LOCUS       sequence-329841-        3501 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri SLC25A22.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-SLC25A22
FEATURES             Location/Qualifiers
     source          1..3501
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1626..1725)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1742..1761)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1743..1761)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1766..1782)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1766..1782)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1884..1903)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1895..2704
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1962..1981)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2851..3439
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3340..3359
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgat ggctgataaa cagatcagtt tgcctgccaa
      721 attgattaat ggagggatcg ccggcctgat tggagtgacc tgtgtgtttc ccattgacct
      781 ggccaagact cgtctgcaaa accagcaaaa tggatctcgc ctttatacca gcatgtcaga
      841 ttgccttatt aagaccatca ggtcagaggg gtacttcggg atgtatcgag gagctgcggt
      901 gaacctaacg ctagtcacac cagagaaagc cattaaattg gctgctaacg acttcttcag
      961 gcatcacctc tctaaggatg gaaaactcac tctgctcaaa gagatgctgg ccgggtgtgg
     1021 ggcaggtaca tgccaggtta ttgtaacaac tcctatggag atgctgaaaa tccagcttca
     1081 ggatgctgga aggcttgctg ctcagaggaa gctcatgccg gagacggtgg ctgcaggcac
     1141 cgtggagacc aagtccccca cagccatgca gctaaccaga caattactga gggaaaaggg
     1201 catagctggg ctttataagg gcctcggtgc cacattgctc agggacgttc ctttctccat
     1261 catctatttc cccctttttg ccaacctgaa taacttgggc aaacggggcg ccgagggccc
     1321 agctcctttc tacgtgtcgt ttgtctccgg ctgcgttgca ggaagcacag cggccgttgc
     1381 tgtcaacccc gttgacgtaa taaagactcg gctgcagtcc ctgaaccgag cgagcacaga
     1441 agacacgtac agtggagtga ccgactgcat caggaaaatc atgcgcaacg agggtccggc
     1501 ggccttcctg aagggagcgt actgtcgagc cctggtcatt gcaccgctgt tcggcattgc
     1561 acaggtggta tacttcctgg gcgtggggga atttatcctc agcttcttgc ctaaaaaaga
     1621 caacgaccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
     1681 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat
     1741 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc
     1801 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact
     1861 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc
     1921 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga
     1981 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga
     2041 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag
     2101 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc
     2161 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga
     2221 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt
     2281 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca
     2341 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa
     2401 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga
     2461 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt
     2521 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat
     2581 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata
     2641 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt
     2701 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga
     2761 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     2821 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2881 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2941 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3001 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3061 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3121 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3181 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3241 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3301 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3361 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3421 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3481 ctggcctttt gctcacatgt t
//
