LOCUS       sequence-329707-        1065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tol2 construct: actinb (actb2) promoter driven zebrafish 
            gain-of-function kcnj13 mutant, M135R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, 
            Mittal SK, Zhang G
  TITLE     Potassium Channel-Associated Bioelectricity of the Dermomyotome 
            Determines Fin Patterning in Zebrafish.
  JOURNAL   Genetics. 2020 Aug;215(4):1067-1084. doi: 
            10.1534/genetics.120.303390. Epub 2020 Jun 16.
  PUBMED    32546498
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.120.303390"; journalName: "Genetics"; date: 
            "2020-08"; volume: "215"; issue: "4"; pages: "1067-1084"
COMMENT     Sequence Label: pDestTol2-actinb-kcnj13-M135R-EGFP
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    27..51
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcca ctttgtacaa gaaagctggg tcggcgcgcc
       61 cacccttctt ctccacgcgg tcgctgcctt ctccattcag ctgaaccacc acatgctctt
      121 tatccggtgt atcagtggtg tgtgtgttgg ctagtgtgag gggacagggc accgtgtcaa
      181 aatagcgcat gtttggggtt ttgttctggt gtacagacgt caccttggag aagcagtagc
      241 catattggat ctcgtcaggc aggtaagacg tcctcttgtg gtaaccagac cctgtgcttt
      301 cctgagtcgc agtgagaaac actacaagct caaagtgtgt ttggctgctt gggttgttga
      361 tgaaaggagt ggatggattt aaagggtgaa agaaggtcag aggggagagg aaaagagggc
      421 aagatctgga gcccaggttg tcgatgctga actccagagc agtttggtgg agttcgtgat
      481 cgtctcgctc ctcgtagagg acagcactca cggatacgtc cactatcggc tgtggctgga
      541 ggttacacac tccaaacatt anacagcgct ggcctttctg ctcgcacacc acagcctgag
      601 ggctgaatag gatcccgtca catcgcttct gaggcccana gaacttggct acaaatgcac
      661 canngataag ggcctccagc atgantccca nnnncatctg canggcaagc nnctcgatgg
      721 nagtggggca gtctgcnttt gggtancntg tgccataccc aatagtgagt tgcctttggg
      781 taccnngtgn cntacccant antgagttgc gtttgggtan nntgtgncnt acccaatann
      841 gagntnnnnc tncagaggcn aatggnnaan ccngtgnnga accctttnac atgnttaaca
      901 tacnnnccat gaccgggang gangagaatc tgattcanga catccngttc nncnntgacc
      961 ctanccagca gnaccntaaa acngnnacag canncgnnga annaggaatg ancncaaaat
     1021 gcnnaaanna ncnncctnnn ncnntanngc cnncttgngc ccnna
//
