LOCUS       sequence-32977-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J.
  TITLE     Dual RMCE for efficient re-engineering of mouse mutant alleles
  JOURNAL   Nat Methods. 2010 Oct 17
  PUBMED    20953177
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Oct 17"
COMMENT     Sequence Label: pDREV-0
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             82..135
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             142..516
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAVTKYTSAK"
     primer_bind     complement(568..589)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aaccagtaac ctctgccctt tctcctccat gacaaccagg tcccaggtcc cgaaaaccaa
       61 agaagaagaa cgcagatctc cgagggcaga ggaagtcttc taacatgcgg tgacgtggag
      121 gagaatcccg gaccgggggg accagagcca gcgaagtctg ctcccgcccc gaaaaagggc
      181 tccaagaagg cggtgactaa ggcgcagaag aaagacggca agaagcgcaa gcgcagccgc
      241 aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc
      301 atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc
      361 gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag
      421 atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag
      481 ggtactaagg ccgtcaccaa gtacaccagc gctaaatcca ccatggtgag caagggcgag
      541 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      601 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      661 ctgatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgggc
      721 tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
      781 tccgccatgc ccgaaggcta cgtccaggag cgca
//
