LOCUS       sequence-329762-         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CHD7 in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Bouazoune K, Kingston RE
  TITLE     Chromatin remodeling by the CHD7 protein is impaired by mutations 
            that cause human developmental disorders.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 Nov 20;109(47):19238-43. doi: 
            10.1073/pnas.1213825109. Epub 2012 Nov 7.
  PUBMED    23134727
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1213825109"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2012-11-20- 20"; volume: "109"; issue: "47"; pages: 
            "19238-43"
COMMENT     Sequence Label: pFastBac-CHD7
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             69..89
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     regulatory      431..440
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcgcgcggaa ttcaaaggcc tacgtcgaca agatgcatca tcatcatcat cacggtggtg
       61 gtggtggtga aaacctgtac ttccagggaa tggcagatcc aggaatgatg agtctttttg
      121 gcgaggatgg gaatattttc agtgaaggtc ttgaaggcct cggagaatgt ggttacccgg
      181 aaaatccagt aaatcctatg ggtcagcaaa tgccaataga ccaaggcttt gcctctttac
      241 agccatccct tcatcatcct tcaactaatc aaaatcaaac aaagctgaca cattttgatc
      301 actataatca gtatgaacaa caaaagatgc atctgatgga tcagccgaac agaatgatga
      361 gcaacacccc tgggaacgga ctcgcgtctc cgcactcgca gtatcacacc cctcccgttc
      421 ctcaggtgcc ccatggtggc agtggtggcg gtcagatggg tgtctaccct ggcatgcaga
      481 atgagaggca tgggcaatcc tttgtggaca gcagctccat gtggggcccc agggctgttc
      541 aggtaccaga ccagatacga gccccctacc agcagcagca gccacagccg cagccaccgc
      601 agccggctcc gtcggggccc cctgcacagg gccaccctca gcacatgcag cagatgggca
      661 gctatatggc acgtggggat ttttccatgc agcagcatgg tcagccacag cag
//
