LOCUS       sequence-329733-        9902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fusion of CRY2PHR with mCherry and YAP-N-IDR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9902)
  AUTHORS   Yu M, Peng Z, Qin M, Liu Y, Wang J, Zhang C, Lin J, Dong T, Wang L, 
            Li S, Yang Y, Xu S, Guo W, Zhang X, Shi M, Peng H, Luo X, Zhang H, 
            Zhang L, Li Y, Yang XP, Sun S
  TITLE     Interferon-gamma induces tumor resistance to anti-PD-1 immunotherapy
            by promoting YAP phase separation.
  JOURNAL   Mol Cell. 2021 Feb 13. pii: S1097-2765(21)00010-1. doi: 
            10.1016/j.molcel.2021.01.010.
  PUBMED    33606996
REFERENCE   2  (bases 1 to 9902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Feb 13. pii: S1097-2765(21)00010-1. doi: 
            10.1016/j.molcel.2021.01.010."
COMMENT     Sequence Label: Lenti-Cry2-mCherry- YAP-N-IDR
FEATURES             Location/Qualifiers
     source          1..9902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3423..3432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3435..3455
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4695..6188
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    6292..6880
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6345..6365)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6763..6774)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6955..7188
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7307..7325)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7393..7497
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7498..7566,7567..8358)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7716..7735)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8529..9117
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9018..9037
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9271..9288
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9528..9549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9564..9569,9570..9587,9588..9594)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9602..9618
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9607..9629
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9626..9642
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9626..9642
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9692..9711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc accggtctgc
     3421 aggccaccat ggaccccaag aagaagagga aggtgatgga ccccgggcag cagccgccgc
     3481 ctcaaccggc cccccagggc caagggcagc cgccttcgca gcccccgcag gggcagggcc
     3541 cgccgtccgg acccgggcaa ccggcacccg cggcgaccca ggcggcgccg caggcacccc
     3601 ccgccgggca tcagatcgtg cacgtccgcg gggactcgga gaccgacctg gaggcgctct
     3661 tcaacgccgt catgaacccc aagacggcca acgtgcccca gaccgtgccc atgaggctcc
     3721 ggaagctgcc cgactccttc ttcaagccgc cggagcccaa atcccactcc cgacaggcca
     3781 gtactgatgc aggcactgca ggagccctga ctccacagca tgttcgagct cattcctctc
     3841 cagcttctct gcagttggga gctgtttctc ctgggacact gacccccact ggagtagtct
     3901 ctggcccagc agctacaccc acagctcagc atcttcgaca gtcttctttt gagatactcg
     3961 agtctagagg atccatggtg tctaaaggcg aggaggataa catggctata atcaaggaat
     4021 ttatgaggtt caaggtgcat atggagggat ctgtgaacgg tcacgagttc gaaatcgagg
     4081 gcgaaggcga ggggcgaccc tatgaaggga cacagacggc caaactgaag gtgaccaagg
     4141 gtggccccct gcctttcgcc tgggacatcc tgtcacccca gtttatgtac ggctctaagg
     4201 cttacgttaa gcaccctgcc gatattccgg actacttgaa actgagcttc cctgaagggt
     4261 tcaagtggga acgggtaatg aattttgagg atggcggtgt agtcacagtt acccaggaca
     4321 gctctcttca ggacggagaa tttatctata aggttaaact tcggggcact aacttcccat
     4381 cagacggccc cgtgatgcag aagaaaacta tgggctggga agccagctca gaacgcatgt
     4441 atcccgagga cggagcactg aagggagaaa tcaagcagcg actcaagctg aaggatgggg
     4501 gacattatga tgctgaggtc aaaaccacct ataaggccaa gaaacccgtt cagcttcctg
     4561 gggcatacaa cgtgaatatc aaactggata ttacatccca taacgaagac tataccatcg
     4621 tggaacagta cgagcgggcc gagggcagac atagcacagg gggcatggat gaattgtaca
     4681 aggcggccac gcgtatgaag atggacaaaa agactatagt ttggtttaga agagacctaa
     4741 ggattgagga taatcctgca ttagcagcag ctgctcacga aggatctgtt tttcctgtct
     4801 tcatttggtg tcctgaagaa gaaggacagt tttatcctgg aagagcttca agatggtgga
     4861 tgaaacaatc acttgctcac ttatctcaat ccttgaaggc tcttggatct gacctcactt
     4921 taatcaaaac ccacaacacg atttcagcga tcttggattg tatccgcgtt accggtgcta
     4981 caaaagtcgt ctttaaccac ctctatgatc ctgtttcgtt agttcgggac cataccgtaa
     5041 aggagaagct ggtggaacgt gggatctctg tgcaaagcta caatggagat ctattgtatg
     5101 aaccgtggga gatatactgc gaaaagggca aaccttttac gagtttcaat tcttactgga
     5161 agaaatgctt agatatgtcg attgaatccg ttatgcttcc tcctccttgg cggttgatgc
     5221 caataactgc agcggctgaa gcgatttggg cgtgttcgat tgaagaacta gggctggaga
     5281 atgaggccga gaaaccgagc aatgcgttgt taactagagc ttggtctcca ggatggagca
     5341 atgctgataa gttactaaat gagttcatcg agaagcagtt gatagattat gcaaagaaca
