LOCUS       sequence-329863-        5765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast integrative plasmid for expressing P. syringae uronate 
            dehydrogenase, with TEF1 promoter. Contains leucine auxotrophic 
            marker (K. lactis LEU2)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Cheah LC, Stark T, Adamson LSR, Abidin RS, Lau YH, Sainsbury F, 
            Vickers CE
  TITLE     Artificial Self-assembling Nanocompartment for Organizing Metabolic 
            Pathways in Yeast.
  JOURNAL   ACS Synth Biol. 2021 Dec 17;10(12):3251-3263. doi: 
            10.1021/acssynbio.1c00045. Epub 2021 Sep 30.
  PUBMED    34591448
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00045"; journalName: "ACS Synth Biol"; date: 
            "2021-12-17- 17"; volume: "10"; issue: "12"; pages: "3251-3263"
COMMENT     Sequence Label: pTEF1-UDH
FEATURES             Location/Qualifiers
     source          1..5765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1876..2289
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      3157..3404
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3173..3191)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        complement(3418..3825)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(3839..3856)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4010..4598)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4090..4109)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4769..5560,5561..5629))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5392..5411
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5630..5734)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttaaatca atattaatgt taaagtgcaa ttctttttcc ttatcacgtt gagccattag
       61 tatcaatttg cttacctgta ttcctttaca tcctcctttt tctccttctt gataaatgta
      121 tgtagattgc gtatatagtt tcgtctaccc tatgaacata ttccattttg taatttcgtg
      181 tcgtttctat tatgaatttc atttataaag tttatgtaca aatatcataa aaaaagagaa
      241 tctttttaag ccaagatttc cttgacagcc ttggcgatag catcgccaac ctcagtggta
      301 gagttagaac caccaaggtc accggttctg acacctgcat ccaagacatt tctaacagct
      361 tcttcaagag ccctaccttc ttcaaccaaa tccaaggata acttcaacat catagctgca
      421 gataagatgg tagcaattgg gttaaccttg tttgctggta aatctggggc agaaccatga
      481 catggttcgt acaaaccgaa tgccttgtta gtgtcaggta gggaagctag agatgcagaa
      541 ggtaataaac ccaaagaacc tggaataaca gaggcttcat cggagataat atcaccaaac
      601 atgttgttgg taataacaac accgtttagc ttagttggtg atttaaccaa aatcatagca
      661 gcagagtcga tcaattggtg ctgaacagtt aattgtggga actcagtctt gatggtttct
      721 tcaacagtct ttctccacaa tctggaagag gcaagcacgt tagccttgtc aagagaccag
      781 attggtaatg gtgggttttg ttgcaatgcc aagaaagcag ccattcttgt aattctttga
      841 acttcaggaa cactgtattt ctcagagtcc caagcaactc cgtcaccttc atcttctttt
      901 ctttcaccaa agtagatacc accaaccaat tctctaacga cgacgaaatc ggtacccttt
      961 gcatattcag gcttcaaagg agaaagatct agtaaagaat cagaagcaaa gttacatggt
     1021 ctcaagttgg cgtatagacc caattccttt ctgatcttca atagaccttg ttctggtcta
     1081 actgcgcccg taccccattt tggaccacca acagcaccta gtaagacagc atcggctttc
     1141 ttagaggctt ctagagcttc atctggtaaa ggagtgccag tggcatcgat ggcagcaccc
     1201 ccgatcaagt gatgttggaa attgaactta atttctggac ggacttcagc aatggcattc
     1261 aagaccttaa tagcttcgtc agtaacttct ttaccgacgt gatcacccgg taggacaacg
     1321 atattcttag acatggtata atctgtgtag tgtgggatac tttttacttc ttcaaatagg
     1381 tatcaacaat aaaaataaat cgaagcaaat gtaggaatgc gttaaagcag atgtacttac
     1441 ttagagtaca tagatatatt tatataattc aatatataaa agtatatgaa gcatctgatg
     1501 ttgaacctgt catgactcta aactggaatg ggtagttatg cttctgaagg ttttccgtta
     1561 gaaaatgagt gcagggatca ggcccggaac cggttttagc ctgaaaggaa aagaaaaaaa
     1621 tgcggtgatt actagcacgt gactgcgctg aattggaatc taccacaagt acagcgaatg
     1681 gcaagaggga aggggaatat attgtgcctg gcggtagcgt ctgctatatc aaatgttaaa
     1741 cataactaaa tgcagtagtg aatagagtaa tgtatttcta catatgtggg tagggccaat
     1801 gggagcccga tgtctattgt acagatattt tccttttata gttaatcaat tcaacttgga
     1861 aatcattgag tcgatgaatt cccacacacc atagcttcaa aatgtttcta ctcctttttt
     1921 actcttccag attttctcgg actccgcgca tcgccgtacc acttcaaaac acccaagcac
