LOCUS       sequence-32978-e         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J.
  TITLE     Dual RMCE for efficient re-engineering of mouse mutant alleles
  JOURNAL   Nat Methods. 2010 Oct 17
  PUBMED    20953177
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Oct 17"
COMMENT     Sequence Label: pDREV-0
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..385)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVX
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAVTKYTSAK"
     CDS             complement(392..445)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    join(456..487,488..571)
                     /label=En2 SA
                     /note="splice acceptor from the mouse Engrailed 2 gene 
                     (Skarnes et al., 1992)"
ORIGIN
        1 ccatggtgga tttagcgctg gtgtacttgg tgacggcctt agtaccctcg gacacggcgt
       61 gcttggccaa ctccccaggc agcagcaggc gcacggccgt ctggatctcc ctggaggtga
      121 tggtcgagcg cttgttgtaa tgcgccaggc gggaagcctc acctgcgatg cgctcgaaaa
      181 tgtcgttcac aaacgaattc atgatgccca tggccttgga cgaaatgccg gtgtcagggt
      241 ggacctgctt canaaccttg tacacataga tggaatagct ctccttgcgg ctgcgcttgc
      301 gcttcttgcc gtctttcttc tgcgccttag tcaccgcctt cttggagccc tttttcgggg
      361 cgggagcaga cttcgctggc tctggtcccc ccggtccggg attctcctcc acgtcaccgc
      421 atgttagaag acttcctctg ccctcggaga tctgcgttct tcttctttgg ttttcgggac
      481 ctgggacctg gttgtcatgg aggagaaagg gcagaggtta ctggttgctg gagtctagct
      541 acttatccac aaccaacgca cccaagctag cttgaggttg cagatactgg gggtgggggg
      601 ggggatgacc cgccc
//
