LOCUS       sequence-329825-        5856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast integrative plasmid for expressing wild-type Murine 
            polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic 
            marker (K. lactis URA3)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Cheah LC, Stark T, Adamson LSR, Abidin RS, Lau YH, Sainsbury F, 
            Vickers CE
  TITLE     Artificial Self-assembling Nanocompartment for Organizing Metabolic 
            Pathways in Yeast.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3251-3263. doi: 
            10.1021/acssynbio.1c00045. Epub 2021 Sep 30.
  PUBMED    34591448
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00045"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3251-3263"
COMMENT     Sequence Label: wtVP1 only
FEATURES             Location/Qualifiers
     source          1..5856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(236..252)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(236..252)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(249..271)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    260..276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(284..290,291..308,309..314))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    329..350
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(467..484)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(638..1226)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(718..737)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1397..2188,2189..2257))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2020..2039
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2258..2362)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2430..2448
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2486..2508)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2608..2627
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2821..2843
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2835..2852
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2836..2852
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3966..3999)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4232..4673
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    4232..4349
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     4644..4667
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 actagtagga ctatttgcaa agggaaggga tgctaaggta gagggtgaac gttacagaaa
       61 agcaggctgg gaagcatatt tgagaagatg cggccagcaa aactaaaaaa ctgtattata
      121 agtaaatgca tgtatactaa actcacaaat tagagcttca atttaattat atcagttatt
      181 acccgggaat ctcggtcgta atgatttaaa tgcatgcaag cttggcgtaa tcatggtcat
      241 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      301 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      361 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      421 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
      481 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      541 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      601 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      661 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      721 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      781 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      841 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      901 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      961 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1021 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1081 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1141 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1201 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1261 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     1321 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     1381 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     1441 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     1501 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     1561 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     1621 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     1681 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     1741 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     1801 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     1861 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     1921 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     1981 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     2041 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     2101 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     2161 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     2221 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     2281 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     2341 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     2401 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     2461 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     2521 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     2581 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     2641 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     2701 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     2761 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     2821 cccagtcacg acgttgtaaa acgacggcca gtgaattcat ttaaattttt ttgattcggt
     2881 aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg tatatatacg
     2941 catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac tgcacagaac
     3001 aaaaacctgc aggaaacgaa gataaaacat gtccacaaaa tcatatacca gtagagctga
     3061 gactcatgca agtccggttg catcgaaact tttacgttta atggatgaaa agaagaccaa
     3121 tttgtgtgct tctcttgacg ttcgttcgac tgatgagcta ttgaaacttg ttgaaacgtt
     3181 gggtccatac atttgccttt tgaaaacaca cgttgatatc ttggatgatt tcagttatga
     3241 gggtactgtc gttccattga aagcattggc agagaaatac aagttcttga tatttgagga
     3301 cagaaaattc gccgatatcg gtaacacagt caaattacaa tatacatcgg gcgtttaccg
     3361 tatcgcagaa tggtctgata tcaccaacgc ccacggggtt actggtgctg gtattgttgc
     3421 tggcttgaaa caaggtgcgc aagaggtcac caaagaacca aggggattat tgatgcttgc
     3481 tgaattgtct tccaagggtt ctctagcaca cggtgaatat actaagggta ccgttgatat
     3541 tgcaaagagt gataaagatt tcgttattgg gttcattgct cagaacgata tgggaggaag
     3601 agaagaaggg tttgattggc taatcatgac cccaggtgta ggtttagacg acaaaggcga
     3661 tgcattgggt cagcagtaca gaaccgtcga cgaagttgta agtggtggat cagatatcat
     3721 cattgttggc agaggacttt tcgccaaggg tagagatcct aaggttgaag gtgaaagata
     3781 cagaaatgct ggatgggaag cgtaccaaaa gagaatcagc gctccccatt aattatacag
     3841 gaaacttaat agaacaaatc acatatttaa tctaatagcc acctgcattg gcacggtgca
     3901 acactacttc aacttcatct tacaaaaaga tcacgtgatc tgttgtattg ggatctctag
     3961 acctaataac ttcgtatagc atacattata cgaagttata ttaagggttg ctcgacttat
     4021 attgaatttt caaaaattct tacttttttt ttggatggac gcaaagaagt ttaataatca
     4081 tattacatgg caataccacc atatacatat ccatatctaa tcttacttat atgttgtgga
     4141 aatgtaaaga gccccattat cttagcctaa aaaaaccttc tctttggaac tttcagtaat
     4201 acgcttaact gctcattgct atattgaagt acggattaga agccgccgag cgggcgacag
     4261 ccctccgacg gaagactctc ctccgtgcgt cctggtcttc accggtcgcg ttcctgaaac
     4321 gcagatgtgc ctcgcgccgc actgctccga acaataaaga ttctacaata ctagctttta
     4381 tggttatgaa gaggaaaaat tggcagtaac ctggccccac aaaccttcaa atcaacgaat
     4441 caaattaaca accataggat aataatgcga ttagtttttt agccttattt ctggggtaat
     4501 taatcagcga agcgatgatt tttgatctat taacagatat ataaatgcaa aagctgcata
     4561 accactttaa ctaatacttt caacattttc ggtttgtatt acttcttatt caaatgtcat
     4621 aaaagtatca acaaaaaatt gttaatatac ctctatactt taacgtcaag gagaaaaaac
     4681 tatagctagc aaaatggctc caaagagaaa gtcaggtgta agtaaatgcg aaaccaaatg
     4741 cacaaaggct tgccctagac cagctccagt tcctaagtta ttgattaaag gtggtatgga
     4801 agttttggat ttggttacag gtccagattc tgttactgaa atcgaagcat ttttgaaccc
     4861 aagaatgggt caaccaccaa caccagaatc tttaactgaa ggtggtcaat attacggttg
     4921 gtcaagaggt attaatttgg ctacatctga tactgaagat tcaccaggta ataatacatt
     4981 accaacttgg tctatggcaa aattgcaatt accaatgttg aacgaagatt tgacttgtga
     5041 tactttgcaa atgtgggaag cagtttcagt taaaacagaa gttgttggtt ctggttcttt
     5101 gttggatgtt catggtttta ataagccaac agatactgtt aacacaaagg gtatttctac
     5161 tccagttgaa ggttcacaat atcatgtttt tgctgttggt ggtgaaccat tggatttgca
     5221 aggtttagtt acagatgcaa gaactaagta caaggaagaa ggtgttgtta ctattaaaac
     5281 aatcactaag aaagatatgg ttaataagga tcaagttttg aacccaattt caaaggctaa
     5341 attggataag gatggcatgt acccagttga aatttggcat ccagatccag ctaaaaatga
     5401 aaacacaaga tacttcggta attacactgg tggtactaca actccaccag ttttgcaatt
     5461 cactaacact ttgacaactg ttttgttaga tgaaaatggt gttggtccat tatgtaaagg
     5521 tgaaggtttg tacttatctt gcgttgatat tatgggttgg agagttacaa gaaactacga
     5581 tgttcatcat tggagaggtt tgccaagata cttcaagatt actttaagaa aaagatgggt
     5641 taaaaatcca tacccaatgg cttctttgat ttcttctttg tttaataata tgttaccaca
     5701 agttcaaggt caaccaatgg aaggtgaaaa cacacaagtt gaagaagtta gagtttacga
     5761 tggtactgaa ccagttccag gtgaccctga catgacaaga tatgtagata gattcggtaa
     5821 aactaaaact gtattccctg gtaattaagc ggccgc
//
