcca gaaattgcgt ggcggagcgg cagacgtgag
    19561 ccggcacggc aggcggcctc ctcctcctct cacggcaccg gcagctacgg gggattcctt
    19621 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac
    19681 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gatctccccc
    19741 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc cccccccctc
    19801 tctaccttct ctagatcggc gttccggtcc atggttaggg cccggtagtt ctacttctgt
    19861 tcatgtttgt gttagatccg tgtttgtgtt agatccgtgc tgctagcgtt cgtacacgga
    19921 tgcgacctgt acgtcagaca cgttctgatt gctaacttgc cagtgtttct ctttggggaa
    19981 tcctgggatg gctctagcc
//
