LOCUS       sequence-329676-        4325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expressed , mutationally-enhanced chimeric 
            transcription activator comprising the AraC DNA binding domain and 
            the XylS benzoate ligand binding domain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Picard HR, Schwingen KS, Green LM, Shis DL, Egan SM, Bennett MR, 
            Swint-Kruse L
  TITLE     Allosteric regulation within the highly interconnected structural 
            scaffold of AraC/XylS homologs tolerates a wide range of amino acid 
            changes.
  JOURNAL   Proteins. 2021 Aug 8. doi: 10.1002/prot.26206.
  PUBMED    34369028
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2021 Aug 8. doi: 10.1002/prot.26206."
COMMENT     Sequence Label: XXXXA_Q182A_R287H-pHG165c
FEATURES             Location/Qualifiers
     source          1..4325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(314..1105,1106..1174))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     937..956
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1175..1279)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1347..1365
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     1518..1540
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1532..1549
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1533..1549
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3261..3452
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(3446..3468)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3554..3694
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3609..3628
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3709..3726)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(3880..4325,1..143))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3960..3979)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
       61 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      121 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      181 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      241 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      301 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      361 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      421 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
      481 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
      541 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
      601 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
      661 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
      721 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
      781 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
      841 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
      901 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
      961 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1021 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1081 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1141 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1201 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1261 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1321 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
     1381 aatccgttaa cggcattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     1441 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     1501 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagcttgg
     1561 ctgcaggtcg acggatcccc gggaattcca ccatcgaatg gtgacaccat cgaatggtgc
     1621 aaaacctttc gcggtatggc atgatagcgc ccggggccca agttcactta aaaaggagat
     1681 caacaatgaa gcaattttcg tactgaaaca tcttaatcat gctggggagg gtttctaatg
     1741 gatttttgcc tgctgaacga aaaaagccag atttttgtgc atgcggaacc gtatgcggtg
     1801 agcgattatg tgaaccagta tgtgggcacc catagcattc gcctgccgaa aggcggccgc
     1861 ccggcgggcc gcctgcatca tcgcattttt ggctgcctgg atctgtgccg cattagctat
     1921 ggcggcagcg tgcgcgtgat tagcccgggc ctggaaacct gctatcatct gcagattatt
     1981 ctgaaaggcc attgcctgtg gcgcggccat ggccaggaac attattttgc gccgggcgaa
     2041 ctgctgctgc tgaacccgga tgatcaggcg gatctgacct atagcgaaga ttgcgaaaaa
     2101 tttattgtga aactgccgag cgtggtgctg gatcgcgcgt gcagcgataa caactggcat
     2161 aaaccgcgcg aaggcattcg ctttgcggcg cgccataacc tgcagcagct ggatggcttt
     2221 attaacctgc tgggcctggt gtgcgatgaa gcggaacata ccaaaagcat gccgcgcgtg
     2281 gctgaacatt atgcgggcat tattgcgagc aaactgctgg aaatgctggg cagcaacgtg
     2341 agccgcgaaa tttttagcaa aggcaacccg gataatcggg tacgcgaggc ttgtcagtac
     2401 atcagcgatc acctggcaga cagcaatttt gatatcgcca gcgtcgcaca gcatgtttgc
     2461 ttgtcgccgt cgcgtctgtc acatcttttc cgccagcagt tagggattag cgtcttaagc
     2521 tggcgcgagg accaacgtat cagccaggcg aagctgcttt tgagcaccac ccggatgcct
     2581 atcgccaccg tcggtcacaa tgttggtttt gacgatcaac tctatttctc gcgggtattt
     2641 aaaaaatgca ccggggccag cccgagcgag ttccgtgccg gttgtgaaga aaaagtgaat
     2701 gatgtagccg tcaagttgtc ataatgagaa ttcgtaataa tatagctcta gattaattgc
     2761 gcccgttaac ggcgggcagc gttgggtcct ggccacgggt gcgcatgatc gtgctcctgt
     2821 cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga
     2881 tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat
     2941 gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca
     3001 ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga acacctacat
     3061 ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc cgccgcatcc
     3121 ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca tcatcagtaa
     3181 cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg aacagaaatc
     3241 ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac atggcccgct
     3301 ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac gcggatgaac
     3361 aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc agctgcctcg
     3421 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     3481 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     3541 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     3601 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     3661 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     3721 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3781 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3841 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3901 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3961 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4021 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4081 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4141 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4201 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4261 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4321 gacag
//
