LOCUS       sequence-329670-        4262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expressed, chimeric transcription activator 
            comprising the AraC DNA binding domain and the MelR L-melibiose 
            ligand binding domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Picard HR, Schwingen KS, Green LM, Shis DL, Egan SM, Bennett MR, 
            Swint-Kruse L
  TITLE     Allosteric regulation within the highly interconnected structural 
            scaffold of AraC/XylS homologs tolerates a wide range of amino acid 
            changes.
  JOURNAL   Proteins. 2021 Aug 8. doi: 10.1002/prot.26206.
  PUBMED    34369028
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2021 Aug 8. doi: 10.1002/prot.26206."
COMMENT     Sequence Label: MMMMA-pHG165c
FEATURES             Location/Qualifiers
     source          1..4262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(314..1105,1106..1174))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     937..956
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1175..1279)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1347..1365
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     1518..1540
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1532..1549
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1533..1549
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3198..3389
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(3383..3405)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3491..3631
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3546..3565
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3646..3663)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(3817..4262,1..143))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3897..3916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
       61 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      121 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      181 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      241 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      301 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      361 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      421 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
      481 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
      541 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
      601 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
      661 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
      721 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
      781 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
      841 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
      901 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
      961 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1021 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1081 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1141 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1201 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1261 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1321 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
     1381 aatccgttaa cggcattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     1441 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     1501 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagcttgg
     1561 ctgcaggtcg acggatcccc gggaattcca ccatcgaatg gtgacaccat cgaatggtgc
     1621 aaaacctttc gcggtatggc atgatagcgc ccggggccca agttcactta aaaaggagat
     1681 caacaatgaa gcaattttcg tactgaaaca tcttaatcat gctggggagg gtttctaatg
     1741 aatacagata cgtttatgtg cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg
     1801 tattcggaat accagcggat ggagattgag tttcgcgcac cgcatatcat gcccaccagc
     1861 cactggcatg gtcaggtcga agtgaatgtg cctttcgatg gcgatgtgga atacctgatc
     1921 aacaatgaaa aagtgaatat caatcagggt catatcacgc tgttctgggc ctgtacaccg
     1981 caccaactaa cagataccgg aacctgtcag agcatggcga tttttaatct gccgatgcat
     2041 ctgtttctct cctggccgct ggataaagac ctgattaacc acgtcactca cggcatggtg
     2101 atcaaatcac tggccacaca gcaacttagc ccgtttgaag tgcgccgctg gcagcaggaa
     2161 ttaaacagtc cgaacgagca aattcgccag ctcgccattg atgaaattgg cctgatgctc
     2221 aagcgattta gcctctctgg ctgggaaccg attctggtca ataaaacctc gcgcacacac
     2281 aaaaacagcg tctcgcgcca tgcgcaagat aatcgggtac gcgaggcttg tcagtacatc
     2341 agcgatcacc tggcagacag caattttgat atcgccagcg tcgcacagca tgtttgcttg
     2401 tcgccgtcgc gtctgtcaca tcttttccgc cagcagttag ggattagcgt cttaagctgg
     2461 cgcgaggacc aacgtatcag ccaggcgaag ctgcttttga gcaccacccg gatgcctatc
     2521 gccaccgtcg gtcgcaatgt tggttttgac gatcaactct atttctcgcg ggtatttaaa
     2581 aaatgcaccg gggccagccc gagcgagttc cgtgccggtt gtgaagaaaa agtgaatgat
     2641 gtagccgtca agttgtcata atgagaattc gtaataatat agctctagat taattgcgcc
     2701 cgttaacggc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
     2761 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
     2821 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
     2881 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca
     2941 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg
     3001 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata
     3061 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc
     3121 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc
     3181 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
     3241 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
     3301 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
     3361 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     3421 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     3481 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     3541 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     3601 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     3661 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     3721 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     3781 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     3841 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     3901 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     3961 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4021 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4081 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4141 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4201 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     4261 ag
//
