LOCUS       sequence-329540-        4263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri NOP58.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4263)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 4263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-NOP58
FEATURES             Location/Qualifiers
     source          1..4263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2388..2487)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2504..2523)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2505..2523)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2528..2544)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2528..2544)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2646..2665)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2657..3466
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2724..2743)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3613..4201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4102..4121
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgct cgttctattt gaaaccgccg ctggatatgc
      721 gatattcaaa gtcctaaatg aatcaaagct gcagcaggtg gacagcctgt acaaggagtt
      781 tgagactcct gaaaaagcca acaaaattgt gaaactgaaa cactttgaga agtttcagga
      841 cacgacggag gcactggcag ctgccaccgc tctggttgag ggaaaaatca gcaaaagttt
      901 gaagaagatc ctgaagaaag tcgtggccaa ggaggctcat gaggagctag ccatcagcga
      961 tgctaaactg ggtggagtta ttaaagacaa gctggacctg aactgcgtct acagccccgc
     1021 agtagccgag ctgatgagat gcatcaggag ccagatggag agtctgatct ctggacttcc
     1081 tcccagagag atgagcgcca tgtctctggg cttggctcac agtttgtccc gctacaagct
     1141 gaagttcagc ccggacaaag tggacacgat gatcgttcag gccatttccc tcctggacga
     1201 cctggacaag gagcttaata actacatcat gcgctgcaga gagtggtacg gctggcactt
     1261 ccccgagctg gggaaggtcg tcacggacaa cctagcttac tgcaaaaccg tcctcaaagt
     1321 cggtgatcgt acaaacgtgt ccagcaccga tctgtcagaa atcctccctg aggagatcga
     1381 ggctgaggtg aagctagctg ctgagatctc catgggaaca gaagtatccg aacaggacat
     1441 cgacaacatc cggcacctgt gtgatcaggt gattgagatt tcagagtacc gcgctcaact
     1501 ctatgactac ttgaaaaaca ggatgatggc aatcgccccc aacctgacgg tgatggtagg
     1561 agagctggtg ggggctcggc tcatctcaca tgccgggtca ctcctgaatc tggctaagca
     1621 cccggcctcc acggtccaga tcctcggagc agagaaggct ctgttcagag cgctgaagac
     1681 ccgtaaggac acacccaagt acggtctgat ttaccacgcc tccttagtgg gtcagaccac
     1741 ggccaagaac aaaggaaaga tttccagaat gttagcagct aaagcggcgc tagctattcg
     1801 ctatgacgct ctaggagaag acaccaacgc agaaatggga gcagagaacc gagccaagct
     1861 ggaggctcgg ctgcgccacc tagaggataa aggaatcagg agaatcagtg gaacgggcaa
     1921 agcgatggcc agggcagata aatatcagca taagagtgaa gtgaggattt atgacccctc
     1981 tggggactcg accatcccgt ccacctccaa gaagaggaag attgaggagg tagaggagga
     2041 gaccccggag cccgtaacac cagtggttaa agccaaaaaa atcagaaagg agccagaaac
     2101 agaagaaact gaaacggcag cagaagccgc agagccaccc aagaaaaaga agaagaagaa
     2161 agacaaaagt gcagcagaac tggaggagcc aaaggaggag gaggaggagg tagcagtcag
     2221 tgagaccaca gggaagaaga agaagaagaa gaaagacaag agcgtaagag aactgcagga
     2281 gccaaaggag gagccaaagg aggaggagga ggaggaggta gctgtcggcg agacctcaga
     2341 aaagaagaag aagaagaaaa agaaggtgaa agaagaggaa gacgaggacc cagctttctt
     2401 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2461 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2521 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2581 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     2641 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     2701 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     2761 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     2821 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     2881 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     2941 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     3001 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     3061 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     3121 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     3181 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3241 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3301 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3361 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3421 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3481 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     3541 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3601 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3661 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3721 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3781 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3841 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3901 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3961 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4021 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4081 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4141 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4201 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4261 gtt
//
