LOCUS       sequence-32968-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J.
  TITLE     Dual RMCE for efficient re-engineering of mouse mutant alleles
  JOURNAL   Nat Methods. 2010 Oct 17
  PUBMED    20953177
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Oct 17"
COMMENT     Sequence Label: pDIRE
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     43..59
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     44..60
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        85..584
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(120..143)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     517..536
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      594..603
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             606..626
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aatgatacct actaagggac aaagctggta ccgggccccc cctcgaggtc gacggtatcg
       61 ataagcttga tatcgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag
      121 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
      181 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
      241 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
      301 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
      361 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
      421 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
      481 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
      541 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgaccatc gccgccacca
      601 tggctcctaa gaagaagagg aaggtgatga gccagttcga catcctgtgc aagacccccc
      661 ccaaggtgct ggtgcggcag ttcgtggaga gattcgagag gcccagcggc gagaagatcg
      721 ccagctgtgc cgccgagctg acctacctgt gctggatgat cacccacaac ggcaccgcca
      781 tcaagagggc caccttcatg agctacaaca ccatcatcag caacagcctg agcttcgaca
      841 tcgtgaa
//
