LOCUS       sequence-329842-        3960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri SMPDL3B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-SMPDL3B
FEATURES             Location/Qualifiers
     source          1..3960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2085..2184)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2201..2220)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2202..2220)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2225..2241)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2225..2241)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2343..2362)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2354..3163
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2421..2440)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3310..3898
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3799..3818
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgat gtattttgct gtcccagagt ccaacatgtc
      721 tgcagtatgg atcctcattt cttctctgct tttcaaggag ggtcttgcgc tgtcagggag
      781 cttttggcat atcaccgatt tgcattggga tccaacgttt aaactaacgg agaacccaga
      841 gtcagtgtgt gcgtccagcg gaaaacgacc agcgcctggt gctggtatat ttggagacta
      901 tgtgtgtgac tcaccctggc acctgatcaa ctcttctgtt tacgccatga agcatattct
      961 accagagccg gacttcatcg tatggacagg tgacgacaca ccgcatgtac caaatgagga
     1021 tctaggagag gaggcagtgg tcagcattat cagcaacctt actcatatca taaaggaggt
     1081 ttttccaact actaaagtgt actctgctct gggtaaccat gactaccacc ccaagagtca
     1141 gctacctcca tcagcaaact ccatctatga cagagtaaca gaaatgtggc gagattggtt
     1201 gaatccacag tctgaagaaa tgtttagaaa aggtgggttt tacaccgaaa agctgcttaa
     1261 ccgaacaggc tttaggattc tggtcctcaa cactaatctc tactacgacc agaacaaggt
     1321 gacccagagc acggatgacc cagcaggcca gtttagctgg atggattgga ctcttacaga
     1381 ggcagccaac aaaagcgaga aggtgtacct cattggccat gttcctcctg gtttctttga
     1441 gaagaaacga aacaagccgt ggttcacctc aaagttcaac aaacaatatc tggacttaat
     1501 agagaagcat cattgtgtca tagcagggca gttcttcggc catcaccata ctgacagctt
     1561 ccgcatgttc tacagcgcag aaggtgtgcc catcagcaca atgtttctca gcccaggggt
     1621 aaccccatgg gaaacgacac ttcctggagt ctcgaatgga gccaacaacc ctgggattcg
     1681 tgtgtttgaa tatgacacgg aaacactcct agtaaaagat gtggtgacat attatctaaa
     1741 cctcacgtac gctaacaccg tcacggagcg ctgggaaaaa gagtatcgcc tcacagagag
     1801 tttccgagta gcagacgcct ctccggcctc catgcacctg gtcctggagc gaatggctgc
     1861 agatccctgc tacctgcaga agtactatga tttcaactca gtcagctatg acctgacgaa
     1921 ctgtgacggc gactgccgtg ttgatcacgt atgtgcagca agagaagtag attttgacag
     1981 gtatgaggag tgtctggtaa aagagggagt ggattctatt aaaggtgggc tgctgctgct
     2041 ggtcctttct gtgggtgtga gcatcacatc agcagccttg cacgacccag ctttcttgta
     2101 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     2161 cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta
     2221 catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat
     2281 tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa
     2341 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
     2401 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
     2461 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
     2521 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
     2581 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
     2641 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
     2701 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
     2761 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
     2821 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
     2881 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
     2941 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
     3001 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     3061 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     3121 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     3181 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     3241 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3301 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3361 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3421 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     3481 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3541 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3601 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3661 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3721 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3781 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3841 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3901 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
//
