LOCUS       sequence-329539-        4422 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri NCOA5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-NCOA5
FEATURES             Location/Qualifiers
     source          1..4422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2547..2646)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2663..2682)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2664..2682)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2687..2703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2687..2703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2805..2824)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2816..3625
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2883..2902)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3772..4360
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4261..4280
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgat gtctcacaga agaagccgca gcccatcacc
      721 agcgcgatat actcgcaggt gcagcagtaa cgatccaaga gatttggagc gaagaatttt
      781 tgttgggaat ttaccaactt ctgacatggc aaacaaggat ttggaagaaa tgttcagccc
      841 atacgggaaa gtaatcggcg tttccttgtt tcgaggatat ggatttgtac aatttgagcg
      901 cattgaggaa gctgaagctg caaaggctgg tcaaaagggt cgaatatata aaggttacaa
      961 aatagatgta aatatggcag tggaacgacg tcaagctaaa cctcaatccc agccgagtcc
     1021 tccacgaagg gccccgtaca gcaaagaatc ccgtcctcgc tcacgctcac ctgtttatgg
     1081 gcgcgacggt cgtgagcctc gagaccgtga tgcaagggat gctggtagag atccccgacc
     1141 aggaacccac tctcgggacc ctgattttcg ctaccgctca gacagccgag acaaggatgc
     1201 cagaggtgac ccacgagagc ctgctgcata tagccgggag gaatatgacc ggtacttccg
     1261 cgctggcagc ggtgcagagg actactacag gaggaaggaa gatccctaca gggatccatt
     1321 cctagatccc tggaatggac gccgtgaggc agcagaggat cgagttcgac cagaagagcg
     1381 ccggcgtaat gagctgtacc gacagtactt tgaagaactc cagcggcgct atgacgctga
     1441 tcgtcccgtt gactgctctg tgatcgtcgt caacaaggcg cagaataggg agtatgcgga
     1501 gacggtcggg cggaaagtcc gtgatttggg aatggttgtc gatctgatct tcctcaacac
     1561 agaagtgtct cttacccagg cacttgagga tgtaggccga gcccgtactc cctttgccat
     1621 catcattacc cagcagcacc aggtgcaccg ctcttgcact gttaacatcc tgtttggaac
     1681 ccctcaagag catcgaaaca tgccgatgca ggatgccatg atgctggttg cccacaactt
     1741 tgacaccttt aaagtggaaa accgtgaaaa agaacgagag gagattgcca ggaaggctgc
     1801 caaaatggca gatgacgttt tacttcggga accggacaga gagagccatc ccgtgtcggt
     1861 tctcactggc atcactctgc tggctgaaaa caggttttta actccagagg aactggacag
     1921 tctcattgca tacctgaaag acaaacgcgc tcgccttgta cgaaccccag cagatcctac
     1981 tgcagctgga gtccatgccc tacctcctgc aggagtgcat cgtgatcctc aagcccacgt
     2041 cctacatcaa gatgcttcat cagcagcagg acccgctcct ccgtctgctc atgcccctct
     2101 gcaaccaagt cacccttcag ctgttccaag ttcctcagct aaccaacagg agctgcaggc
     2161 taaaatcctc agtctgttta acagcggctc tggttcatta ccaggagctg cttcctcttc
     2221 tccccagtca cactcctaca gttctcttgg ttcgactcct ggccagaacc cctcccgccc
     2281 agctatgctc agtgcttctc cagctacagc ccagagctac ggcgcccctc caggtcgcat
     2341 gcctgtggct ccagctggtc agagaccccc tgcttcaggt atcaactttg acaacccaag
     2401 tgtccagaaa gctcttgata ctctgattca aagtgggcca tccctcaata acctggtggg
     2461 ctccagtgtc cctcagcagc cacccctaag agcagcaccc agtatgggcc aggcccctcc
     2521 catgtccatg tatcctcgtc agtacgaccc agctttcttg tacaaagttg gcattataag
     2581 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     2641 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     2701 ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     2761 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     2821 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     2881 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     2941 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     3001 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     3061 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     3121 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     3181 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     3241 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     3301 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     3361 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     3421 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     3481 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     3541 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     3601 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     3661 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta
     3721 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3781 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3841 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3901 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3961 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     4021 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     4081 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     4141 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4201 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4261 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4321 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4381 tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
