LOCUS       sequence-32979-e         614 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J.
  TITLE     Dual RMCE for efficient re-engineering of mouse mutant alleles
  JOURNAL   Nat Methods. 2010 Oct 17
  PUBMED    20953177
REFERENCE   2  (bases 1 to 614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Oct 17"
COMMENT     Sequence Label: pDREV-1
FEATURES             Location/Qualifiers
     source          1..614
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..386)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAVTKYTSAK"
     CDS             complement(393..446)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    join(471..502,503..586)
                     /label=En2 SA
                     /note="splice acceptor from the mouse Engrailed 2 gene 
                     (Skarnes et al., 1992)"
ORIGIN
        1 accatggtgg atttagcgct ggtgtacttg gtgacggcct tagtaccctc ggacacggcg
       61 tgcttggcca actccccagg cagcagcagg cgcacggccg tctggatctc cctggaggtg
      121 atggtcgagc gcttgttgta atgcgccagg cgggaagcct cacctgcgat gcgctcgaaa
      181 atgtcgttca caaacgaatt catgatgccc atggccttgg acgaaatgcc ggtgtcaggg
      241 tggacctgct tcagaacctt gtacacatag atggaatagc tctccttgcg gctgcgcttg
      301 cgcttcttgc cgtctttctt ctgcgcctta gtcaccgcct tcttggagcc ctttttcggg
      361 gcgggagcag acttcgctgg ctctggtccc cccggtccgg gattctcctc cacgtcaccg
      421 catgttagaa gacttcctct gccctcggag atctgcgatc tgcgatctgc gttcttcttc
      481 tttggttttc gggacctggg acctggttgt catggaggag aaagggcaga ggttactggt
      541 tgctggagtc tagctactta tccacaacca acgcacccaa gctagcttga ggttgcagat
      601 actgggggtg gggg
//
