LOCUS       sequence-329759-        7983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fusion of mEGFP and LATS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Yu M, Peng Z, Qin M, Liu Y, Wang J, Zhang C, Lin J, Dong T, Wang L, 
            Li S, Yang Y, Xu S, Guo W, Zhang X, Shi M, Peng H, Luo X, Zhang H, 
            Zhang L, Li Y, Yang XP, Sun S
  TITLE     Interferon-gamma induces tumor resistance to anti-PD-1 immunotherapy
            by promoting YAP phase separation.
  JOURNAL   Mol Cell. 2021 Feb 13. pii: S1097-2765(21)00010-1. doi: 
            10.1016/j.molcel.2021.01.010.
  PUBMED    33606996
REFERENCE   2  (bases 1 to 7983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Feb 13. pii: S1097-2765(21)00010-1. doi: 
            10.1016/j.molcel.2021.01.010."
COMMENT     Sequence Label: mEGFP-N1-LATS2
FEATURES             Location/Qualifiers
     source          1..7983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      3..12
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9..11,12..14,15..728)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9..11,12..14,15..725)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(54..75)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(315..334)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     662..683
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    851..972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(888..907)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     942..961
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(979..1434)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1116..1137)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1328..1347
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1461..1565
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1567..1924
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1567..1758
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1590..1610)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      1775..1910
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1959..2753
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1959..2753
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2013..2032)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2623..2642
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2941..2960)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2985..3032
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3361..3949
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3850..3869
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4124..4427
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4428..4631
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4582..4602
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
       61 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      121 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      181 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
      241 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      301 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      361 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      421 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
      481 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      541 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      601 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      661 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      721 acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
      781 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
      841 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      901 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
      961 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     1021 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     1081 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     1141 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     1201 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     1261 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     1321 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     1381 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     1441 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1501 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     1561 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     1621 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     1681 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     1741 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     1801 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1861 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1921 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     1981 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     2041 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     2101 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     2161 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     2221 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     2281 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     2341 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     2401 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     2461 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     2521 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     2581 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     2641 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     2701 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     2761 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     2821 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     2881 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     2941 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     3001 