LOCUS       sequence-329725-       10534 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of Zebra Finch SAP30L fused to the C-terminal 
            end of eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Biegler MT, Fedrigo O, Collier P, Mountcastle J, Haase B, Tilgner 
            HU, Jarvis ED
  TITLE     Induction of an immortalized songbird cell line allows for gene 
            characterization and knockout by CRISPR-Cas9.
  JOURNAL   Sci Rep. 2022 Mar 14;12(1):4369. doi: 10.1038/s41598-022-07434-7.
  PUBMED    35288582
REFERENCE   2  (bases 1 to 10534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2022-03-14"; volume: "12(1):4369. doi"; pages: " 
            10.1038/s41598-022-07434-7"
COMMENT     Sequence Label: pMB204: UbC-GFP-Tg_SAP30L-WPRE
FEATURES             Location/Qualifiers
     source          1..10534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(55..74)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        246..625
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        627..825
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     780..800
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             843..1023
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1070..1195
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1688..1921
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2106..2150
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2448..2565
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2657..3868
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3705..3724
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3916..3925
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3922..3924,3925..3927,3928..4638)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3922..3924,3925..3927,3928..4638)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3967..3988)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4228..4247)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4575..4596
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5234..5822
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5287..5307)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5705..5716)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5825..5841)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5826..5842)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6347..6527
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6553..6570)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6559..6783
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6829..7257
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6916..6935)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7126..7147
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7266..7286)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7271..7600
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7451..7586
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7513..7532
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7648..7695
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7714..8088
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8218..8339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8255..8274)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8309..8328
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8388..8404)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8388..8404)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8401..8423)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8412..8428
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8436..8442,8443..8460,8461..8466))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8481..8502
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8619..8636)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8790..9378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8870..8889)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9549..10340,10341..10409))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10172..10191
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10410..10514)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
       61 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
      121 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
      181 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
      241 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      301 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      361 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      421 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      481 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      541 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      601 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      661 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      721 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      781 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta
      841 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
      901 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
      961 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     1021 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     1081 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     1141 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     1201 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     1261 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     1321 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     1381 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     1441 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     1501 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     1561 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     1621 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     1681 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     1741 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     1801 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     1861 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     1921 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     1981 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     2041 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     2101 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     2161 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     2221 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     2281 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     2341 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     2401 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     2461 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     2521 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     2581 gacagcagag atccagtttg gttaatcgca tgctagctag ttcgaacctt aattaacccg
     2641 tgtcggctcc agatctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
     2701 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
     2761 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
     2821 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
     2881 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
     2941 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
     3001 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
     3061 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
     3121 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
     3181 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
     3241 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
     3301 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
     3361 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
     3421 ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc
     3481 gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca
     3541 cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg
     3601 gtaggctctc ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg
     3661 tcagtttctt tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg
     3721 aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat
     3781 gtaatcattt gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt
     3841 ctggccgttt ttggcttttt tgttagacga agcttgggct gcaggtcgac tctagaggat
     3901 ccccgggtac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     3961 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     4021 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     4081 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     4141 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4201 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4261 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4321 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     4381 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     4441 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4501 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     4561 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4621 atggacgagc tgtacaagtc cggactcaga tctcgagcta tgaatgggtt cagcaccgag
     4681 gaggacagcc gggatgggcc gcccgccgcc cccttttacg gccagagctg ctgcctcatc
     4741 gacgacgggg accgctgcgt gcgccccgcc ggcaacgcgt ccttcagcaa gaggatccag
     4801 aagagcatct cgcagaagaa gctgaagctg gacatcgaca aaagtgtgag gcatctgtat
     4861 atctgtgatt ttcacaagaa cttcattcaa agtgttcgga acaaaaggaa gaggaagacg
     4921 agcgatgatg gaggtgactc tcctgagcac gacacggatg ttccagaggt tgacttgttc
     4981 cagctccaag tgaacaccct gcgacgttac aagcgacatt ataaattgca gactaggcct
     5041 ggactcaaca aggctcagct tgcagaaact gtcagtcgtc atttcaggaa tattccagtg
     5101 aatgagaagg agacgcttgc atatttcatc tacatggtga agaacaataa gagcaggctg
     5161 gaccagaaat cagaaggcag caaacaactt gactgaagga ttaacataag aattcgatat
     5221 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     5281 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     5341 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     5401 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     5461 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     5521 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     5581 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     5641 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     5701 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5761 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5821 gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     5881 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     5941 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     6001 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     6061 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     6121 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     6181 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     6241 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     6301 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     6361 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6421 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6481 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
     6541 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     6601 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     6661 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     6721 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     6781 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     6841 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6901 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6961 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7021 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     7081 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7141 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7201 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     7261 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     7321 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     7381 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     7441 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7501 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     7561 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     7621 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     7681 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
     7741 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     7801 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     7861 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     7921 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     7981 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     8041 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     8101 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     8161 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     8221 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8281 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8341 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     8401 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8461 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8521 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8581 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8641 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8701 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8761 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8821 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8881 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8941 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     9001 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     9061 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     9121 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     9181 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     9241 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     9301 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     9361 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     9421 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     9481 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9541 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9601 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9661 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9721 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9781 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9841 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9901 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9961 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
    10021 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
    10081 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
    10141 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
    10201 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
    10261 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
    10321 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
    10381 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
    10441 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
    10501 ataggggttc cgcgcacatt tccccgaaaa gtgc
//
