LOCUS       sequence-32976-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J.
  TITLE     Dual RMCE for efficient re-engineering of mouse mutant alleles
  JOURNAL   Nat Methods. 2010 Oct 17
  PUBMED    20953177
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Oct 17"
COMMENT     Sequence Label: pDRAV-4
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(4..37)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    98..131
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        159..658
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(194..217)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     591..610
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ctcataactt cgtatagcat acattatacg aagttatggc gcctcagcgg ccgcacaatg
       61 catgtagtta acttcttaat taactgggat cctgcgggtg ccagggcgtg cccttgggct
      121 ccccgggcgc gtactccacc tcacctcgag attctaccgg gtaggggagg cgcttttccc
      181 aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg
      241 cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg
      301 tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca
      361 gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat
      421 ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct
      481 ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag
      541 gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac
      601 gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac
      661 cgatccagcc gccaccatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
      721 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcgganggcg aagaatctcg
      781 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
      841 tggtttctac aaagatcgtt atgtttat
//
