LOCUS       sequence-329681-        4217 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutively expressed, wild-type E coli AraC transcription 
            activator; activated by L-Arabinose..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Picard HR, Schwingen KS, Green LM, Shis DL, Egan SM, Bennett MR, 
            Swint-Kruse L
  TITLE     Allosteric regulation within the highly interconnected structural 
            scaffold of AraC/XylS homologs tolerates a wide range of amino acid 
            changes.
  JOURNAL   Proteins. 2021 Aug 8. doi: 10.1002/prot.26206.
  PUBMED    34369028
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2021 Aug 8. doi: 10.1002/prot.26206."
COMMENT     Sequence Label: AraC-pHG165c WT
FEATURES             Location/Qualifiers
     source          1..4217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(314..1105,1106..1174))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     937..956
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1175..1279)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1347..1365
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     1518..1540
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1533..1549
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1738..2616
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             3153..3344
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(3338..3360)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3446..3586
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3501..3520
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3601..3618)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(3772..4217,1..143))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3852..3871)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
       61 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      121 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      181 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      241 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      301 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      361 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      421 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
      481 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
      541 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
      601 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
      661 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
      721 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
      781 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
      841 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
      901 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
      961 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1021 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1081 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1141 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1201 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1261 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1321 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag
     1381 aatccgttaa cggcattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     1441 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     1501 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagcttgg
     1561 ctgcaggtcg acggatcccc gggaattcca ccatcgaatg gtgacaccat cgaatggtgc
     1621 aaaacctttc gcggtatggc atgatagcgc ccggggccca agttcactta aaaaggagat
     1681 caacaatgaa gcaattttcg tactgaaaca tcttaatcat gctggggagg gtttctaatg
     1741 gctgaagcgc aaaatgatcc cctgctgccg ggatactcgt ttaatgccca tctggtggcg
     1801 ggtttaacgc cgattgaggc caacggttat ctcgattttt ttatcgaccg accgctggga
     1861 atgaaaggtt atattctcaa tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga
     1921 cgagaatttg tttgccgacc gggtgatatt ttgctgttcc cgccaggaga gattcatcac
     1981 tacggtcgtc atccggaggc tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc
     2041 gcctactggc atgaatggct taactggccg tcaatatttg ccaatacggg gttctttcgc
     2101 ccggatgaag cgcaccagcc gcatttcagc gacctgtttg ggcaaatcat taacgccggg
     2161 caaggggaag ggcgctattc ggagctgctg gcgataaatc tgcttgagca attgttactg
     2221 cggcgcatgg aagcgattaa cgagtcgctc catccaccga tggataatcg ggtacgcgag
     2281 gcttgtcagt acatcagcga tcacctggca gacagcaatt ttgatatcgc cagcgtcgca
     2341 cagcatgttt gcttgtcgcc gtcgcgtctg tcacatcttt tccgccagca gttagggatt
     2401 agcgtcttaa gctggcgcga ggaccaacgt atcagccagg cgaagctgct tttgagcacc
     2461 acccggatgc ctatcgccac cgtcggtcgc aatgttggtt ttgacgatca actctatttc
     2521 tcgcgggtat ttaaaaaatg caccggggcc agcccgagcg agttccgtgc cggttgtgaa
     2581 gaaaaagtga atgatgtagc cgtcaagttg tcataatgag aattcgtaat aatatagctc
     2641 tagattaatt gcgcccgtta acggcgggca gcgttgggtc ctggccacgg gtgcgcatga
     2701 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga
     2761 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct
     2821 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt
     2881 cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg
     2941 gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt
     3001 cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt
     3061 catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca
     3121 tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa accgccctta
     3181 acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc aacgagctgg
     3241 acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat gagctttacc
     3301 gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     3361 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     3421 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     3481 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     3541 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     3601 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3661 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3721 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3781 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3841 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3901 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3961 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4021 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4081 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4141 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4201 ctacactaga aggacag
//
