LOCUS       sequence-329775-       11228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FAP (dL5**) fused to mCerulean and the telomere binding 
            protein TRF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Fouquerel E, Barnes RP, Uttam S, Watkins SC, Bruchez MP, Opresko PL
  TITLE     Targeted and Persistent 8-Oxoguanine Base Damage at Telomeres 
            Promotes Telomere Loss and Crisis.
  JOURNAL   Mol Cell. 2019 Jul 11;75(1):117-130.e6. doi: 
            10.1016/j.molcel.2019.04.024. Epub 2019 May 14.
  PUBMED    31101499
REFERENCE   2  (bases 1 to 11228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.04.024"; journalName: "Mol Cell"; date: 
            "2019-07-11- 11"; volume: "75"; issue: "1"; pages: "117-130.e6"
COMMENT     Sequence Label: pLVX-dL5**-mCER-TRF1
FEATURES             Location/Qualifiers
     source          1..11228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2823..2847
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2871..3590
                     /codon_start=1
                     /product="fluorogen-activating protein composed of a dimer 
                     of the L5-MG single-chain antibody fragment (Szent-Gyorgyi 
                     et al., 2008)"
                     /label=FAP-alpha-2
                     /note="binds malachite green derivatives to produce red 
                     fluorescence"
                     /translation="QAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGHYANWFQQKPGQA
                     PRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSLSDVDGYLFGGGT
                     QLTVLSGGGGSGGGGSGGGGSGGGGSQAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGH
                     YANWFQQKPGQAPRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSL
                     SDVDGYLFGGGTQLTVLS"
     CDS             join(3648..3650,3651..3653,3654..4364)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3693..3714)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3954..3973)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4301..4322
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(5746..5770)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5802..6376,6377..6379,6380..6388)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5969..5986)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6196..6215
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6389..7183
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6443..6462)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7053..7072
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    7197..7785
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7250..7270)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7668..7679)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7992..8625
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8753..8769)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8753..8769)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8766..8788)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8777..8793
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8801..8807,8808..8825,8826..8831))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8846..8867
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8984..9001)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9155..9743)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9235..9254)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9914..10705,10706..10774))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10537..10556
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10775..10879)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10927..11061
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10964..10983)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11018..11037
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactaat
     2821 caacaagttt gtacaaaaaa gcaggctccg cggccgcccc cttcaccatg caggccgtcg
     2881 ttacccaaga acctagtgtt accgttagcc caggtggtac tgttatactt acttgtggaa
     2941 gtggtacggg tgccgtcaca tctggtcatt atgcaaattg gtttcaacaa aaaccaggac
     3001 aagctccaag agctttgatt tttgatactg ataagaagta ttcttggacc ccaggtagat
     3061 tttctggatc tttgctggga gcaaaggcag ctttgacaat atcagatgct cagcctgagg
     3121 acgaagccga gtattactgt tctcttagcg acgtggatgg ctacttgttt ggcggtggaa
     3181 cacaactgac ggttctgtcc ggtggtggcg gctctggtgg cggtggcagc ggcggtggtg
     3241 gttccggagg cggcggttct caggctgtgg tgactcagga gccgtcagtg actgtgtccc
     3301 caggagggac agtcattctc acttgtggct ccggcactgg agctgtcacc agtggtcatt
     3361 atgccaactg gttccagcag aagcctggcc aagcccccag ggcacttata tttgacaccg
     3421 acaagaagta ttcctggacc cctggccgat tctcaggctc cctccttggg gccaaggctg
     3481 ccctgaccat ctcggatgcg cagcctgaag atgaggctga gtattactgt tcgctctccg
     3541 acgttgacgg ttatctgttc ggaggaggca cccagctgac cgtcctctcc gcggccggtc
     3601 tggcccatat gggaggcggt gggtctggtg gcggtggcag caccggtatg gtgagcaagg
     3661 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3721 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3781 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3841 tgagctgggg cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     3901 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3961 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     4021 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     4081 acgccatcca cggcaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
     4141 acttcggcct caactgcaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     4201 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     4261 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     4321 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc
     4381 gagctcaagc ttcgaattct gcagttaaca tggcggagga tgtttcctca gcggccccga
     4441 gcccgcgggg ctgtgcggat ggtagggatg ccgaccctac tgaggagcag atggcagaaa
     4501 cagagagaaa cgacgaggag cagttcgaat gccaggaact gctcgagtgc caggtgcagg
     4561 tgggggcccc cgaggaggag gaggaggagg aggaggacgc gggcctggtg gccgaggccg
     4621 aggccgtggc tgccggctgg atgctcgatt tcctctgcct ctctctttgc cgagctttcc
     4681 gcgacggccg ctccgaggac ttccgcagga cccgcaacag cgcagaggct attattcatg
     4741 gactatccag tctaacagct tgccagttga gaacgatata catatgtcag tttttgacaa
     4801 gaattgcagc aggaaaaacc cttgatgcac agtttgaaaa tgatgaacga attacaccct
     4861 tggaatcagc cctgatgatt tggggttcaa ttgaaaagga acatgacaaa cttcatgaag
     4921 aaatacagaa tttaattaaa attcaggcta tagctgtttg tatggaaaat ggcaacttta
     4981 aagaagcaga agaagtcttt gaaagaatat ttggtgatcc aaattctcat atgcctttca
