LOCUS       sequence-329852-        4326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry vector for N. furzeri TM9SF3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Chen YR, Harel I, Singh PP, Ziv I, Moses E, Goshtchevsky U, Machado 
            BE, Brunet A, Jarosz DF
  TITLE     Tissue-specific landscape of protein aggregation and quality control
            in an aging vertebrate.
  JOURNAL   Dev Cell. 2024 Jul 22;59(14):1892-1911.e13. doi: 
            10.1016/j.devcel.2024.04.014. Epub 2024 May 28.
  PUBMED    38810654
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2024.04.014"; journalName: "Dev Cell"; date: 
            "2024-07-22- 22"; volume: "59"; issue: "14"; pages: "1892-1911.e13"
COMMENT     Sequence Label: pDONR221-TM9SF3
FEATURES             Location/Qualifiers
     source          1..4326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      1863..1872
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(2451..2550)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2567..2586)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2568..2586)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2591..2607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2591..2607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2709..2728)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2720..3529
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2787..2806)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3676..4264
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4165..4184
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gaaggagata acaaaatgat gatgggatct tcttggtgga aggtggcagc
      721 ggcggcgttt ttagctgtag ctggattttt attgcctgtt gacgcagacg agcatgacca
      781 cacgtacaca gataaggagg aagtagttct atggatgaac acggtggggc cttaccacaa
      841 ccgacaggag acatacaagt atttctcttt gcccttctgt gcgggctcta agaagaccat
      901 cagtcattac catgaaaccc tgggagaggc tctgcagggc gtggagctgg aattcagtgg
      961 gttggacatc aagtttaaag aggaagtcat gcagaacacg tattgtgaaa ttgaaatgga
     1021 caaagcaaag caggatgcct ttgtttatgc cataaagaac cattattggt accaaatgta
     1081 catagatgac ctgcccatct ggggtattgt tggtgaggca gatgaaaacg gagaagagca
     1141 ttacctgtgg acgtataaga aactggagat cggctacaac ggcaacagaa ttgttgacgt
     1201 aaacctgacg agtgaaggca aagtcaaact catgcccaac actaggattg ctatgtctta
     1261 ctctgtcaaa tggaagaagt cggatgtgaa gtttgaggac agattcgaca agtacctcga
     1321 tccgtctttt ttccagcaca ggattcattg gttctcaatt tttaactcct tcatgatggt
     1381 gattttcttg gtgggccttg tgtccatgat cctcatgaga accttaagaa aggattatgc
     1441 caggtacagt aaagaagagg aaatggacga catggaccga gacctgggcg acgaatacgg
     1501 gtggaagcag gtgcatggag acgtgttcag gccttcaagc catccactga tcttctcctc
     1561 gctcattggc tctggctgcc agatcttctc tgtctccttc atcgtcatca tcgtggcgat
     1621 ggttgaagat ctgtacacag agaggggctc catgctgagc acagcgatct tcgtgtacgc
     1681 tgcaacctcc cctgtcaacg gctactttgg gggaagttta tatgcaaaac aaggaggaag
     1741 aagatggatc aaacaaatgt tcattggggc gtttatgatc cctgctatgg tgtgtgggac
     1801 ggccttcttc atcaacttca ttgccatcta ctaccacgcc tctagagcca tcccatttgg
     1861 caccatggtg gctgtctgct gtatctgttt ctttgtcatc ctgcctctaa atcttgtggg
     1921 aacgattttg ggaaggaacc tctccggcca gccaaacttc ccctgccgag tcaacgccgt
     1981 gccgcggcca atccctgaga agaaatggtt catggaacca gctgtcatcg tgtgcctcgg
     2041 aggaatcttg ccatttggtt ccatttttat agaaatgtac ttcatcttca cgtccttttg
     2101 ggcctataag atctactacg tgtacggctt tatgatgctg gtcctggtca tcttgtgtat
     2161 cgtcaccgtg tgcgtgacca tcgtgtgtac gtacttcctg ctcaacgctg aggactacag
     2221 atggcagtgg acaagcttcc tctcagctgc gtccacggca gtttatgttt acatgtactc
     2281 cttttactac tacttcttca agactaagat gtacgggttg tttcagacgt ccttctactt
     2341 cggctacatg gccgtgttca gcactgctct gggaatcatg tgtggtgccg tgggctacat
     2401 gggaacaagt gcctttgtga agaagatcta cacaaatgtg aaaatcgacg acccagcttt
     2461 cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca acgaacaggt
     2521 cactatcagt caaaataaaa tcattatttg ccatccagct gatatcccct atagtgagtc
     2581 gtattacatg gtcatagctg tttcctggca gctctggccc gtgtctcaaa atctctgatg
     2641 ttacattgca caagataaaa atatatcatc atgaacaata aaactgtctg cttacataaa
     2701 cagtaataca aggggtgtta tgagccatat tcaacgggaa acgtcgaggc cgcgattaaa
     2761 ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc
     2821 aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca
     2881 tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac
     2941 ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt
     3001 actcaccact gcgatccccg gaaaaacagc attccaggta ttagaagaat atcctgattc
     3061 aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt
     3121 ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat
     3181 gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga
     3241 acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca
     3301 tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga
     3361 tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct
     3421 cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc
     3481 tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat cagaattggt
     3541 taattggttg taacactggc agagcattac gctgacttga cgggacggcg caagctcatg
     3601 accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa
     3661 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3721 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3781 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3841 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3901 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3961 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     4021 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4081 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4141 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4201 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4261 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4321 catgtt
//
