LOCUS       sequence-329583-        2993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express 3x flag IREB2, suppressible by doxycycline addition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Terzi EM, Sviderskiy VO, Alvarez SW, Whiten GC, Possemato R
  TITLE     Iron-sulfur cluster deficiency can be sensed by IRP2 and regulates 
            iron homeostasis and sensitivity to ferroptosis independent of IRP1 
            and FBXL5.
  JOURNAL   Sci Adv. 2021 May 26;7(22). pii: 7/22/eabg4302. doi: 
            10.1126/sciadv.abg4302. Print 2021 May.
  PUBMED    34039609
REFERENCE   2  (bases 1 to 2993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2021-05-26"; volume: "7(22). pii: 7/22/eabg4302. doi"; pages:
            " 10.1126/sciadv.abg4302. Print 2021 May"
COMMENT     Sequence Label: pCW57.1 blast OFF 3xflag IREB2
FEATURES             Location/Qualifiers
     source          1..2993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..83
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 gctagcctcg agacatggac tacaaagacc atgacggtga ttataaagat catgacatcg
       61 attacaagga tgacgatgac aagatggacg ccccaaaagc aggatacgcc tttgagtacc
      121 ttattgaaac attaaatgac agttcacata agaagttctt cgatgtatct aaacttggca
      181 ccaagtatga tgttctgcct tactcaatac gggtcttgtt ggaagctgct gtacgaaatt
      241 gtgatggctt tttaatgaag aaggaagatg ttatgaacat tttagactgg aaaaccaaac
      301 aaagcaatgt tgaagtgccc tttttccctg cccgtgttct tcttcaagat tttactggaa
      361 taccagcaat ggtggatttt gctgctatga gggaggcagt gaaaactctt ggaggtgatc
      421 ctgagaaagt ccatcctgct tgtccgacag atcttacagt tgaccattct ttacaaattg
      481 acttcagtaa atgtgcaata cagaatgcac cgaacccagg cggtggtgac ctgcagaaag
      541 caggaaagct ctctccactt aaagtgcagc ctaagaagct tccctgcaga ggccagacta
      601 cctgccgagg atcttgtgat tctggagaac taggccgaaa ctcaggaaca ttttcttcgc
      661 agattgagaa tacacccatc ctgtgtcctt ttcatttgca accagtgcct gaacctgaaa
      721 cagtgttaaa aaatcaagaa gtagaattcg gcagaaatcg agagaggctt cagtttttta
      781 agtggagttc aagagttttt aagaatgtgg cagtgatccc tcctgggacc gggatggccc
      841 atcaaataaa cttagaatat ttgtcaagag tggtttttga agaaaaagac ctcctcttcc
      901 cagacagtgt agtcggcaca gattcacaca taacgatggt gaatggttta gggattctgg
      961 ggtggggggt tggaggcatt gaaacagaag cagttatgct tggtctgcca gtttctctta
     1021 ctttaccaga ggtggttgga tgtgagttaa ctgggtcatc aaaccctttt gttacatcca
     1081 tagatgttgt tcttggtatt acaaagcacc tcaggcaagt aggagtggct ggaaagtttg
     1141 ttgagttttt tggaagtgga gtttcacaat tatctatagt tgatcgaact acaatagcaa
     1201 acatgtgtcc ggaatatggt gctatcctca gctttttccc tgttgacaat gtgacattaa
     1261 aacatttaga acatacaggt tttagcaaag ccaaactcga atcaatggaa acatacctta
     1321 aagctgtgaa attgtttcga aatgaccaga attcttcagg agaacctgaa tactcccagg
     1381 tgatccagat taatctgaat tcaatagttc catctgttag tggtccaaaa agacctcagg
     1441 atagagttgc tgtgacagat atgaaaagcg atttccaggc ttgcttaaat gaaaaggttg
     1501 gatttaaagg cttccaaatt gcagctgaaa aacaaaagga tattgtctcc attcattatg
     1561 aaggaagtga atataagctg tctcatggat cagtggtcat tgctgcagtt atcagttgta
     1621 ccaataattg caatccatct gtcatgcttg ctgcaggtct tttggctaaa aaggctgttg
     1681 aagctggtct gcgtgttaaa ccttatataa gaacaagttt atctccaggc agtgggatgg
     1741 ttacacatta cctcagttca agtggagtat taccatatct aagtaagctt ggatttgaaa
     1801 tcgttggcta tggatgttca acttgtgtgg gaaatacagc acccttatca gacgcagttt
     1861 taaatgcagt aaaacagggt gatttggtta cctgtggaat tttatctgga aacaaaaatt
     1921 ttgaaggtcg tctttgtgat tgtgttcgtg ccaattatct tgcctctcca cccttagtgg
     1981 tagcttatgc catagcaggc acagtgaata tagatttcca gacagaacct ttaggtactg
     2041 accccaccgg caagaacatt tacctgcatg atatttggcc tagtcgagaa gaagttcatc
     2101 gagtagagga agaacatgtt atactatcca tgtttaaagc attaaaagat aaaatagaaa
     2161 tggggaataa acggtggaat tccttagaag caccggattc agttttgttt ccatgggact
     2221 taaagtctac ttatatcaga tgcccttcat tttttgataa acttaccaaa gagccaattg
     2281 cactccaggc tattgaaaat gcccatgtct tattatattt gggagactct gtcacaacag
     2341 atcatatatc acctgcagga agtatcgcta ggaatagtgc tgccgctaag tatttgacaa
     2401 acagaggcct tacccctcgt gaattcaact cttacggagc tcgaagaggt aatgatgctg
     2461 taatgacaag aggcactttt gcaaatatca agctttttaa taagtttatt ggaaaaccag
     2521 ctcctaaaac aattcatttt ccatcaggac agacgctaga tgtatttgag gctgcagagc
     2581 tgtaccagaa agaaggtatc ccactgatta ttttagcagg aaagaaatat ggttcaggaa
     2641 actccagaga ctgggctgcc aaaggaccgt atttactggg tgtgaaagct gttttggctg
     2701 aaagttatga aaaaatacac aaagatcatt tgattggaat tggcatagct ccacttcagt
     2761 tccttccagg agaaaatgca gattccttgg gcctctccgg tagagaaaca ttttctttaa
     2821 catttcctga agaactgtct cctggaatta cattgaatat acagacaagc actggaaaag
     2881 tattcagcgt gattgcttcg tttgaagatg atgtggaaat aacattatac aaacatggag
     2941 gattattaaa ctttgtggca cgaaaattct catagtatcg cggccgcgtc gac
//
