LOCUS       sequence-329708-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 construct: actinb (actb2) promoter driven zebrafish 
            gain-of-function kcnj13 mutant, M135R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Silic MR, Wu Q, Kim BH, Golling G, Chen KH, Freitas R, Chubykin AA, 
            Mittal SK, Zhang G
  TITLE     Potassium Channel-Associated Bioelectricity of the Dermomyotome 
            Determines Fin Patterning in Zebrafish.
  JOURNAL   Genetics. 2020 Aug;215(4):1067-1084. doi: 
            10.1534/genetics.120.303390. Epub 2020 Jun 16.
  PUBMED    32546498
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.120.303390"; journalName: "Genetics"; date: 
            "2020-08"; volume: "215"; issue: "4"; pages: "1067-1084"
COMMENT     Sequence Label: pDestTol2-actinb-kcnj13-M135R-EGFP
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    286..310
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tnnttctttt tactgagccc tacgactctg gctgagtgcc acaccgccgg cagccgcaaa
       61 gcggctcaaa ccattgcctt ttatggtaat aatgagagaa tgcagaggga cttcctttgt
      121 ctggcatatc tgaggcgcgc gctgtcacta gcgcccacca gcggtcagac tgtagaatgc
      181 agagcaaaac aggaagttga ctccagatgg tcacatgctt gctgaaacgc ctctccctgg
      241 cagcagtgca attctaacag tttccctttc ttttacagtt cagcctcaag tttgtacaaa
      301 aaagcaggct ccgcggccgc ccccttcacc atgcctacca ccatgacaaa caccaccgct
      361 gaccaaaagg cctcctgccc tttgatggtc aaacctcagc gtcgccgcct ggtcagcaaa
      421 gacggtcgga gccagacaag aggaaacaca agaggaggct ccagagagac gtgtttcagt
      481 gctctgcggg acttgtgggg cacatggctg gcgctacggt ggcggtgggt ggttttggca
      541 ttttgtggct cattcctgct tcactggctg ctgtttgctg tattatggta cctgctggct
      601 cgggtcaatg gagacctgga tgtcctggat catgattctc ctcctcccgg tcatgtgctg
      661 tgtgttaaac atgtgaatgg gttcacagcc gctttttcat tcgccctgga gacgcaactc
      721 actattgggt atggcacacg gtacccaaac gcaactcact attgggtatg gcacacggta
      781 cccaaacgca actcactatt gggtatggca cacggtaccc aaacgcagac tgccccactg
      841 ccatcgcgct gcttgccctg cagatgctgc tgggactcat gctggaggcc tttatcactg
      901 gtgcatttgt agccaagttc tctcngnctc nnaagcgatg tgacgggntc ctattcannc
      961 tcnnntgtgn gtgtgagcag aaaggccagc gctgtctaan gtttcgagtg ngtancncag
     1021 cncnncncta gtggacgtat ccntgagtgc
//
