LOCUS       sequence-329850-        1698 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector carrying the MCP-ADAR2 with a nuclear export 
            signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Katrekar D, Xiang Y, Palmer N, Saha A, Meluzzi D, Mali P
  TITLE     Comprehensive interrogation of the ADAR2 deaminase domain for 
            engineering enhanced RNA editing activity and specificity.
  JOURNAL   Elife. 2022 Jan 19;11. pii: 75555. doi: 10.7554/eLife.75555.
  PUBMED    35044296
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-01-19"; volume: "11. pii: 75555. doi"; pages: " 
            10.7554/eLife.75555"
COMMENT     Sequence Label: MCP-ADAR2-DD
FEATURES             Location/Qualifiers
     source          1..1698
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1606..1632
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             1642..1698
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gccaccatgg cgtccaattt cactcagttt gtgctggttg acaacggcgg gaccggggac
       61 gttacggtag ccccctcaaa ctttgccaac ggtatagcgg agtggataag cagcaattct
      121 aggagtcaag catacaaagt tacatgcagc gtgcgccaat ctagcgctca gaatcgcaag
      181 tacaccatta aagtagaggt ccccaaggga gcctggagaa gctatcttaa catggagttg
      241 accataccaa tcttcgctac caactctgac tgtgaactca ttgtgaaagc catgcaaggt
      301 ctgctcaagg atggtaaccc aattccgtcc gctatcgctg ccaactctgg gatttacggg
      361 ggcagtggga gcggtgcagg atctggtagt ccagctgggg gaggagcacc gggtagcggt
      421 ggggggtctc agctccatct gccgcaagtt ctggctgatg ccgtgagcag actcgtgcta
      481 ggcaaattcg gcgacctgac cgacaacttc agcagccctc acgccagaag aaaagtcctg
      541 gccggcgtgg tcatgacaac cggcacagat gtgaaggacg ccaaagtgat cagcgtgtcc
      601 accggcacca agtgcatcaa cggcgagtac atgagcgaca gaggactggc cctgaacgat
      661 tgccacgccg agatcatcag cagaagaagc ctgctgcggt tcctatacac ccagctggaa
      721 ctgtacctga acaacaagga cgaccagaag cggtccatct tccagaagtc tgagagaggc
      781 ggcttccggc tgaaagaaaa tgtgcagttt cacctataca tctccacaag cccttgcggc
      841 gacgctcgga tctttagccc ccacgagccc atcctggaag aacctgccga cagacacccc
      901 aacagaaagg cccggggcca gctgagaaca aagatcgaaa gcggcgaagg cacaatcccc
      961 gtgcggagca atgcctctat ccaaacatgg gacggcgtgc tgcaaggcga gagactgctg
     1021 acaatgagct gcagcgacaa gatcgcccgg tggaatgttg tgggcatcca gggatctctg
     1081 ctgagcatct tcgtggaacc catctacttc tcgagcatca tcctgggcag cctgtaccac
     1141 ggcgatcacc tgagcagagc catgtaccag cggatcagca atatcgagga cctgcctcct
     1201 ctgtataccc tgaacaagcc actgctgagc ggcatctcca atgccgaagc tagacagcct
     1261 ggcaaggccc ctaacttcag cgtgaactgg acagtggggg atagcgccat cgaagtgatc
     1321 aatgccacca ccggcaaaga cgaactagga agagccagca gactgtgcaa gcacgccctg
     1381 tattgcagat ggatgagagt gcatgggaaa gtgccttctc atctgctgag aagcaagatc
     1441 accaagccta acgtgtacca cgagagcaag ctggccgcta aagagtacca ggccgccaag
     1501 gccagactgt tcaccgcctt tattaaggcc ggactcggcg cctgggtcga gaagcctaca
     1561 gagcaggatc agttcagcct gacacctggg agcggatccg gctctctgcc accattggaa
     1621 aggcttacgc tgggatccgg cgcaacaaac ttctctctgc tgaaacaagc cggagatgtc
     1681 gaagagaatc ctggaccg
//
