LOCUS       sequence-329651-       10206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of mutated LbCas12aD156R using CL7/Im7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Nguyen LT, Gurijala J, Rananaware SR, Pizzano BLM, Stone BT, Jain PK
  TITLE     CRISPR-ENHANCE: An enhanced nucleic acid detection platform using 
            Cas12a.
  JOURNAL   Methods. 2021 Feb 9. pii: S1046-2023(21)00025-6. doi: 
            10.1016/j.ymeth.2021.02.001.
  PUBMED    33577982
REFERENCE   2  (bases 1 to 10206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods. 
            2021 Feb 9. pii: S1046-2023(21)00025-6. doi: 
            10.1016/j.ymeth.2021.02.001."
COMMENT     Sequence Label: CL7-LbCas12aD156R
FEATURES             Location/Qualifiers
     source          1..10206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(24..1106)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1068..1087
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1107..1184)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1390..1409
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1493..1512
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1493..1511
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1512..1536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1580..1906
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYSGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             2321..2344
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2369..2392
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             2396..2419
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             6458..6484
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6464..6490
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             6503..6523
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6530..6550
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6563..6580
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6633..6651)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6647..6694
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6731..7186
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6818..6837)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7028..7049
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7278..8093)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7278..8093)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8001..8020
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8085..8104
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8215..8803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8704..8723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8957..8974
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8989..9128
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9054..9073)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9214..9236
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9230..9421)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
       61 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg
      121 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc
      181 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg
      241 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc
      301 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
      361 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg
      421 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt
      481 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt
      541 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc
      601 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca
      661 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca
      721 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc
      781 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt
      841 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg
      901 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg
      961 ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg
     1021 tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg
     1081 acatcgtata acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc
     1141 tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc
     1201 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     1261 cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
     1321 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     1381 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     1441 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
     1501 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac
     1561 cttaagaagg agatatacca tgagcgataa aattattcac ctgactgacg acagttttga
     1621 cacggatgta ctcaaagcgg acggggcgat cctcgtcgat ttctgggcag agtggtgtgg
     1681 tccgtgcaaa atgatcgccc cgattctgga tgaaatcgct gacgaatatt ctggcaaact
     1741 gaccgttgca aaactgaaca tcgatcaaaa ccctggcact gcgccgaaat atggcatccg
