LOCUS       sequence-329601-        7198 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7198)
  AUTHORS   Kerns JA, Emerman M, Malik HS
  TITLE     Positive selection and increased antiviral activity associated with 
            the PARP-containing isoform of human zinc-finger antiviral protein.
  JOURNAL   PLoS Genet. 2008 Jan;4(1):e21. doi: 10.1371/journal.pgen.0040021.
  PUBMED    18225958
REFERENCE   2  (bases 1 to 7198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2008-01"; volume: "4(1):e21. doi"; pages: " 
            10.1371/journal.pgen.0040021"
COMMENT     Sequence Label: pcDNA4 huZAP(S)
FEATURES             Location/Qualifiers
     source          1..7198
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             724..750
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2887..2916
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2932..2949
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2972..2989)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2978..3202
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3248..3676
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3335..3354)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3545..3566
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3685..3705)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3690..4019
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3870..4005
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3932..3951
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4067..4114
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4133..4507
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4637..4758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4674..4693)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4728..4747
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4807..4823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4807..4823)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4820..4842)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4831..4847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4855..4861,4862..4879,4880..4885))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4900..4921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5038..5055)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5209..5797)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5289..5308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5968..6759,6760..6828))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6591..6610
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6829..6933)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7008..7027)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga attcgccacc
      721 atgtatccat acgatgttcc agattacgct gcggacccgg aggtgtgctg cttcatcacc
      781 aaaatcctgt gcgcccacgg gggccgcatg gccctggacg cgctgctcca ggagatcgcg
      841 ctgtctgagc cgcagctctg tgaggtgctg caggtggccg ggcccgaccg ctttgtggtg
      901 ttggagaccg gcggcgaggc cgggatcacc cgatcggtgg tggccaccac tcgagcccgg
      961 gtctgccgtc gcaagtactg ccagagaccc tgcgataacc tgcatctctg caaactcaac
     1021 ttgctgggcc ggtgcaacta ttcgcagtcc gagcggaatt tatgcaaata ttctcatgag
     1081 gttctctcag aagagaactt caaagtcctg aaaaatcacg aactctctgg actgaacaaa
     1141 gaggaattag cagtgctcct cctccaaagt gatccttttt ttatgcccga gatatgcaaa
     1201 agttataagg gagagggtcg gcagcagatt tgtaaccagc agccaccgtg ttcaagactc
     1261 cacatctgtg accacttcac ccgagggaac tgtcgttttc ccaactgcct ccggtcccat
     1321 aacctgatgg acagaaaggt gctggccatc acgagggagc acgggctgaa ccccgacgtg
     1381 gtccagaaca tccaggacat ctgcaacagc aagcacatgc agaagaatcc cccagggccc
     1441 agagctcctt cttcacatcg tagaaacatg gcatataggg ctagaagcaa gagtagagat
     1501 cggttctttc agggcagcca agaatttctt gcgtctgctt cagcgtctgc tgagaggtcc
     1561 tgcacaccta gtccagatca gatcagccac agggcttccc tggaggacgc gcctgtggac
     1621 gatctcaccc gcaagttcac gtatctgggg agtcaggatc gcgctcggcc tccctcaggc
     1681 tcgtccaagg ctactgatct tggaggaaca agtcaggccg ggacaagcca gaggttttta
     1741 gagaacggca gtcaagagga cctcttgcat ggaaatccag gcagcactta ccttgcttcc
     1801 aattcaacat cagcccccaa ctggaagagc ctcacatcct ggacgaatga ccaaggcgcc
     1861 aggagaaaga ctgtgttttc tcccacgcta cctgccgccc gctcttcttt tggctctctg
     1921 caaacacctg aagctgtgac caccagaaag ggcacaggct tgctttcctc agactacagg
     1981 atcatcaatg gcaaaagtgg aactcaggac atccagcctg gccctctttt taataataat
     2041 gctgatggag tggccacaga tataacttct accagatcct taaattacaa aagcactagc
     2101 agcggtcaca gagaaatatc atcacctagg attcaggatg ctggacctgc ttcccgagat
     2161 gtccaggcca ctggcagaat cgcagatgat gctgacccaa gagtagcact tgttaacgat
     2221 tctttatctg atgtcacaag taccacatct tctagggtgg atgatcatga ctcagaggaa
     2281 atttgtcttg accatctgtg taagggttgt ccgcttaatg gtagctgcag caaagtccac
     2341 ttccatctgc cttaccggtg gcagatgctt attggtaaaa cctggacgga ctttgagcac
     2401 atggagacga tcgagaaagg ctactgtaac cccggaatcc acctctgttc tgtaggaagt
     2461 tatacaatca attttcgggt aatgagttgt gattcctttc ccatccgacg cctctccact
     2521 ccttcttctg tcaccaagcc agccaattct gtcttcacca ccaaatggat ttggtattgg
     2581 aagaatgaat ctggcacatg gattcagtat ggagaagaga aagacaaacg gaaaaattca
     2641 aacgtcgact cttcatacct ggagtctctc tatcaatcct gtccgagggg agttgtgcca
     2701 tttcaggcgg gctcacggaa ctatgagctg agtttccaag ggatgattca gacaaacata
     2761 gcttccaaaa ctcaaaagga tgtcatcaga agaccaacat ttgtgcctca gtggtatgtg
     2821 cagcagatga agagatagga tatccagcac agtggcggcc gctcgagtct agagggcccg
     2881 cggttcgaac aaaaactcat ctcagaagag gatctgaata tgcataccgg tcatcatcac
     2941 catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     3001 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3061 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3121 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3181 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
     3241 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3301 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3361 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3421 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     3481 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3541 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     3601 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     3661 aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3721 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3781 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3841 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3901 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg
     3961 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     4021 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc
     4081 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa
     4141 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     4201 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     4261 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     4321 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     4381 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     4441 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     4501 ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     4561 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     4621 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     4681 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     4741 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta
     4801 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4861 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4921 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     4981 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     5041 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5101 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5161 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5221 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5281 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5341 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5401 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     5461 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     5521 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     5581 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     5641 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     5701 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5761 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5821 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5881 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5941 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6001 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6061 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6121 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6181 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6241 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     6301 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6361 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     6421 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     6481 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     6541 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     6601 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     6661 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     6721 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     6781 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     6841 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     6901 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     6961 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     7021 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     7081 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     7141 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgtt
//
