LOCUS       sequence-329798-        7822 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of Taq DNA polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   Graham TGW, Dugast-Darzacq C, Dailey GM, Nguyenla XH, Van Dis E, 
            Esbin MN, Abidi A, Stanley SA, Darzacq X, Tjian R
  TITLE     Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 
            detection.
  JOURNAL   PLoS One. 2021 Feb 3;16(2):e0246647. doi: 
            10.1371/journal.pone.0246647. eCollection 2021.
  PUBMED    33534838
REFERENCE   2  (bases 1 to 7822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2021-02-3"; volume: "16(2):e0246647. doi"; pages: " 
            10.1371/journal.pone.0246647. eCollection 2021"
COMMENT     Sequence Label: pET-28a_6H-TAQ_E602D
FEATURES             Location/Qualifiers
     source          1..7822
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..25
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             40..62
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             81..98
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             108..125
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             129..2627
                     /codon_start=1
                     /product="thermostable DNA polymerase from Thermus 
                     aquaticus"
                     /label=Taq polymerase
                     /translation="MRGMLPLFEPKGRVLLVDGHHLAYRTFHALKGLTTSRGEPVQAVY
                     GFAKSLLKALKEDGDAVIVVFDAKAPSFRHEAYGGYKAGRAPTPEDFPRQLALIKELVD
                     LLGLARLEVPGYEADDVLASLAKKAEKEGYEVRILTADKDLYQLLSDRIHVLHPEGYLI
                     TPAWLWEKYGLRPDQWADYRALTGDESDNLPGVKGIGEKTARKLLEEWGSLEALLKNLD
                     RLKPAIREKILAHMDDLKLSWDLAKVRTDLPLEVDFAKRREPDRERLRAFLERLEFGSL
                     LHEFGLLESPKALEEAPWPPPEGAFVGFVLSRKEPMWADLLALAAARGGRVHRAPEPYK
                     ALRDLKEARGLLAKDLSVLALREGLGLPPGDDPMLLAYLLDPSNTTPEGVARRYGGEWT
                     EEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSAVLAHMEATGVRLDVAYLRA
                     LSLEVAEEIARLEAEVFRLAGHPFNLNSRDQLERVLFDELGLPAIGKTEKTGKRSTSAA
                     VLEALREAHPIVEKILQYRELTKLKSTYIDPLPDLIHPRTGRLHTRFNQTATATGRLSS
                     SDPNLQNIPVRTPLGQRIRRAFIAEDGWLLVALDYSQIELRVLAHLSGDENLIRVFQEG
                     RDIHTETASWMFGVPREAVDPLMRRAAKTINFGVLYGMSAHRLSQELAIPYEEAQAFIE
                     RYFQSFPKVRAWIEKTLEEGRRRGYVETLFGRRRYVPDLEARVKSVREAAERMAFNMPV
                     QGTAADLMKLAMVKLFPRLEEMGARMLLQVHDELVLEAPKERAEAVARLAKEVMEGVYP
                     LAVPLEVEVGIGEDWLSAKE"
     CDS             2668..2685
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2738..2756)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2752..2799
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2836..3291
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2923..2942)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3133..3154
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3383..4198)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(3383..4198)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     4106..4125
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     4190..4209
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4320..4908
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4809..4828
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5062..5079
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5094..5233
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5159..5178)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5319..5341
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5335..5526)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(6335..7417)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7379..7398
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(7418..7495)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     7701..7720
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     join(7804..7822,1)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7804..7822
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact ttaagaagga
       61 gatataccat gggcagcagc catcatcatc atcatcacag cagcggcctg gtgccgcgcg
      121 gcagccatat gagggggatg ctgcccctct ttgagcccaa gggccgggtc ctcctggtgg
      181 acggccacca cctggcctac cgcaccttcc acgccctgaa gggcctcacc accagccggg
      241 gggagccggt gcaggcggtc tacggcttcg ccaagagcct cctcaaggcc ctcaaggagg
      301 acggggacgc ggtgatcgtg gtctttgacg ccaaggcccc ctccttccgc cacgaggcct
      361 acggggggta caaggcgggc cgggccccca cgccggagga ctttccccgg caactcgccc
      421 tcatcaagga gctggtggac ctcctggggc tggcgcgcct cgaggtcccg ggctacgagg
      481 cggacgacgt cctggccagc ctggccaaga aggcggaaaa ggagggctac gaggtccgca
      541 tcctcaccgc cgacaaagac ctttaccagc tcctttccga ccgcattcac gtcctccacc
      601 ccgaggggta cctcatcacc ccggcctggc tttgggaaaa gtacggcctg aggcccgacc
      661 agtgggccga ctaccgggcc ctgaccgggg acgagtccga caaccttccc ggggtcaagg
      721 gcatcgggga gaagacggcg aggaagcttc tggaggagtg ggggagcctg gaagccctcc
      781 tcaagaacct ggaccggctg aagcccgcca tccgggagaa gatcctggcc cacatggacg
      841 atctgaagct ctcctgggac ctggccaagg tgcgcaccga cctgcccctg gaggtggact
      901 tcgccaaaag gcgggagccc gaccgggaga ggcttagggc ctttctggag aggcttgagt
      961 ttggcagcct cctccacgag ttcggccttc tggaaagccc caaggccctg gaggaggccc
     1021 cctggccccc gccggaaggg gccttcgtgg gctttgtgct ttcccgcaag gagcccatgt
     1081 gggccgatct tctggccctg gccgccgcca gggggggccg ggtccaccgg gcccccgagc
     1141 cttataaagc cctcagggac ctgaaggagg cgcgggggct tctcgccaaa gacctgagcg
     1201 ttctggccct gagggaaggc cttggcctcc cgcccggcga cgaccccatg ctcctcgcct
     1261 acctcctgga cccttccaac accacccccg agggggtggc ccggcgctac ggcggggagt
     1321 ggacggagga ggcgggggag cgggccgccc tttccgagag gctcttcgcc aacctgtggg
     1381 ggaggcttga gggggaggag aggctccttt ggctttaccg ggaggtggag aggccccttt
     1441 ccgctgtcct ggcccacatg gaggccacgg gggtgcgcct ggacgtggcc tatctcaggg
     1501 ccttgtccct ggaggtggcc gaggagatcg cccgcctcga ggccgaggtc ttccgcctgg
     1561 ccggccaccc cttcaacctc aactcccggg accagctgga aagggtcctc tttgacgagc
     1621 tagggcttcc cgccatcggc aagacggaga agaccggcaa gcgctccacc agcgccgccg
     1681 tcctggaggc cctccgcgag gcccacccca tcgtggagaa gatcctgcag taccgggagc
     1741 tcaccaagct gaagagcacc tacattgacc ccttgccgga cctcatccac cccaggacgg
     1801 gccgcctcca cacccgcttc aaccagacgg ccacggccac gggcaggcta agtagctccg
     1861 atcccaacct ccagaacatc cccgtccgca ccccgcttgg gcagaggatc cgccgggcct