     1801 tggtatcccg actctgctgc tgttcaaaaa cggtgaagtg gcggcaacca aagtgggtgc
     1861 actgtctaaa ggtcagttga aagagttcct cgacgctaac ctggcaggta gtggtacgaa
     1921 ttctgaaccg ggtgaagcaa ctggtgaggg tgaaccagtt aacaacaaat ggctggataa
     1981 cgcaggtaaa gatctgggtt ctccagttcc tgatcgcatt gctgataagc tgcgcgatca
     2041 agaatttgaa tcctttgacg atttccgtga aaccttttgg gaagaagtct ctaaagaccc
     2101 ggaactgagc aaacagttct cccgcaacaa caacgaccgc atgaaagtgg gcaaagctcc
     2161 gaaaacccgt actcaagacg tttctggcaa acgcactagc tttgaactga accatgagaa
     2221 accgattgaa gaaaacggcg gtgtttatga tatggacaac atctccgtgg ttacgccgaa
     2281 acgtaacatc gatatcgaaa accatggtga caatacgtct ctggaagtac tgtttcaagg
     2341 tcctagttct aacacctctt ctaacggctt agaggttctg tttcagggtc caggtcatca
     2401 tcaccatcac catcatcaca acgagacgtc tggttctctg caagattctg aagttaatca
     2461 ggaagctaaa ccggaagtaa aaccagaggt taaaccagaa actcacatta acctgaaagt
     2521 gagcgatggt tcttccgaaa tctttttcaa gattaaaaag actactccgc tgcgtcgtct
     2581 gatggaagca ttcgcgaaac gccaaggtaa agaaatggat tctctgcgct ttctgtatga
     2641 cggcattcgc attcaggctg accaagcacc tgaggatctg gacatggaag acaatgacat
     2701 tatcgaagct catcgtgaac agattggtgg ttctggtacc ctgcctgaaa ctggttctag
     2761 cgaagcttcc atgagcaaac tggagaagtt cacgaactgt tattctctgt cgaaaacctt
     2821 acgttttaag gccattccgg taggtaaaac gcaagaaaat atcgacaata aacgtctcct
     2881 ggttgaagat gaaaagcgtg ccgaagatta taaaggtgtt aaaaagctgc ttgatcgcta
     2941 ttacctgtct tttatcaacg atgttctgca ttccatcaaa ttgaaaaact tgaataatta
     3001 catctcgtta tttcgcaaaa aaacccgtac cgaaaaagaa aacaaggagc tggaaaacct
     3061 tgaaattaat ctgcgtaaag aaatcgcgaa agcatttaaa ggtaatgaag gatataaatc
     3121 tctgtttaaa aaagacatta ttgaaaccat cctgcctgag ttcttggatg ataaagatga
     3181 aattgccctg gttaatagct ttaatggctt tacgactgca tttaccggct tttttcgtaa
     3241 ccgtgaaaac atgttcagcg aagaagcgaa atcgacttcc attgccttcc gttgcattaa
     3301 tgaaaacctg acccgctaca tttctaatat ggacatcttt gaaaaagttg atgccatttt
     3361 cgacaaacat gaagttcaag aaatcaaaga aaagatttta aactccgatt atgatgttga
     3421 agatttcttt gagggagaat ttttcaattt tgttctcacc caagagggta ttgatgtcta
     3481 taacgccatt atcggaggat ttgttacgga atcaggcgaa aagattaaag gactgaatga
     3541 atacattaat ttatacaatc agaaaaccaa acagaaattg ccaaaattca aaccactgta
     3601 taaacaggta ttaagcgacc gtgaatcctt gtccttttat ggcgaaggct atacaagcga
     3661 tgaagaggtg ctcgaagtat tccgtaatac tttgaacaaa aactcggaaa tcttctccag
     3721 cattaaaaaa ctggaaaaat tgtttaaaaa tttcgatgaa tatagtagcg caggcatctt
     3781 cgtcaaaaat ggccctgcga tttcaaccat ttctaaagac attttcggtg aatggaacgt
     3841 gatccgcgat aaatggaacg cggagtatga cgacattcat ctgaaaaaaa aagccgttgt
     3901 taccgaaaag tatgaagatg accgccgcaa gagctttaaa aaaattggta gtttctcact
     3961 ggaacagtta caagaatatg cagacgccga tctgtcggtt gtggaaaagt taaaagagat
     4021 tattatccag aaagtcgatg aaatttataa agtgtacggc tccagcgaaa agctctttga
     4081 tgccgacttt gttttagaaa aatcgctgaa aaaaaacgat gccgtggtgg ccatcatgaa
     4141 agatctgttg gattcggtca aatcgttcga aaactatatc aaggcgttct ttggcgaagg
     4201 gaaagagacc aatcgtgacg aatcgttcta tggcgatttt gtgttggcgt atgatattct
     4261 tctgaaagta gatcacatct atgatgctat ccgcaactac gtgacccaga aaccgtattc
     4321 gaaggataaa tttaagctgt attttcaaaa ccctcagttt atgggtggct gggataaaga
     4381 taaagaaacc gactaccgcg cgacgatctt gcgctatggc tctaagtact atttagccat
     4441 catggataaa aagtatgcaa aatgcctcca gaaaatcgat aaggatgatg tcaacgggaa
     4501 ctacgaaaaa atcaactaca aactcttgcc tggcccgaat aaaatgctgc cgaaagtctt
     4561 tttttccaaa aaatggatgg cctattataa cccttccgaa gatattcaga agatttacaa
     4621 gaacggtacc tttaaaaaag gtgacatgtt taatctgaat gactgtcaca agcttattga
     4681 tttcttcaaa gatagtattt ctcgctaccc taaatggtca aacgcgtacg attttaattt
     4741 ctcagaaaca gagaaataca aagatattgc ggggttttat cgcgaagtcg aagaacaagg
     4801 gtataaggtg tcgttcgaat cggcttccaa aaaggaagtg gataaacttg ttgaagaagg
     4861 caaactctat atgttccaga tctacaacaa agatttctcc gacaagagcc atggcacgcc
     4921 gaacttacat accatgtact ttaaattact gttcgatgaa aataatcatg gtcagattcg
     4981 cttgagcggt ggcgccgaac tgtttatgcg ccgcgcatca ttgaaaaagg aagaacttgt
     5041 tgtgcaccca gcaaacagtc cgattgcaaa caaaaatccg gataacccga agaagactac
     5101 caccttaagc tatgatgtat ataaagataa acgttttagc gaagatcagt atgaactcca
     5161 cattccgatc gcgattaaca aatgtcctaa gaacatcttt aaaatcaata ccgaggttcg
     5221 tgtactgctc aaacacgacg acaaccccta tgttattggc attgaccgcg gcgaacgcaa
     5281 cctgctgtat attgtcgttg tggatggcaa aggtaacatt gttgaacagt atagccttaa
     5341 tgaaatcatc aataatttca acggcattcg tattaaaacg gactatcata gcctgctgga
     5401 caaaaaagag aaagaacgct tcgaagcccg tcagaattgg acctctatcg aaaatatcaa
     5461 ggaactgaaa gctgggtaca tttctcaagt ggtccacaag atttgcgaac tggtggaaaa
     5521 gtatgacgcg gtgatcgcac tggaggatct gaattcgggc tttaaaaata gccgtgtgaa
     5581 ggtggagaaa caggtttacc aaaagttcga aaaaatgctg attgacaaac ttaattacat
     5641 ggtggacaaa aaatctaatc catgtgcgac cggcggggct ctcaaaggct atcagattac
