LOCUS       sequence-32956-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Bryant DM, Datta A, Rodriguez-Fraticelli AE, Peranen J, 
            Martin-Belmonte F, Mostov KE
  TITLE     A molecular network for de novo generation of the apical surface and
            lumen.
  JOURNAL   Nat Cell Biol. 2010 Nov;12(11):1035-45. doi: 10.1038/ncb2106. Epub 
            2010 Oct 3.
  PUBMED    20890297
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb2106"; 
            journalName: "Nat Cell Biol"; date: "2010-11"; volume: "12"; issue: 
            "11"; pages: "1035-45"
COMMENT     Sequence Label: pLKO.1-puro-cfSec15A_2
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     123..142
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    293..410
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
       61 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
      121 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      181 tgtggaaagg acgaaacacc gggaggatga gaatgaagag gctcgagcct cttcattctc
      241 atcctctttt tgaattctcg acctcgagac aaatggcagt attcatccac aattttaaaa
      301 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagccacag
      361 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
      421 acagggacag cagagatcca ctttggccgc ggctcgaggg ggttggggtt gcgccttttc
      481 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
      541 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
      601 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
      661 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
      721 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
      781 gccaatag
//
