LOCUS       sequence-329597-        4083 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri SCARB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-SCARB2(v2)
FEATURES             Location/Qualifiers
     source          1..4083
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2208..2307)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2324..2343)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2325..2343)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2348..2364)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2348..2364)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2466..2485)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2477..3286
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2544..2563)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3433..4021
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3922..3941
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgat gagtcgaaga cccttcgcca tctacacggt
      721 gggggccctc tgcgccctcc tgctggtctg tgggatcggt ctgatcctgg ggcaagtttt
      781 tcgcacgctc atgcacaatc gcctgaaaaa ggagattgtt ttagttgagg gcagccgtgt
      841 gttcgagtca tggaagaggc ctcctcctcc tgttttcatg gaatatttct tcttcaatgt
      901 cactaatgtt gatgagatcc tgaaaggggc caaaccgatt gtcactcagg tcggcccgta
      961 cacatacagg gagtacaggt ataaagacaa tgtgaccatg gtggaaaatg gaacaatggt
     1021 gtctgcatac aacacaaaaa gctttgtgtt cctgctagag aagtctgtgg gagacccaac
     1081 tgtcgacaac atcactaccg tgaacattcc tgcttgggct gcgatgaaca aggcaaaaat
     1141 cagtttcttt aggagctcca tgttaaacat gtggatgaac tcattaagga ctggtttatt
     1201 caccacccgc actgtacatg aactactttg gggttatgaa gattcactgc tggtcaaaat
     1261 ctccaccacc aatcgtgaag tggaaaagga ctttggccta atgatcaaga aaaatggaag
     1321 taatgacgga gaatttgtct tccacactgg agaacagaac tacttggatt atggccgagt
     1381 taaaacatgg aaaggtcaaa gacagctgac tttctggtca accaatgagt caaacagcat
     1441 cgctggatca gatgggagtg ctttccatcc cctgctgaac aaggatgaac gcatttatat
     1501 tttcactcca gatctctgca ggacaatcta catggatttt gagaaggatg tggtggtgaa
     1561 aggaatccca gcataccgtt tcacaccacc gagctccgtt ttggccagca aggaggagaa
     1621 cccagccaac gaagggttct gtgtcacctc aaatgagtgc ctgggaaccg gggtcctaaa
     1681 agtcagcccc tgtcggaaag gtgcccccgt ggtggcctcc tttcctcatt tctacctggg
     1741 ggaagacaag tacatagatg ccattggagg aatgtcccct caaagggagc accatcaaac
     1801 ctttctggac ctgaacccga ccacgggaat aatcgtacgt gcaaacaaaa gagcacaagt
     1861 caacatcttg gttggcagag tcgttggatt cccgaaaact aaaaacctga ctgagaccat
     1921 tttccctgtt atgttcctca atgagagtgt ggagattgat gatgcatctg caatgagggt
     1981 gcagaagctg ctgctgatca ctaaactcgt gtccaacttc ccactgttca ttgtgggcct
     2041 gggtggcatc atgctgatga tcttcatcgt cctcctgttt cgagctcata aacaaaagac
     2101 ttccactgaa gaagacagct cctactctcc agtcagccaa aaggacaagg acgaccccca
     2161 aaacgggaac tgcatcggct tgacgcctaa agggggcact caggccgacc cagctttctt
     2221 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2281 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2341 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2401 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     2461 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     2521 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     2581 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     2641 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     2701 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     2761 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     2821 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     2881 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     2941 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     3001 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3061 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3121 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3181 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3241 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3301 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     3361 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3421 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3481 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3541 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3601 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3661 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3721 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3781 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3841 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3901 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3961 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4021 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4081 gtt
//