     1921 tcatcgccga ggatgggtgg ctattggtgg ccctggacta tagccagata gagctcaggg
     1981 tgctggccca cctctccggc gacgagaacc tgatccgggt cttccaggag gggcgggaca
     2041 tccacacgga gaccgccagc tggatgttcg gcgtcccccg ggaggccgtg gaccccctga
     2101 tgcgccgggc ggccaagacc atcaacttcg gggtcctcta cggcatgtcg gcccaccgcc
     2161 tctcccagga gctagccatc ccttacgagg aggcccaggc cttcattgag cgctactttc
     2221 agagcttccc caaggtgcgg gcctggattg agaagaccct ggaggagggc aggaggcggg
     2281 ggtacgtgga gaccctcttc ggccgccgcc gctacgtgcc agacctagag gcccgggtga
     2341 agagcgtgcg ggaggcggcc gagcgcatgg ccttcaacat gcccgtccag ggcaccgccg
     2401 ccgacctcat gaagctggct atggtgaagc tcttccccag gctggaggaa atgggggcca
     2461 ggatgctcct tcaggtccac gacgagctgg tcctcgaggc cccaaaagag agggcggagg
     2521 ccgtggcccg gctggccaag gaggtcatgg agggggtgta tcccctggcc gtgcccctgg
     2581 aggtggaggt ggggataggg gaggactggc tctccgccaa ggagtgagaa ttcgagctcc
     2641 gtcgacaagc ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct
     2701 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
     2761 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
     2821 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2881 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     2941 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3001 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     3061 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3121 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     3181 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3241 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     3301 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     3361 gtatccgctc atgaattaat tcttagaaaa actcatcgag catcaaatga aactgcaatt
     3421 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
     3481 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
     3541 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
     3601 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt
     3661 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
     3721 aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag
     3781 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
     3841 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg
     3901 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
     3961 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
     4021 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga
     4081 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
     4141 ccatgttgga atttaatcgc ggcctagagc aagacgtttc ccgttgaata tggctcataa
     4201 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgac caaaatccct
     4261 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4321 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4381 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4441 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     4501 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4561 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4621 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     4681 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     4741 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     4801 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     4861 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     4921 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     4981 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5041 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     5101 cggtattttc tccttacgca tctgtgcggt atttcacacc gcaatggtgc actctcagta
     5161 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     5221 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5281 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5341 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     5401 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     5461 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     5521 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     5581 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     5641 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     5701 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     5761 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     5821 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     5881 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     5941 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     6001 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     6061 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     6121 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     6181 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     6241 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     6301 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6361 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6421 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     6481 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     6541 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     6601 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     6661 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     6721 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     6781 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     6841 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     6901 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     6961 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     7021 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     7081 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     7141 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     7201 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     7261 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     7321 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     7381 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     7441 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     7501 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     7561 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     7621 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     7681 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     7741 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     7801 aattaatacg actcactata gg
//
