LOCUS       sequence-329668-        4193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expressed, chimeric transcription activator 
            comprising the AraC DNA binding domain and the UreR urea ligand 
            binding domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Picard HR, Schwingen KS, Green LM, Shis DL, Egan SM, Bennett MR, 
            Swint-Kruse L
  TITLE     Allosteric regulation within the highly interconnected structural 
            scaffold of AraC/XylS homologs tolerates a wide range of amino acid 
            changes.
  JOURNAL   Proteins. 2021 Aug 8. doi: 10.1002/prot.26206.
  PUBMED    34369028
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2021 Aug 8. doi: 10.1002/prot.26206."
COMMENT     Sequence Label: UUUUA-pHG165c
FEATURES             Location/Qualifiers
     source          1..4193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(314..1105,1106..1174))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     937..956
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1175..1279)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1347..1365
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     1518..1540
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1532..1549
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1533..1549
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3129..3320
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(3314..3336)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3422..3562
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3477..3496
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3577..3594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(3748..4193,1..143))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3828..3847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
       61 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      121 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      181 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      241 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      301 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      361 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      421 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
      481 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
      541 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
      601 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
      661 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
      721 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
      781 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
      841 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
      901 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
      961 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1021 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1081 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1141 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1201 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1261 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1321 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
     1381 aatccgttaa cggcattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     1441 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     1501 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagcttgg
     1561 ctgcaggtcg acggatcccc gggaattcca ccatcgaatg gtgacaccat cgaatggtgc
     1621 aaaacctttc gcggtatggc atgatagcgc ccggggccca agttcactta aaaaggagat
     1681 caacaatgaa gcaattttcg tactgaaaca tcttaatcat gctggggagg gtttctaatg
     1741 gaatataaat tctttctttc agaaagccag atggtgttaa aagcgtttta tattgaaagc
     1801 gcgatgatag ccatgctaac tggcgcgaaa ggaaatatcg tgatcaatgg ccaatccatt
     1861 gaaattgaaa gcgatgtcac cttaattatt cctaaataca gccaagtttc ctgcaatatt
     1921 gtgtgtaata ccgtgaagca gccgcttgag ttgcacacgt tgtcgatgtc agcagaagaa
     1981 ttacaagcgg tatttttatt actgaaaaca ttgatgaagt ccggtgcgcc tctgactagc
     2041 caccaaccca tctaccatat ttctccccca gaaactgtga gagataactt tagtttgcta
     2101 aaacaatgct taccattaaa aaaacagagt gcatcccaag aagcattgtt aatgaaacag
     2161 agcctatatt ttattttaat ggcgatatat caagaaggta tcgatattct gaatcttttt
     2221 cgtttcaatt acgatgaaga taatcgggta cgcgaggctt gtcagtacat cagcgatcac
     2281 ctggcagaca gcaattttga tatcgccagc gtcgcacagc atgtttgctt gtcgccgtcg
     2341 cgtctgtcac atcttttccg ccagcagtta gggattagcg tcttaagctg gcgcgaggac
     2401 caacgtatca gccaggcgaa gctgcttttg agcaccaccc ggatgcctat cgccaccgtc
     2461 ggtcgcaatg ttggttttga cgatcaactc tatttctcgc gggtatttaa aaaatgcacc
     2521 ggggccagcc cgagcgagtt ccgtgccggt tgtgaagaaa aagtgaatga tgtagccgtc
     2581 aagttgtcat aatgagaatt cgtaataata tagctctaga ttaattgcgc ccgttaacgg
     2641 cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc
     2701 ggctaggctg gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa
     2761 cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg
     2821 gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc
     2881 ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga
     2941 agcgctggca ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt
     3001 gtttaccctc acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga
     3061 gcatcctctc tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg
     3121 aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc
     3181 agacattaac gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct
     3241 gtgaatcgct tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg
     3301 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     3361 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     3421 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
     3481 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     3541 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3601 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3661 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3721 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3781 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3841 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3901 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3961 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     4021 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     4081 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     4141 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cag
//