     5401 gcaagaaagt tgttgggaat tctacttcac tactttctcc gtatctccat ttcggggaaa
     5461 taagcgtcag acacgttttc cagtgtgccc ggatgaaaca aattatatgg gcaagagata
     5521 agaacagtga aggagaagaa agtgcagatc tttttcttag gggaatcggt ttaagagagt
     5581 attctcggta tatatgtttc aacttcccgt ttactcacga gcaatcgttg ttgagtcatc
     5641 ttcggttttt cccttgggat gctgatgttg ataagttcaa ggcctggaga caaggcagga
     5701 ccggttatcc gttggtggat gccggaatga gagagctttg ggctaccgga tggatgcata
     5761 acagaataag agtgattgtt tcaagctttg ctgtgaagtt tcttctcctt ccatggaaat
     5821 ggggaatgaa gtatttctgg gatacacttt tggatgctga tttggaatgt gacatccttg
     5881 gctggcagta tatctctggg agtatccccg atggccacga gcttgatcgc ttggacaatc
     5941 ccgcgttaca aggcgccaaa tatgacccag aaggtgagta cataaggcaa tggcttcccg
     6001 agcttgcgag attgccaact gaatggatcc atcatccatg ggacgctcct ttaaccgtac
     6061 tcaaagcttc tggtgtggaa ctcggaacaa actatgcgaa acccattgta gacatcgaca
     6121 cagctcgtga gctactagct aaagctattt caagaacccg tgaagcacag atcatgatcg
     6181 gagcagcagc ccgggatcca ccggtcgcca ccggttcagg gagcggatcc ggctcaggga
     6241 gtgcggccgc aactcccacc tgcaacatgc gtgactgaat cgatagatcc taatcaacct
     6301 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6361 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6421 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6481 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6541 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6601 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     6661 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     6721 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     6781 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     6841 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa
     6901 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6961 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     7021 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7081 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7141 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     7201 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     7261 ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc
     7321 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     7381 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7441 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     7501 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     7561 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     7621 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     7681 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     7741 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     7801 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     7861 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     7921 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     7981 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     8041 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     8101 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     8161 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     8221 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     8281 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     8341 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     8401 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     8461 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     8521 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     8581 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     8641 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     8701 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     8761 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     8821 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     8881 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     8941 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     9001 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     9061 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     9121 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     9181 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     9241 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     9301 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc
     9361 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     9421 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg
     9481 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     9541 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     9601 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca
     9661 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9721 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     9781 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     9841 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     9901 gt
//
