LOCUS       sequence-329731-       10058 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fusion of CRY2PHR with mCherry and YAP-C-IDR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Yu M, Peng Z, Qin M, Liu Y, Wang J, Zhang C, Lin J, Dong T, Wang L, 
            Li S, Yang Y, Xu S, Guo W, Zhang X, Shi M, Peng H, Luo X, Zhang H, 
            Zhang L, Li Y, Yang XP, Sun S
  TITLE     Interferon-gamma induces tumor resistance to anti-PD-1 immunotherapy
            by promoting YAP phase separation.
  JOURNAL   Mol Cell. 2021 Feb 13. pii: S1097-2765(21)00010-1. doi: 
            10.1016/j.molcel.2021.01.010.
  PUBMED    33606996
REFERENCE   2  (bases 1 to 10058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Feb 13. pii: S1097-2765(21)00010-1. doi: 
            10.1016/j.molcel.2021.01.010."
COMMENT     Sequence Label: Lenti-Cry2-mCherry- YAP-C-IDR
FEATURES             Location/Qualifiers
     source          1..10058
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3423..3432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3435..3455
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4851..6344
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    6448..7036
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6501..6521)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6919..6930)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7111..7344
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7463..7481)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7549..7653
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7654..7722,7723..8514)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7872..7891)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8685..9273
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9174..9193
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9427..9444
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9684..9705
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9720..9725,9726..9743,9744..9750)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9758..9774
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9763..9785
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9782..9798
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9782..9798
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9848..9867
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc accggtctgc
     3421 aggccaccat ggaccccaag aagaagagga aggtgcgttt tgccatgaac cagagaatca
     3481 gtcagagtgc tccagtgaaa cagccaccac ccctggctcc ccagagccca cagggaggcg
     3541 tcatgggtgg cagcaactcc aaccagcagc aacagatgcg actgcagcaa ctgcagatgg
     3601 agaaggagag gctgcggctg aaacagcaag aactgcttcg gcaggcaatg cggaatatca
     3661 atcccagcac agcaaattct ccaaaatgtc aggagttagc cctgcgtagc cagttaccaa
     3721 cactggagca ggatggtggg actcaaaatc cagtgtcttc tcccgggatg tctcaggaat
     3781 tgagaacaat gacgaccaat agctcagatc ctttccttaa cagtggcacc tatcactctc
     3841 gagatgagag tacagacagt ggactaagca tgagcagcta cagtgtccct cgaaccccag
     3901 atgacttcct gaacagtgtg gatgagatgg atacaggtga tactatcaac caaagcaccc
     3961 tgccctcaca gcagaaccgt ttcccagact accttgaagc cattcctggg acaaatgtgg
     4021 accttggaac actggaagga gatggaatga acatagaagg agaggagctg atgccaagtc
     4081 tgcaggaagc tttgagttct gacatcctta atctcgagtc tagaggatcc atggtgtcta
     4141 aaggcgagga ggataacatg gctataatca aggaatttat gaggttcaag gtgcatatgg
     4201 agggatctgt gaacggtcac gagttcgaaa tcgagggcga aggcgagggg cgaccctatg
     4261 aagggacaca gacggccaaa ctgaaggtga ccaagggtgg ccccctgcct ttcgcctggg
     4321 acatcctgtc accccagttt atgtacggct ctaaggctta cgttaagcac cctgccgata
     4381 ttccggacta cttgaaactg agcttccctg aagggttcaa gtgggaacgg gtaatgaatt
     4441 ttgaggatgg cggtgtagtc acagttaccc aggacagctc tcttcaggac ggagaattta
     4501 tctataaggt taaacttcgg ggcactaact tcccatcaga cggccccgtg atgcagaaga
     4561 aaactatggg ctgggaagcc agctcagaac gcatgtatcc cgaggacgga gcactgaagg
     4621 gagaaatcaa gcagcgactc aagctgaagg atgggggaca ttatgatgct gaggtcaaaa
     4681 ccacctataa ggccaagaaa cccgttcagc ttcctggggc atacaacgtg aatatcaaac
     4741 tggatattac atcccataac gaagactata ccatcgtgga acagtacgag cgggccgagg
     4801 gcagacatag cacagggggc atggatgaat tgtacaaggc ggccacgcgt atgaagatgg
     4861 acaaaaagac tatagtttgg tttagaagag acctaaggat tgaggataat cctgcattag
     4921 cagcagctgc tcacgaagga tctgtttttc ctgtcttcat ttggtgtcct gaagaagaag
     4981 gacagtttta tcctggaaga gcttcaagat ggtggatgaa acaatcactt gctcacttat
     5041 ctcaatcctt gaaggctctt ggatctgacc tcactttaat caaaacccac aacacgattt
     5101 cagcgatctt ggattgtatc cgcgttaccg gtgctacaaa agtcgtcttt aaccacctct
     5161 atgatcctgt ttcgttagtt cgggaccata ccgtaaagga gaagctggtg gaacgtggga
     5221 tctctgtgca aagctacaat ggagatctat tgtatgaacc gtgggagata tactgcgaaa
     5281 agggcaaacc ttttacgagt ttcaattctt actggaagaa atgcttagat atgtcgattg
     5341 aatccgttat gcttcctcct ccttggcggt tgatgccaat aactgcagcg gctgaagcga
     5401 tttgggcgtg ttcgattgaa gaactagggc tggagaatga ggccgagaaa ccgagcaatg
