LOCUS       sequence-32955-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Bryant DM, Datta A, Rodriguez-Fraticelli AE, Peranen J, 
            Martin-Belmonte F, Mostov KE
  TITLE     A molecular network for de novo generation of the apical surface and
            lumen.
  JOURNAL   Nat Cell Biol. 2010 Nov;12(11):1035-45. doi: 10.1038/ncb2106. Epub 
            2010 Oct 3.
  PUBMED    20890297
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2106"; 
            journalName: "Nat Cell Biol"; date: "2010-11"; volume: "12"; issue: 
            "11"; pages: "1035-45"
COMMENT     Sequence Label: pLKO.1-puro-cfRabin8_5
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    295..412
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     889..907
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
       61 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      121 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      181 cttgtggaaa ggacgaaaca ccgggctggg atatttcaaa gagctcgagc tctttgaaat
      241 atcccagctt tttgaattct cgacctcgag acaaatggca gtattcatcc acaattttaa
      301 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
      361 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
      421 ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg ttgcgccttt
      481 tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac
      541 gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga
      601 tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg
      661 aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta
      721 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg
      781 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc
      841 gggaggcggg gtgtggggcg gtagtgtggc cctgttcctg cccgcgcggt gttccgcatt
      901 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc g
//