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     3061 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     3121 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     3181 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     3241 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3301 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3361 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3421 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3481 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3541 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3601 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3661 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3721 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3781 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3841 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3901 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3961 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4021 gttatcccct gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat
     4081 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     4141 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     4201 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     4261 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     4321 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     4381 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     4441 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     4501 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     4561 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     4621 taagcagagc tggtttagtg aaccgtcaga tccgctagcg ctaccggact cagatctcga
     4681 gctcaagctt cgaattcatg aggccaaaga cttttcctgc cacgacttat tctggaaata
     4741 gccggcagcg actgcaagag attcgtgagg ggttaaagca gccatccaag tcttcggttc
     4801 aggggctacc cgcaggacca aacagtgaca cttccctgga tgccaaagtc ctggggagca
     4861 aagatgccac caggcagcag cagcagatga gagccacccc aaagttcgga ccttatcaga
     4921 aagccttgag ggaaatcaga tattccttgt tgccttttgc taatgaatcg ggcacctctg
     4981 cagctgcaga agtgaaccgg caaatgctgc aggaactggt gaacgcagga tgcgaccagg
     5041 agatggctgg ccgagctctc aagcagactg gcagcaggag catcgaggcc gccctggagt
     5101 acatcagcaa gatgggctac ctggacccga ggaatgagca gattgtgcgg gtcattaagc
     5161 agacctcccc aggaaagggg ctcatgccaa ccccagtgac gcggaggccc agcttcgaag
     5221 gaaccggcga ttcgtttgcg tcctaccacc agctgagcgg taccccctac gagggcccaa
     5281 gcttcggcgc tgacggcccc acggcgctgg aggagatgcc gcggccgtac gtggactacc
     5341 ttttccccgg agtcggcccc cacgggcccg gccaccagca ccagcaccca cccaagggct
     5401 acggtgccag cgtagaggca gcaggggcac acttcccgct gcagggcgcg cactacgggc
     5461 ggccgcacct gctggtgcct ggggaacccc tgggctacgg agtgcagcgc agcccctcct
     5521 tccagagcaa gacgccgccg gagaccgggg gttacgccag cctgcccacg aagggccagg
     5581 gaggaccgcc aggcgccggc ctcgctttcc caccccctgc cgccgggctc tacgtgccgc
     5641 acccacacca caagcaggcc ggtcccgcgg cccaccagct gcatgtgctg ggctcccgca
     5701 gccaggtgtt cgccagcgac agccccccgc agagcctgct cactccctcg cggaacagcc
     5761 tcaacgtgga cctgtatgaa ttgggcagca cctccgtcca gcagtggccg gctgccaccc
     5821 tggcccgccg ggactccctg cagaagccgg gcctggaggc gccgccgcgc gcgcacgtgg
     5881 ccttccggcc tgactgccca gtgcccagca ggaccaactc cttcaacagc caccagccgc
     5941 ggcccggtcc gcctggcaag gccgagccct ccctgcccgc ccccaacacc gtgacggctg
     6001 tcacggccgc gcacatcttg cacccggtga agagcgtgcg tgtgctgagg ccggagccgc
     6061 agacggctgt ggggccctcg caccccgcct gggtgcccgc gcctgccccg gcccccgccc
     6121 ccgcccccgc cccggctgcg gagggcttgg acgccaagga ggagcatgcc ctggcgctgg
     6181 gcggcgcagg cgccttcccg ctggacgtgg agtacggagg cccagaccgg aggtgcccgc
     6241 ctccgcccta cccgaagcac ctgctgctgc gcagcaagtc ggagcagtac gacctggaca
     6301 gcctgtgcgc aggcatggag cagagcctcc gtgcgggccc caacgagccc gagggcggcg
     6361 acaagagccg caaaagcgcc aagggggaca aaggcggaaa ggataaaaag cagattcaga
     6421 cctctcccgt tcccgtccgc aaaaacagca gagacgaaga gaagagagag tcacgcatca
     6481 agagctactc gccatacgcc tttaagttct tcatggagca gcacgtggag aatgtcatca
     6541 aaacctacca gcagaaggtt aaccggaggc tgcagctgga gcaagaaatg gccaaagctg
     6601 gactctgtga agctgagcag gagcagatgc ggaagatcct ctaccagaaa gagtctaatt
     6661 acaacaggtt aaagagggcc aagatggaca agtctatgtt tgtcaagatc aaaaccctgg
     6721 ggatcggtgc ctttggagaa gtgtgccttg cttgtaaggt ggacactcac gccctgtacg
     6781 ccatgaagac cctaaggaaa aaggatgtcc tgaaccggaa tcaggtggcc cacgtcaagg
     6841 ccgagaggga catcctggcc gaggcagaca atgagtgggt ggtcaaactc tactactcct
     6901 tccaagacaa agacagcctg tactttgtga tggactacat ccctggtggg gacatgatga
     6961 gcctgctgat ccggatggag gtcttccctg agcacctggc ccggttctac atcgcagagc
     7021 tgactttggc cattgagagt gtccacaaga tgggcttcat ccaccgagac atcaagcctg
     7081 ataacatttt gatagatctg gatggtcaca ttaaactcac agatttcggc ctctgcactg
     7141 ggttcaggtg gactcacaat tccaaatatt accagaaagg gagccatgtc agacaggaca
     7201 gcatggagcc cagcgacctc tgggatgatg tgtctaactg tcggtgtggg gacaggctga
     7261 agaccctaga gcagagggcg cggaagcagc accagaggtg cctggcacat tcactggtgg
     7321 ggactccaaa ctacatcgca cccgaggtgc tcctccgcaa agggtacact caactctgtg
     7381 actggtggag tgttggagtg attctcttcg agatgctggt ggggcagccg ccctttttgg
     7441 cacctactcc cacagaaacc cagctgaagg tgatcaactg ggagaacacg ctccacattc
     7501 cagcccaggt gaagctgagc cctgaggcca gggacctcat caccaagctg tgctgctccg
     7561 cagaccaccg cctggggcgg aatggggccg atgacctgaa ggcccacccc ttcttcagcg
     7621 ccattgactt ctccagtgac atccggaagc agccagcccc ctacgttccc accatcagcc
     7681 accccatgga cacctcgaat ttcgaccccg tagatgaaga aagcccttgg aacgatgcca
     7741 gcgaaggtag caccaaggcc tgggacacac tcacctcgcc caataacaag catcctgagc
     7801 acgcatttta cgaattcacc ttccgaaggt tctttgatga caatggctac ccctttcgat
     7861 gcccaaagcc ttcaggagca gaagcttcac aggctgagag ctcagattta gaaagctctg
     7921 atctggtgga tcagactgaa ggctgccagc ctgtgtacgt gggtggaggc gggtctccac
     7981 cgg
//