     5461 cgttgttaac tagagcttgg tctccaggat ggagcaatgc tgataagtta ctaaatgagt
     5521 tcatcgagaa gcagttgata gattatgcaa agaacagcaa gaaagttgtt gggaattcta
     5581 cttcactact ttctccgtat ctccatttcg gggaaataag cgtcagacac gttttccagt
     5641 gtgcccggat gaaacaaatt atatgggcaa gagataagaa cagtgaagga gaagaaagtg
     5701 cagatctttt tcttagggga atcggtttaa gagagtattc tcggtatata tgtttcaact
     5761 tcccgtttac tcacgagcaa tcgttgttga gtcatcttcg gtttttccct tgggatgctg
     5821 atgttgataa gttcaaggcc tggagacaag gcaggaccgg ttatccgttg gtggatgccg
     5881 gaatgagaga gctttgggct accggatgga tgcataacag aataagagtg attgtttcaa
     5941 gctttgctgt gaagtttctt ctccttccat ggaaatgggg aatgaagtat ttctgggata
     6001 cacttttgga tgctgatttg gaatgtgaca tccttggctg gcagtatatc tctgggagta
     6061 tccccgatgg ccacgagctt gatcgcttgg acaatcccgc gttacaaggc gccaaatatg
     6121 acccagaagg tgagtacata aggcaatggc ttcccgagct tgcgagattg ccaactgaat
     6181 ggatccatca tccatgggac gctcctttaa ccgtactcaa agcttctggt gtggaactcg
     6241 gaacaaacta tgcgaaaccc attgtagaca tcgacacagc tcgtgagcta ctagctaaag
     6301 ctatttcaag aacccgtgaa gcacagatca tgatcggagc agcagcccgg gatccaccgg
     6361 tcgccaccgg ttcagggagc ggatccggct cagggagtgc ggccgcaact cccacctgca
     6421 acatgcgtga ctgaatcgat agatcctaat caacctctgg attacaaaat ttgtgaaaga
     6481 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6541 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6601 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6661 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6721 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6781 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6841 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6901 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     6961 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     7021 tgggccgcct ccccgcctga gatcctttaa gaccaatgac ttacaaggca gctgtagatc
     7081 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7141 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7201 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7261 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7321 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     7381 aacttgcaaa gaaatgaata tcagagagtg agaggcccgg gttaattaag gaaagggcta
     7441 gatcattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     7501 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     7561 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7621 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7681 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7741 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7801 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7861 tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     7921 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7981 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8041 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8101 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8161 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     8221 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8281 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8341 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8401 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8461 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8521 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8581 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8641 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8701 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8761 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8821 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8881 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8941 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9001 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9061 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9121 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9181 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9241 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9301 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     9361 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     9421 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     9481 cccgcgcgtt ggccgattca ttaatgcagc aagctcatgg ctgactaatt ttttttattt
     9541 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     9601 ttggaggcct aggcttttgc aaaaagctcc ccgtggcacg acaggtttcc cgactggaaa
     9661 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     9721 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     9781 acaggaaaca gctatgacat gattacgaat ttcacaaata aagcattttt ttcactgcat
     9841 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata
     9901 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat
     9961 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga
    10021 aattgtattt gttaaatatg tactacaaac ttagtagt
//
