LOCUS       sequence-32975-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J.
  TITLE     Dual RMCE for efficient re-engineering of mouse mutant alleles
  JOURNAL   Nat Methods. 2010 Oct 17
  PUBMED    20953177
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Oct 17"
COMMENT     Sequence Label: pDRAV-4
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..71
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(78..111)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 accctcacta aagggaacaa aagctggtac caagcttgaa gttcctattc tctagaaagt
       61 ataggaactt catcgatata acttcgtata ggatacttta tacgaagtta tgtcgacgac
      121 cctacgcccc caactgagag aactcaaagg ttaccccagt tggggcacac gcgtaacaat
      181 actgagaatg aagtgtatgt ggaacagagg ctgctgatct cgttcttcag gctatgaaac
      241 tgacacattt ggaaaccaca gtacttagaa ccacaaagtg ggaatcaaga gaaaaacaat
      301 gatcccacga gagatctata gatcatgagt gggaggaatg agctggccct taatttggtt
      361 ttgcttgttt aaattatgat atccaactat gaaacattat cataaagcaa tagtaaagag
      421 ccttcagtaa agagcaggca tttatctaat cccaccccac ccccaccccc gtagctccaa
      481 tccttccatt caaaatgtag gtactctgtt ctcacccttc ttaacaaagt atgacaggaa
      541 aaacttccat tttagtggac atctttattg tttaatagat catcaatttc tcgactattc
      601 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
      661 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
      721 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
      781 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg
      841 c
//