     5041 aaagcaaatt gcttatgata atctctcaga aagatacatt tcattccttt tttcaacact
     5101 tcagctacaa ccacatgatg gagaaaatta agagttatgt gaattatgtg ctaagtgaaa
     5161 aatcatcaac ctttctaatg aaggcagcgg caaaagtagt agaaagcaaa aggacaagaa
     5221 caataacttc tcaagataaa cctagtggta atgatgttga aatggaaact gaagctaatt
     5281 tggatacaag aaaaagtgtt agtgacaaac agtctgcggt aactgaatcc tcagagggta
     5341 cagtatcctt attgaggtct cacaagaatc ttttcttatc taagttgcaa catggaaccc
     5401 agcaacaaga ccttaataag aaagaaagaa gagtaggaac tcctcaaagt acaaaaaaga
     5461 aaaaagaaag cagaagagcc actgaaagca gaatacctgt ttcaaagagt cagccggtaa
     5521 ctcctgaaaa acatcgagct agaaaaagac aggcatggct ttgggaagaa gacaagaatt
     5581 tgagatctgg cgtgaggaaa tatggagagg gaaactggtc taaaatactg ttgcattata
     5641 aattcaacaa ccggacaagt gtcatgttaa aagacagatg gaggaccatg aagaaactaa
     5701 aactgatttc ctcagacagc gaagactgaa agggtgggcg cgccgaccca gctttcttgt
     5761 acaaagtggt tgatctagtt ctagagcggc cgcggatccc gcccctctcc ctcccccccc
     5821 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     5881 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     5941 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     6001 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     6061 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     6121 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     6181 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     6241 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     6301 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     6361 aaaacacgat gataagcttg ccacaaccat ggctgaacaa gatggattgc acgcaggttc
     6421 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     6481 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     6541 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     6601 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6661 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6721 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6781 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6841 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6901 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6961 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     7021 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     7081 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     7141 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaacgcgtc tggaacaatc
     7201 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     7261 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     7321 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     7381 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     7441 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     7501 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     7561 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     7621 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7681 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7741 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg
     7801 cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     7861 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     7921 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7981 aaagagggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     8041 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     8101 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     8161 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     8221 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     8281 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     8341 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     8401 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     8461 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8521 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8581 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     8641 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     8701 gacattgcta gcgtttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     8761 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8821 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8881 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8941 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     9001 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     9061 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     9121 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     9181 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     9241 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9301 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9361 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9421 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9481 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9541 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     9601 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9661 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9721 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9781 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9841 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9901 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9961 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    10021 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    10081 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    10141 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    10201 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    10261 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10321 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10381 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10441 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10501 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10561 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10621 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10681 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10741 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10801 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10861 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
    10921 gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    10981 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    11041 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
    11101 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
    11161 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
    11221 ttagtagt
//
