LOCUS       sequence-32954-e         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Bryant DM, Datta A, Rodriguez-Fraticelli AE, Peranen J, 
            Martin-Belmonte F, Mostov KE
  TITLE     A molecular network for de novo generation of the apical surface and
            lumen.
  JOURNAL   Nat Cell Biol. 2010 Nov;12(11):1035-45. doi: 10.1038/ncb2106. Epub 
            2010 Oct 3.
  PUBMED    20890297
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2106"; 
            journalName: "Nat Cell Biol"; date: "2010-11"; volume: "12"; issue: 
            "11"; pages: "1035-45"
COMMENT     Sequence Label: pLKO.1-puro-cfRabin8_4
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     98..117
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    268..385
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tttgactgta aacacaaaga tattaataca aagtacgtga cgtagaaagt aataatttct
       61 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
      121 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgggcc
      181 ctgtaaacac agaattctcg agaattctgt gtttacaggg ctttttgaat tctcgacctc
      241 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
      301 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
      361 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
      421 gccgcggctc gagggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg
      481 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
      541 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
      601 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcg
//