     1981 agcatactaa atttcccctc tttcttcctc tagggtgtcg ttaattaccc gtactaaagg
     2041 tttggaaaag aaaaaagaga ccgcctcgtt tctttttctt cgtcgaaaaa ggcaataaaa
     2101 atttttatca cgtttctttt tcttgaaaat tttttttttt gatttttttc tctttcgatg
     2161 acctcccatt gatatttaag ttaataaacg gtcttcaatt tctcaagttt cagtttcatt
     2221 tttcttgttc tattacaact ttttttactt cttgctcatt agaaagaaag catagcaatc
     2281 taatctaagt tttaattaca aactcgagaa aatggcatct gctcatacta ctcaaactcc
     2341 attcaatcgt ctactgctga ccggtgctgc aggcggcctg ggcaaggtat tgcgtgagac
     2401 attgcgtcct tactcacaca ttctgcgtct atctgatatc gccgagatgg ctcccgctgt
     2461 cggcgaccat gaagaagttc aggtctgcga tctggcggac aaagacgccg tacaccgtct
     2521 ggtcgaaggc gttgacgcca ttttgcattt tggcggcgtg tctgtcgaaa gacctttcga
     2581 ggaaatcttg ggcgctaata tctgcggcgt tttccatatc tacgaggccg cccgtcgtca
     2641 tggcgtgaaa cgtgtgatct tcgccagctc caaccatgtt atcggtttct acaagcagaa
     2701 cgaaaccatc gacgcgcact ccccacgtag gccagattct tactatggtt tgtccaagtc
     2761 ctacggcgaa gacatggcca gcttctactt tgatcgttac ggcatcgaaa ccgtcagcat
     2821 ccgtatcggc tcatccttcc ctgaaccaca gaaccgtaga atgatgagca cctggctgag
     2881 cttcgatgac ctgacccgtt tgctagagcg tgccctgtac acgccggacg tcggccacac
     2941 cgtggtgtat ggcgtgtctg acaacaagac cgtttggtgg gacaacagat ttgccagcaa
     3001 actggactac gcccctaaag acagctcgga ggtcttccgt gccaaggtcg acgcccagcc
     3061 aatgccagct gacgacgacc ctgcaatggt ttaccagggc ggtgcatttg tagcgtccgg
     3121 accgttcggc gataaataac ctgcaggagg gccgcatcat gtaattagtt atgtcacgct
     3181 tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg
     3241 aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat
     3301 ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa
     3361 ccttgcttga gaaggttttg ggacgctcga aggctttaat ttgcggcctg cggccgctag
     3421 aatggtatat ccttgaaata tatatatata tattgctgaa atgtaaaagg taagaaaagt
     3481 tagaaagtaa gacgattgct aaccacctat tggaaaaaac aataggtcct taaataatat
     3541 tgtcaacttc aagtattgtg atgcaagcat ttagtcatga acgcttctct attctatatg
     3601 aaaagccggt tccggcgctc tcacctttcc tttttctccc aatttttcag ttgaaaaagg
     3661 tatatgcgtc aggcgacctc tgaaattaac aaaaaatttc cagtcatcga atttgattct
     3721 gtgcgatagc gcccctgtgt gttctcgtta tgttgaggaa aaaaataatg gttgctaaga
     3781 gattcgaact cttgcatctt acgatacctg agtattccca cagttattta aatgcttcct
     3841 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3901 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3961 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4021 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4081 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4141 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4201 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4261 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4321 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4381 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4441 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4501 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4561 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4621 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4681 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4741 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4801 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4861 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4921 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4981 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     5041 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     5101 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5161 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5221 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5281 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5341 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5401 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5461 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5521 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5581 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5641 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5701 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     5761 acgtc
//
