LOCUS       sequence-32969-e         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J.
  TITLE     Dual RMCE for efficient re-engineering of mouse mutant alleles
  JOURNAL   Nat Methods. 2010 Oct 17
  PUBMED    20953177
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Oct 17"
COMMENT     Sequence Label: pDRAV-1
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(41..74)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(81..114)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 ttaaccctca ctaaagggaa caaaagctgg taccaagctt gaagttccta tactttctag
       61 agaataggaa cttcatcgat ataacttcgt ataggatact ttatacgaag ttatgtcgac
      121 gaccctacgc ccccaactga gagaactcaa aggttacccc agttggggca cacgcgtaac
      181 aatactgaga atgaagtgta tgtggaacag aggctgctga tctcgttctt caggctatga
      241 aactgacaca tttggaaacc acagtactta gaaccacaaa gtgggaatca agagaaaaac
      301 aatgatccca cgagagatct atagatcatg agtgggagga atgagctggc ccttaatttg
      361 gttttgcttg tttaaattat gatatccaac tatgaaacat tatcataaag caatagtaaa
      421 gagccttcag taaagagcag gcatttatct aatcccaccc cacccccacc cccgtagctc
      481 caatccttcc attcaaaatg taggtactct gttctcaccc ttcttaacaa agtatgacag
      541 gaaaaacttc cattttagtg gacatcttta ttgtttaata gatcatcaat ttctcgacta
      601 ttcctttgcc ctcggacgag tgctggggcg tcggtttcca ctatcggcga gtacttctac
      661 acagccatcg gtccagacgg ccgcgcttct gcgggcgatt tgtgtacgcc cgacagtccc
      721 ggctccggat cggacgattg cgtcgcatcg accctgcgcc caagctgcat catcgaaatt
      781 gccgtcaacc aagctctgat agagttggtc aagaccaatg cggagcatat acgcccggag
      841 ccgcggcgat cctgcaagct c
//
