LOCUS       sequence-329585-        4860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri PRPF39.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-PRPF39(1of2)
FEATURES             Location/Qualifiers
     source          1..4860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2985..3084)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3101..3120)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3102..3120)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3125..3141)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3125..3141)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3243..3262)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3254..4063
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3321..3340)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4210..4798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4699..4718
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgct ggacactgag tccgctgcca tggagagcaa
      721 tggaaatgca aatcttcctg actttcctgt tgaaggtcaa gcagctgagt ggtctgcgag
      781 ccacgtttct tcagaaccac agcccgcctt gccaggagac tctgatgcat ctcgggatgc
      841 tccgggacag gaacagcaac aaatgaactg tacagagctg gcatctgtgg agaaagctgt
      901 tgagcagttt cagctggcaa atgctcagct ctttcaagaa gagcacccac cgcctccacc
      961 accgccacca ccaccccctc cgcctgcaga agaagcagaa caagcagcag aacccactgg
     1021 gtatcagcca gaggctgctg tacaggttga aagtcaagat ggggctggag aacagttgat
     1081 ggaggatggc atggagctgg aagaagcacc caaagacagt gtacaagaat ctgctgatcc
     1141 tggagaacca cagctgccct ctgagtttga aaagctctat aaagcatgtg aggatacccc
     1201 tgacgatttt aacgcctggg tcaccctgct gcagtatgtg gagcaagaaa atttccttgt
     1261 agcagcaaga aaatcatttg acatgttctt cctacgttac ccctactgct atggctactg
     1321 gaagaagtat gcagatcttg agaaaaagca tggaaatata caggtggcag aagaggtata
     1381 cagacgaggc cttcaggcca ttcccctcag tgtggatctt tggcttcact acctcacctt
     1441 tatcaaagag aactccgatc tcactgaccc agaaactgag ggacgcattc gagcctccta
     1501 cgaacacgca gtgcttgcag caggaacgga ctttcgctca gaccgcctgt gggaggcctt
     1561 cataacctgg gagaccgaac agcagaagct ggctaatgtt actgctatct atgatcgcat
     1621 cctgggcatc ccaacacagc tgtactcgca gcactttcaa aagttcaagg accacgtgca
     1681 gaacaaccac cccagatact tcctgtctga agaggagttt gttcagctta gggttgagct
     1741 ctctaaatcc agcctgtctg caatggttgg tgaagatgga gaaccgagtg ttgctcaaga
     1801 ggagctaccg cctggcactg aggatctcgc agatcctgct aagagagtaa cagagatcga
     1861 gaacatgcgc cacaaggtca ttgagagtcg ccaggaagtg ttcaaccaca acgagcatga
     1921 agtcagcaag cgctgggcct ttgaagaagg gatcaagaga ccgtacttcc acgtcaaagc
     1981 cttggagaag acgcagctga acaactggag agaatatctg gaatttgaaa tagaaaacgg
     2041 cactccggag cgtgtggttg tcctatttga acgatgcctc atcgcctgtg ctctctacga
     2101 ggagttctgg atcaagtatg caaaatatct agagggcttc agcaccgatg gagtgagaca
     2161 tgtcttcaag aaggcgtgca ccatccacct ccccaagaaa ccctccatcc acgtgatgtg
     2221 ggcggccttt gaggagcagc aaggcaacat cgaagaggct cagaggatcc tgaaattcct
     2281 ggaagaagcg atcccgggtc tggctctggt gcgtcttcgg agggttagtc tggagcgtcg
     2341 ccgcgggaac atggagggag cagaagcctt gttgagggaa gcgatggagt ccgcgaagag
     2401 ttctgttgag atgtcgttct atgctgtgaa gctggctcga catctaatta aagtgcagag
     2461 gagtctgagc aaggcgaaaa aggtgctgct cgaggctatc gagaaggatc agacaagttc
     2521 aaagctgtat ttgaatctac tggagctgga gtttagtgaa ggtgtccagc agaacgaagc
     2581 ggagatcttg gcctgttttg accgagcctt gaggagctcc atgcccctgg aatcccgcat
     2641 caccttttcc cagcgcaagg tggaatttct ggaagacttt ggcagtgaca tcaacgtgct
     2701 tgtggcggca tacgaggaac aacaaaagct acagaaagaa agcgaatctt tgaagaggaa
     2761 ggctgagaat ggttatgaca gctctcaaga aagtgacgcc aaaagacagc gtgtggacga
     2821 cccttcagct gttgcggcaa acgccatgac agacatgcag gcaaacagct ctgcgtacaa
     2881 ctacaactgg taccagcaac agtacggtgg ctggggacag aattcctggg gacagtacaa
     2941 ccagtatgcc cagtacaacc aatactaccc tccccctcct acagacccag ctttcttgta
     3001 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     3061 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     3121 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     3181 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     3241 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     3301 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     3361 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     3421 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     3481 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     3541 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     3601 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     3661 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     3721 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     3781 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     3841 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     3901 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     3961 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     4021 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     4081 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     4141 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4201 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4261 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4321 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4381 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4441 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4501 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4561 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4621 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4681 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4741 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4801 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
