LOCUS       sequence-32973-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J.
  TITLE     Dual RMCE for efficient re-engineering of mouse mutant alleles
  JOURNAL   Nat Methods. 2010 Oct 17
  PUBMED    20953177
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Oct 17"
COMMENT     Sequence Label: pDRAV-3
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        7..25
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(49..82)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(89..122)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 gctcgaaatt aaccctcact aaagggaaca aaagctggta ccaagcttga agttcctata
       61 ctttctagag aataggaact tcatcgatat aacttcgtat aggatacttt atacgaagtt
      121 atgtcgacga ccctacgccc ccaactgaga gaactcaaag gttaccccag ttggggcaca
      181 cgcgtaacaa tactgagaat gaagtgtatg tggaacagag gctgctgatc tcgttcttca
      241 ggctatgaaa ctgacacatt tggaaaccac agtacttaga accacaaagt gggaatcaag
      301 agaaaaacaa tgatcccacg agagatctat agatcatgag tgggaggaat gagctggccc
      361 ttaatttggt tttgcttgtt taaattatga tatccaacta tgaaacatta tcataaagca
      421 atagtaaaga gccttcagta aagagcaggc atttatctaa tcccacccca cccccacccc
      481 cgtagctcca atccttccat tcaaaatgta ggtactctgt tctcaccctt cttaacaaag
      541 tatgacagga aaaacttcca ttttagtgga catctttatt gtttaataga tcatcaattt
      601 ctcgactatt cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt
      661 acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg
      721 acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca
      781 tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gacc
//
