LOCUS       sequence-329631-        8877 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens 
            at a single cell resolution (using cytometry)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8877)
  AUTHORS   Wroblewska A, Dhainaut M, Ben-Zvi B, Rose SA, Park ES, Amir ED, 
            Bektesevic A, Baccarini A, Merad M, Rahman AH, Brown BD
  TITLE     Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens.
  JOURNAL   Cell. 2018 Nov 1;175(4):1141-1155.e16. doi: 
            10.1016/j.cell.2018.09.022. Epub 2018 Oct 18.
  PUBMED    30343902
REFERENCE   2  (bases 1 to 8877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.09.022"; journalName: "Cell"; date: "2018-11-1-
            1"; volume: "175"; issue: "4"; pages: "1141-1155.e16"
COMMENT     Sequence Label: pLKO_Tag.C.S.Ollas_NGFR
FEATURES             Location/Qualifiers
     source          1..8877
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        50..298
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     50..70
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     221..240
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        317..392
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    440..557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        697..1875
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          928..1866
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1823..1843
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1924..1933
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2026..2061
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             2080..2124
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             2143..2184
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     misc_feature    2950..3538
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3003..3023)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3421..3432)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3613..3846
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3918..4039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3955..3974)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4009..4028
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4079..4214
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4234..4253)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4235..4253)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4263..4280)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4263..4279)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4272..4294)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4421..4876
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4508..4527)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4718..4739
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4902..5006
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5007..5075,5076..5867)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5225..5244)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6038..6626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6527..6546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6780..6797
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6914..6935
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6950..6955,6956..6973,6974..6980)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6988..7004
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6993..7015
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7012..7028
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7012..7028
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7047..7067
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7049..7067
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7095..7321
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7322..7502
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7549..7674
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8167..8400
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8585..8629
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tcgacggtat cgatcacgag actagtatcg agcggccgcc cccttcaccg agggcctatt
       61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat
      121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
      301 gtcttcgaga agacctgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
      361 atcaacttga aaaagtggca ccgagtcggt gcttttttta attctcgacc tcgagacaaa
      421 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
      481 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
      541 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggccgcggc
      601 tcgaggcgat aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc
      661 taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca
      721 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
      781 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
      841 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
      901 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
      961 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
     1021 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     1081 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     1141 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     1201 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     1261 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     1321 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     1381 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     1441 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     1501 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     1561 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     1621 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     1681 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     1741 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     1801 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     1861 tttcaggtgt cgtgaggaat tctgcagtcg atcgacggta ccgcgggccc gggatccacg
     1921 gtcgccacca tgggggcagg tgccaccggc cgcgccatgg acgggccgcg cctgctgctg
     1981 ttgctgcttc tgggggtgtc ccttggaggt gccaaggagg catgcgagga ccaggtggac
     2041 cccaggctga tcgacggcaa gggaggaggc gggggaggga aggagaccgc cgccgccaag
     2101 ttcgagaggc agcacatgga cagcggagga ggcgggggag ggagcggctt cgccaacgag
     2161 ctgggcccca ggctgatggg caagtgcccc acaggcctgt acacacacag cggtgagtgc
     2221 tgcaaagcct gcaacctggg cgagggtgtg gcccagcctt gtggagccaa ccagaccgtg
     2281 tgtgagccct gcctggacag cgtgacgttc tccgacgtgg tgagcgcgac cgagccgtgc
     2341 aagccgtgca ccgagtgcgt ggggctccag agcatgtcgg cgccgtgcgt ggaggccgac
     2401 gacgccgtgt gccgctgcgc ctacggctac taccaggatg agacgactgg gcgctgcgag
     2461 gcgtgccgcg tgtgcgaggc gggctcgggc ctcgtgttct cctgccagga caagcagaac
     2521 accgtgtgcg aggagtgccc cgacggcacg tattccgacg aggccaacca cgtggacccg
     2581 tgcctgccct gcaccgtgtg cgaggacacc gagcgccagc tccgcgagtg cacacgctgg
     2641 gccgacgccg agtgcgagga gatccctggc cgttggatta cacggtccac acccccagag
     2701 ggctcggaca gcacagcccc cagcacccag gagcctgagg cacctccaga acaagacctc
     2761 atagccagca cggtggcagg tgtggtgacc acagtgatgg gcagctccca gcccgtggtg
     2821 acccgaggca ccaccgacaa cctcatccct gtctattgct ccatcctggc tgctgtggtt
     2881 gtgggccttg tggcctacat agccttcaag aggtggaaca ggggtatcct ctagagcggc
     2941 cgcgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3001 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3061 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3121 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3181 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3241 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3301 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     3361 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     3421 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     3481 ccgcatcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     3541 gggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     3601 aaaagggggg actggaaggg ctaattcact cccaacgaag ataagatctg ctttttgctt
     3661 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     3721 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     3781 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     3841 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     3901 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3961 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     4021 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     4081 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4141 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4201 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     4261 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     4321 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     4381 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     4441 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     4501 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     4561 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     4621 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     4681 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     4741 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     4801 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     4861 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4921 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4981 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5041 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5101 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5161 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5221 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5281 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5341 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5401 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5461 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5521 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5581 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5641 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5701 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5761 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5821 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5881 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5941 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6001 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6061 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6121 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6181 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6241 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6301 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6361 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6421 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6481 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6541 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6601 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6661 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6721 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6781 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     6841 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     6901 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     6961 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     7021 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     7081 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     7141 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatggcgat tggtggaagt
     7201 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     7261 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     7321 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7381 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7441 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7501 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     7561 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     7621 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     7681 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     7741 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     7801 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     7861 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     7921 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     7981 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     8041 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     8101 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     8161 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     8221 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     8281 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     8341 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     8401 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     8461 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     8521 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     8581 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     8641 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     8701 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     8761 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     8821 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatc
//