     5701 gaataaattc gaaagcttca aatccatgtc tacccagaac ggatttatct tttacatccc
     5761 cgcctggctg acttccaaaa ttgatccgag taccggtttt gttaatctgc tgaaaaccaa
     5821 atatacgtcg atcgccgatt caaaaaaatt cattagcagc ttcgaccgta ttatgtacgt
     5881 cccggaagaa gacctgttcg aattcgccct tgactataaa aatttctcgc gtacggatgc
     5941 cgattatatc aaaaaatgga aactgtacag ctacggcaac cgcatccgca tttttcgcaa
     6001 cccaaagaag aacaacgtat ttgactggga ggaagtttgc ttgacgagcg cttacaaaga
     6061 gctctttaac aaatatggga tcaattatca gcaaggggac atccgtgcgt tgctgtgtga
     6121 gcagtcagat aaagcgttct acagctcttt catggcttta atgtcgctga tgttgcagat
     6181 gcgcaattcg atcactggcc gcaccgatgt ggatttcctg attagcccgg tcaaaaatag
     6241 cgatggcatt ttttacgatt cacgtaacta tgaagcccag gaaaacgcta ttctgccgaa
     6301 gaacgctgat gcgaacggcg cgtacaatat tgcccgtaag gtactgtggg caattggtca
     6361 gttcaagaaa gcggaagatg aaaagttgga taaggtcaaa attgcgatct cgaataaaga
     6421 atggctggaa tatgcgcaga ccagcgtgaa acatgcctat ccctatgacg tgcccgatta
     6481 tgccagcctg ggcagcggct cccccaagaa aaaacgcaag gtggaagatc ctaagaaaaa
     6541 gcggaaagtg gactaactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
     6601 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     6661 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt
     6721 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6781 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6841 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6901 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6961 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     7021 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     7081 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     7141 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     7201 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7261 cgctcatgaa ttaattctta gaaaaactca tcgagcatca aatgaaactg caatttattc
     7321 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     7381 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     7441 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     7501 tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag
     7561 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     7621 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     7681 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     7741 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
     7801 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     7861 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     7921 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     7981 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     8041 ttggaattta atcgcggcct agagcaagac gtttcccgtt gaatatggct cataacaccc
     8101 cttgtattac tgtttatgta agcagacagt tttattgttc atgaccaaaa tcccttaacg
     8161 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8221 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8281 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8341 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     8401 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8461 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8521 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8581 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8641 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8701 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8761 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8821 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8881 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8941 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     9001 ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc
     9061 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     9121 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     9181 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     9241 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa
     9301 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg
     9361 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca
     9421 ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag
     9481 agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg
     9541 agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat
     9601 gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga
     9661 tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa
     9721 cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt
     9781 cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc
     9841 agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggg gccgccatgc
     9901 cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt
     9961 gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc
    10021 agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt
    10081 gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga
    10141 aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag
    10201 tgagct
//
