LOCUS       sequence-329596-        4125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri SCARB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-SCARB2(v1)
FEATURES             Location/Qualifiers
     source          1..4125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2250..2349)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2366..2385)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2367..2385)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2390..2406)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2390..2406)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2508..2527)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2519..3328
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2586..2605)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3475..4063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3964..3983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgat gagtcgaaga cccttcgcca tctacacggt
      721 gggggccctc tgcgccctcc tgctggtctg tgggatcggt ctgatcctgg ggcaagtttt
      781 tcgcacgctc atgcacaatc gcctgaaaaa ggagattgtt ttagttgagg gcagccgtgt
      841 gttcgagtca tggaagaggc ctcctcctcc tgttttcatg gaatatttct tcttcaatgt
      901 cactaatgtt gatgagatcc tgaaaggggc caaaccgatt gtcactcagg tcggcccgta
      961 cacatacagg gagtacaggt ataaagacaa tgtgaccatg gtggaaaatg gaacaatggt
     1021 gtctgcatac aacacaaaaa gctttgtgtt cctgctagag aagtctgtgg gagacccaac
     1081 tgtcgacaac atcactaccg tgaacattcc tgcttgggct gcgatgaaca aggcaaaaat
     1141 cagtttcttt aggagctcca tgttaaacat gtggatgaac tcattaagga ctggtttatt
     1201 caccacccgc actgtacatg aactactttg gggttatgaa gattcactgc tggtcaaaat
     1261 ctccaccacc aatcgtgaag tggaaaagga ctttggccta atgatcaaga aaaatggaag
     1321 taatgacgga gaatttgtct tccacactgg agaacagaac tacttggatt atggccgagt
     1381 taaaacatgg aaaggtcaaa gacagctgac tttctggtca accaatgagt caaacagcat
     1441 cgctggatca gatgggagtg ctttccatcc cctgctgaac aaggatgaac gcatttatat
     1501 tttcactcca gatctctgca ggacaatcta catggatttt gagaaggatg tggtggtgaa
     1561 aggaatccca gcataccgtt tcacaccacc gagctccgtt ttggccagca aggaggagaa
     1621 cccagccaac gaagggttct gtgtcacctc aaatgagtgc ctgggaaccg gggtcctaaa
     1681 agtcagcccc tgtcggaaag gtgcccccgt ggtggcctcc tttcctcatt tctacctggg
     1741 ggaagacaag tacatagatg ccattggagg aatgtcccct caaagggagc accatcaaac
     1801 ctttctggac ctgaacccga ccacgggaat aatcgtacgt gcaaacaaaa gagcacaagt
     1861 caacatcttg gttggcagag tcgttggatt cccgaaaact aaaaacctga ctgagaccat
     1921 tttccctgtt atgttcctca atgagagtgt ggagattgat gatgcatctg caatgagggt
     1981 gcagaagctg ctgctgatca ctaaactcgt gtccaacttc ccactgttca ttgtgggcct
     2041 gggtggcatc atgctgatga tcttcatcgt cctcctgttt cgagctcata aacaaaagaa
     2101 ggaagttaag cgcattgagt ttaccaagcc ttactgtgct acttccactg aagaagacag
     2161 ctcctactct ccagtcagcc aaaaggacaa ggacgacccc caaaacggga actgcatcgg
     2221 cttgacgcct aaagggggca ctcaggccga cccagctttc ttgtacaaag ttggcattat
     2281 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     2341 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     2401 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     2461 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     2521 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     2581 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     2641 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     2701 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     2761 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     2821 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     2881 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     2941 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     3001 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     3061 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     3121 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     3181 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     3241 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     3301 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     3361 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     3421 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3481 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3541 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3601 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     3661 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3721 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3781 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3841 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3901 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3961 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4021 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4081 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
