LOCUS       sequence-329598-        4260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri SF3B2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-SF3B2
FEATURES             Location/Qualifiers
     source          1..4260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2385..2484)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2501..2520)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2502..2520)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2525..2541)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2525..2541)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2643..2662)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2654..3463
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2721..2740)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3610..4198
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4099..4118
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatggc atccgacgga gtaacgggta gcgattctct
      721 ttcgcctgat ttaggaggtg caatcgcagc tttaaacaca tggagcaatc cggagcttca
      781 agccaagctg gctgacctgg gcgcaccaaa tatgggtccc agagaggagc tgattgacag
      841 actgaagggc tacatgatgc agacaggaat tatcctcagc aaaccaaatg aagacaaccc
      901 aatgggcgtc cagatgccag ggttaccttc tcttccccca ttacccatgc ctccaatgcc
      961 ccctggcatc ggaatgctcc agcctatggg catgatgccc gctggaggtc ctcctcctcc
     1021 tggtttaccc ttaggaatgg agcacggacc cctgctgcct ccagcgctga cgcaggacga
     1081 ccagctgaag atggctcagc acagggcggc catggttctc caacaggagg aacgagccaa
     1141 acaggctgct gtgctgctgg aacaggagcg tcagcaggag atggccaagc tgcagcccag
     1201 gtctttgccc attgtttcta tcttggatcc tcgtgggccg cttcctcctg gtgtcacgtt
     1261 gttgccctcc cagaaacaga gggtgccccc ccctccagga gaagacaaag aggcctggca
     1321 gcatgaagag atcagcatca gtgggccaaa gatccctcag gttctggaga agatcctgca
     1381 gctgaaggag atcagacagg ggcagctcac tgatgtagca gacgacgacg atgatgggag
     1441 ggacatggat gccaccaatt cctcagcgct gatgtctgag aacgacgaag atggtctgat
     1501 gtctaaaaaa gataaaaacc gcaagaggag gaaccgcaaa aagaagagca agaagaagcg
     1561 agctcaggag aagaaggaac aagctgagca gcagcagaag aagggaggtg gtgagaagga
     1621 gaaggagaaa gagaaaggga aggagccaga ggtggagatc gagtacgtca cagatgagcc
     1681 ggagatctac gacccgaact ttatcttctt caagaggatt tttgaggctt ttaagctgac
     1741 tgatgatgtg aaaaaagaga aggagaagga gccggagaag gcggaaaagc aggaagtttt
     1801 aggcgttctg cgaaagaaag gatttgagtt ggagaggaga gacagtgatg acagcgatga
     1861 ggaagtcaaa caggatgttc ctaaactgtc caagaagaag ctgaggagga tgaacaggct
     1921 gacagtggct gagctcaaac agctggtggc ccgacccgat gtggtggaga tgcacgacgt
     1981 gacggctcag gagcccaagc tgctggtgca cctgaaggcc accaggaaca cggtccctgt
     2041 gccgcgccac tggtgcttca aacgcaagta cctgcagggc aagcgaggca tagagaagcc
     2101 cccgttccag ctgccggagt tcatcaggag gacggggatc caggagatga gggaggctct
     2161 gcaggagaag gaggacgcca aaaccatgaa aaccaaaatg agagagaagg ttcgtcccaa
     2221 gatgggaaag atcgacatcg actaccagaa gctccacgac gcgttcttca agtggcagat
     2281 caagcccaag ctcaccatcc atggagacct ctactacgag gtatgggacc actcctctgg
     2341 ctgctcctca cttaaaaatc atctcatggc gtgtttttgt tttgacccag ctttcttgta
     2401 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     2461 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     2521 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     2581 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     2641 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     2701 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     2761 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     2821 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     2881 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     2941 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     3001 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     3061 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     3121 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     3181 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     3241 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     3301 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     3361 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     3421 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     3481 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     3541 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3601 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3661 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3721 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3781 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3841 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3901 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3961 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4021 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4081 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4141 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4201 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
